Author response: Cell-type-specific control of basolateral amygdala neuronal circuits via entorhinal cortex-driven feedforward inhibition

The basolateral amygdala (BLA) plays a vital role in associating sensory stimuli with salient valence information. Excitatory principal neurons (PNs) undergo plastic changes to encode this association; however, local BLA inhibitory interneurons (INs) gate PN plasticity via feedforward inhibition (FFI). Despite literature implicating parvalbumin expressing (PV+) INs in FFI in cortex and hippocampus, prior anatomical experiments in BLA implicate somatostatin expressing (Sst+) INs. The lateral entorhinal cortex (LEC) projects to BLA where it drives FFI. In the present study, we explored the role of interneurons in this circuit. Using mice, we combined patch clamp electrophysiology, chemogenetics, unsupervised cluster analysis, and predictive modeling and found that a previously unreported subpopulation of fast-spiking Sst+ INs mediate LEC→BLA FFI.

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Cell-type-specific control elements of the lymphotropic papovavirus enhancer.

Journal of Virology ◽

10.1128/jvi.64.4.1657-1666.1990 ◽

1990 ◽

Vol 64 (4) ◽

pp. 1657-1666 ◽

Cited By ~ 10

Author(s):

J R Erselius ◽

B Jostes ◽

A K Hatzopoulos ◽

L Mosthaf ◽

P Gruss

Keyword(s):

Cell Type ◽

Cell Type Specific ◽

Lymphotropic Papovavirus ◽

Specific Control

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Cortical and thalamic inputs exert cell type‐specific feedforward inhibition on striatal GABAergic interneurons

Journal of Neuroscience Research ◽

10.1002/jnr.24444 ◽

2019 ◽

Cited By ~ 2

Author(s):

Maxime Assous ◽

James M. Tepper

Keyword(s):

Gabaergic Interneurons ◽

Cell Type ◽

Cell Type Specific ◽

Feedforward Inhibition

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L1CAM/Neuroglian controls the axon–axon interactions establishing layered and lobular mushroom body architecture

The Journal of Cell Biology ◽

10.1083/jcb.201407131 ◽

2015 ◽

Vol 208 (7) ◽

pp. 1003-1018 ◽

Cited By ~ 25

Author(s):

Dominique Siegenthaler ◽

Eva-Maria Enneking ◽

Eliza Moreno ◽

Jan Pielage

Keyword(s):

Cell Adhesion ◽

Mushroom Body ◽

Cell Adhesion Molecule ◽

Cluster Formation ◽

Neuronal Circuits ◽

Cell Type ◽

Cell Type Specific ◽

And Control ◽

Homophilic Adhesion

The establishment of neuronal circuits depends on the guidance of axons both along and in between axonal populations of different identity; however, the molecular principles controlling axon–axon interactions in vivo remain largely elusive. We demonstrate that the Drosophila melanogaster L1CAM homologue Neuroglian mediates adhesion between functionally distinct mushroom body axon populations to enforce and control appropriate projections into distinct axonal layers and lobes essential for olfactory learning and memory. We addressed the regulatory mechanisms controlling homophilic Neuroglian-mediated cell adhesion by analyzing targeted mutations of extra- and intracellular Neuroglian domains in combination with cell type–specific rescue assays in vivo. We demonstrate independent and cooperative domain requirements: intercalating growth depends on homophilic adhesion mediated by extracellular Ig domains. For functional cluster formation, intracellular Ankyrin2 association is sufficient on one side of the trans-axonal complex whereas Moesin association is likely required simultaneously in both interacting axonal populations. Together, our results provide novel mechanistic insights into cell adhesion molecule–mediated axon–axon interactions that enable precise assembly of complex neuronal circuits.

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