scholarly journals Decision letter: The pioneer factor OCT4 requires the chromatin remodeller BRG1 to support gene regulatory element function in mouse embryonic stem cells

2016 ◽  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Regulatory Element ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Pioneer Factor ◽  
Gene Regulatory ◽  
Gene Regulatory Element ◽  
Element Function

scholarly journals The pioneer factor OCT4 requires the chromatin remodeller BRG1 to support gene regulatory element function in mouse embryonic stem cells

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Hamish W King ◽  
Robert J Klose
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Embryonic Stem Cells ◽  
Regulatory Element ◽  
Embryonic Stem ◽  
Cellular Reprogramming ◽  
Factor Binding ◽  
Pioneer Factor ◽  
Pioneer Factors ◽  
Element Function

Pioneer transcription factors recognise and bind their target sequences in inaccessible chromatin to establish new transcriptional networks throughout development and cellular reprogramming. During this process, pioneer factors establish an accessible chromatin state to facilitate additional transcription factor binding, yet it remains unclear how different pioneer factors achieve this. Here, we discover that the pluripotency-associated pioneer factor OCT4 binds chromatin to shape accessibility, transcription factor co-binding, and regulatory element function in mouse embryonic stem cells. Chromatin accessibility at OCT4-bound sites requires the chromatin remodeller BRG1, which is recruited to these sites by OCT4 to support additional transcription factor binding and expression of the pluripotency-associated transcriptome. Furthermore, the requirement for BRG1 in shaping OCT4 binding reflects how these target sites are used during cellular reprogramming and early mouse development. Together this reveals a distinct requirement for a chromatin remodeller in promoting the activity of the pioneer factor OCT4 and regulating the pluripotency network.

scholarly journals CpG island-mediated global gene regulatory modes in mouse embryonic stem cells

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Samuel Beck ◽  
Bum-Kyu Lee ◽  
Catherine Rhee ◽  
Jawon Song ◽  
Andrew J. Woo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cpg Island ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Regulatory

scholarly journals A gene regulatory network in mouse embryonic stem cells

2007 ◽  
Vol 104 (42) ◽  
pp. 16438-16443 ◽  
Author(s):  
Q. Zhou ◽  
H. Chipperfield ◽  
D. A. Melton ◽  
W. H. Wong
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Regulatory Network ◽  
Regulatory Network ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Regulatory

Melatonin affects hypoxia-stressed cardiomyocyte differentiation of mouse embryonic stem cells

2020 ◽  
Author(s):  
Eui-Bae Jeung ◽  
Jae-Hwan Lee ◽  
Park Seon Mi ◽  
Sunhwa Jeong ◽  
Yeong-Min Yoo
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cardiomyocyte Differentiation
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