scholarly journals Detectability of circulating microRNAs in microRNA extracts with low purity and yield using quantitative real-time polymerase chain reaction: Supporting evidence

2020 ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Supporting Evidence ◽  
Chain Reaction ◽  
Circulating Micrornas ◽  
Polymerase Chain

scholarly journals Standardisierte mikroRNA-Analytik — die große Chance für Biomarkerstudien?

BIOspektrum ◽  
2020 ◽  
Vol 26 (6) ◽  
pp. 621-623
Author(s):  
Maria Fauth ◽  
Meike J. Saul
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Clinical Application ◽  
Reverse Transcription ◽  
Prognostic Biomarkers ◽  
Chain Reaction ◽  
Acceptance Criteria ◽  
Circulating Micrornas ◽  
Polymerase Chain

Abstract Circulating microRNAs (miRs) represent promising diagnostic and prognostic biomarkers for various diseases. Despite the high number of biomarker studies, the results of these studies are hardly reproducible. This makes it difficult to transfer the results into clinical application. In this context, acceptance criteria for the quantification of miRs by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) may help to standardize miR analysis and improve reproducibility.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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