scholarly journals Developmental toxicity from exposure to various forms of mercury compounds in medaka fish (Oryzias latipes) embryos

2016 ◽  
Author(s):  
Wu Dong ◽  
Jie Liu ◽  
Lixin Wei ◽  
Yang Jingfeng ◽  
Melissa Chernick ◽  
...  
Keyword(s):  
Developmental Toxicity ◽  
Inorganic Mercury ◽  
Oryzias Latipes ◽  
Heme Oxygenase 1 ◽  
Organic Form ◽  
Traditional Medicines ◽  
Mercury Compounds ◽  
Pericardial Edema ◽  
Eye Pigmentation ◽  
Swim Bladder Inflation

This study examined developmental toxicity of different mercury compounds, including some used in traditional medicines. Medaka (Oryzias latipes) embryos were exposed to 0.001-10 μM concentrations of MeHg, HgCl2, α-HgS (Zhu Sha), and β-HgS (Zuotai) from stage 10 (6-7 hpf) to 10 days post fertilization (dpf). Of the forms of mercury in this study, the organic form (MeHg) proved the most toxic followed by inorganic mercury (HgCl2), both producing embryo developmental toxicity. Altered phenotypes included pericardial edema with elongated or tube heart, reduction of eye pigmentation, and failure of swim bladder inflation. Both α-HgS and β-HgS were less toxic than MeHg and HgCl2. Total RNA was extracted from survivors 3 days after exposure to MeHg (0.1 μM), HgCl2 (1 μM), α-HgS (10 μM), or β-HgS (10 μM) to examine toxicity-related gene expression. MeHg and HgCl2 markedly induced metallothionein (MT) and heme oxygenase-1 (Ho-1), while α-HgS and β-HgS failed to induce either gene. Chemical forms of mercury compounds proved to be a major determinant in their developmental toxicity.

scholarly journals Developmental toxicity from exposure to various forms of mercury compounds in medaka fish (Oryzias latipes) embryos

2016 ◽  
Author(s):  
Wu Dong ◽  
Jie Liu ◽  
Lixin Wei ◽  
Yang Jingfeng ◽  
Melissa Chernick ◽  
...  
Keyword(s):  
Developmental Toxicity ◽  
Inorganic Mercury ◽  
Oryzias Latipes ◽  
Heme Oxygenase 1 ◽  
Organic Form ◽  
Traditional Medicines ◽  
Mercury Compounds ◽  
Pericardial Edema ◽  
Eye Pigmentation ◽  
Swim Bladder Inflation

This study examined developmental toxicity of different mercury compounds, including some used in traditional medicines. Medaka (Oryzias latipes) embryos were exposed to 0.001-10 μM concentrations of MeHg, HgCl2, α-HgS (Zhu Sha), and β-HgS (Zuotai) from stage 10 (6-7 hpf) to 10 days post fertilization (dpf). Of the forms of mercury in this study, the organic form (MeHg) proved the most toxic followed by inorganic mercury (HgCl2), both producing embryo developmental toxicity. Altered phenotypes included pericardial edema with elongated or tube heart, reduction of eye pigmentation, and failure of swim bladder inflation. Both α-HgS and β-HgS were less toxic than MeHg and HgCl2. Total RNA was extracted from survivors 3 days after exposure to MeHg (0.1 μM), HgCl2 (1 μM), α-HgS (10 μM), or β-HgS (10 μM) to examine toxicity-related gene expression. MeHg and HgCl2 markedly induced metallothionein (MT) and heme oxygenase-1 (Ho-1), while α-HgS and β-HgS failed to induce either gene. Chemical forms of mercury compounds proved to be a major determinant in their developmental toxicity.

scholarly journals Developmental toxicity from exposure to various forms of mercury compounds in medaka fish (Oryzias latipes) embryos

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2282 ◽  
Author(s):  
Wu Dong ◽  
Jie Liu ◽  
Lixin Wei ◽  
Yang Jingfeng ◽  
Melissa Chernick ◽  
...  
Keyword(s):  
Developmental Toxicity ◽  
Inorganic Mercury ◽  
Oryzias Latipes ◽  
Heme Oxygenase 1 ◽  
Organic Form ◽  
Traditional Medicines ◽  
Mercury Compounds ◽  
Pericardial Edema ◽  
Eye Pigmentation ◽  
Swim Bladder Inflation

This study examined developmental toxicity of different mercury compounds, including some used in traditional medicines. Medaka (Oryzias latipes) embryos were exposed to 0.001–10 µM concentrations of MeHg, HgCl2,α-HgS (Zhu Sha), andβ-HgS (Zuotai) from stage 10 (6–7 hpf) to 10 days post fertilization (dpf). Of the forms of mercury in this study, the organic form (MeHg) proved the most toxic followed by inorganic mercury (HgCl2), both producing embryo developmental toxicity. Altered phenotypes included pericardial edema with elongated or tube heart, reduction of eye pigmentation, and failure of swim bladder inflation. Bothα-HgS andβ-HgS were less toxic than MeHg and HgCl2. Total RNA was extracted from survivors three days after exposure to MeHg (0.1 µM), HgCl2(1 µM),α-HgS (10 µM), orβ-HgS (10 µM) to examine toxicity-related gene expression. MeHg and HgCl2markedly induced metallothionein (MT) and heme oxygenase-1 (Ho-1), whileα-HgS andβ-HgS failed to induce either gene. Chemical forms of mercury compounds proved to be a major determinant in their developmental toxicity.

Zebrafish embryos exposed to deltamethrin exhibit abnormalities despite induced expression of related genes (you, you-too, momo and u-boot)

2018 ◽  
Vol 35 (1) ◽  
pp. 11-19
Author(s):  
Kuder Reshma Shabnam ◽  
Dharmapuri Gangappa ◽  
Gundala Harold Philip
Keyword(s):  
Vital Role ◽  
Primary Objective ◽  
Chain Reaction ◽  
Environmental Persistence ◽  
Expression Of Genes ◽  
Pericardial Edema ◽  
Eye Pigmentation ◽  
Polymerase Chain ◽  
Relationship Of ◽  
The Relationship

Evaluation of the toxic effects of a widely used synthetic pyrethroid, deltamethrin (DM), was carried out in this study. This pesticide is preferred for pest control because of its low environmental persistence and toxicity. We investigated the expression pattern of four genes, namely, you ( you), yot ( you-too), momo ( mom) and ubo ( u-boot) during early development of zebrafish, that is, from 12 hpf to 48 hpf stages. These stages are selected as most of the important developmental aspects take place during this period. All four genes are known to play a vital role in development of notochord and somites. To understand the effect of DM on development, embryos of 4 hpf stage were exposed to two concentrations (100 and 200 µg/L) of DM, and observations were made at 12, 24 and 48 hpf stages. Our earlier studies have shown phenotypic abnormalities such as notochord bending, tail deformation, yolk sac and pericardial edema, lightening of body and eye pigmentation and interfered in somite patterning, during these stages of development. Understanding the relationship of phenotypic abnormalities with these four genes has been our primary objective. These four genes were analyzed by Reverse transcription (RT)-polymerase chain reaction and intensity of the bands has shown induction in their expression after exposure to the toxicant. In spite of the expression of genes, it was noticed that DM caused abnormalities. It can be said from the results that translational pathway could have been affected.

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