Twins from different fathers: A heteropaternal superfecundation case report in Colombia

Heteropaternal superfecundation is an extremely rare phenomenon that occurs when a second ova released during the same menstrual cycle is additionally fertilized by the sperm cells of a different man in separate sexual intercourse.In August, 2018, the Grupo de Genética de Poblaciones e Identificación at Universidad Nacional de Colombia received a request to establish the paternity of a pair of male twins with genetic markers. The following analyses were performed: amelogenin gene, autosomal short tandem repeat (STR), and Y-STR analyses by means of human identification commercial kits, paternity index, and the probability of paternity calculation and interpretation. A paternity index of 2.5134E+7 and a probability of paternity of 99.9999% for twin 2 were obtained while 14 out of 17 Y-chromosome markers and 14 out of 21 autosomal short tandem repeats were excluded for twin 1. The results indicated that the twins have different biological fathers.Although heteropaternal superfecundation is rarely observed among humans given its low frequency, in paternity disputes for dizygotic twins it is mandatory to demand the presence of the two twins in the testing to avoid wrong conclusions.

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Autosomal Short Tandem Repeats and anAluInsertion Polymorphism Are Detectable inCimex lectulariusL. (Hemiptera: Cimicidae) for 48 Hours after Human Blood Ingestion1

Journal of Agricultural and Urban Entomology ◽

10.3954/jaue15-03.1 ◽

2015 ◽

Vol 31 (1) ◽

pp. 62-69 ◽

Cited By ~ 1

Author(s):

Jennifer Raffaele ◽

Brittany McCarthy ◽

Ronald W. Raab ◽

Rajeev Vaidyanathan

Keyword(s):

Human Blood ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Autosomal Short Tandem Repeats ◽

Short Tandem

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Genetic diversity of 15 autosomal short tandem repeats loci using the AmpFLSTR®Identifiler™ kit in a Bhil Tribe Population from Gujarat state, India

Indian Journal of Human Genetics ◽

10.4103/0971-6866.142879 ◽

2014 ◽

Vol 20 (2) ◽

pp. 148 ◽

Cited By ~ 20

Author(s):

RameshR Chaudhari ◽

MS Dahiya

Keyword(s):

Genetic Diversity ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Gujarat State ◽

Autosomal Short Tandem Repeats ◽

Short Tandem

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Genetic polymorphism of 15 autosomal short tandem repeats in Baloch population of Pakistan

International Journal of Legal Medicine ◽

10.1007/s00414-018-1878-5 ◽

2018 ◽

Vol 133 (3) ◽

pp. 775-776 ◽

Cited By ~ 1

Author(s):

Aleena Ahmad Khan ◽

Rukhsana Perveen ◽

Nadeem Sheikh ◽

Babar Hilal Ahmad Abbasi ◽

Zunaira Batool ◽

...

Keyword(s):

Genetic Polymorphism ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Autosomal Short Tandem Repeats ◽

Short Tandem

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Contribution of ABO-Rhesus/Electrophoresis of hemoglobin methods and Short Tandem Repeats analysis in the determination of paternity in Burkina Faso, West Africa

10.21203/rs.3.rs-41454/v1 ◽

2020 ◽

Author(s):

Missa Millogo ◽

Serge Theophile Soubeiga ◽

Bapio Valerie Jean Telesphore Bazie ◽

Theodora Mahoukede Zohoncon ◽

Albert Theophane Yonli ◽

...

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

High Performance ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Repeat Analysis ◽

Paternity Index ◽

Genetic Analyzer ◽

Short Tandem

Abstract Background: the establishment of filiation by the current ABO, HLA, MNS, Kells and serum tests, pose a real reliability problem. It is then necessary to combine these methods with or to use high-performance methods such as microsatellite genetic analysis or short tandem repeats. This study aimed to compare the short tandem repeat technique with ABO/Rhesus system in combination with electrophoresis of hemoglobin. Methods: Fourteen (14) contested paternity trios were investigated. Blood samples were collected to determine blood groups using the Beth-Vincent method and the type of hemoglobin by electrophoresis. Blood spots on FTA paper were used for the analysis of 16 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, Amel) by capillary electrophoresis on the ABI 31310 Genetic Analyzer. The generated sequences were analyzed with GeneMapper® software version 3.2.1. The data were analyzed to determine the paternity index and the probability of paternity. Results: Of the fourteen (14) trios studied, ten (10) cases were probable inclusion, three (03) cases were exclusion and one (01) case was an undetermined paternity outcome with the ABO-Rhesus/ electrophoresis of hemoglobin system. While the analysis of genetic polymorphisms in DNA gave five (05) inclusions versus nine (09) exclusions of paternity. Of the 10 probable inclusion cases given by the ABO-Rhesus/Electrophoresis of hemoglobin system, only 05 cases (50%) were confirmed for inclusion by Short tandem repeat analysis. Conclusion: The analysis of short tandem repeat with sixteen genetic markers is more reliable in determining paternity than ABO-Rhesus/hemoglobin electrophoresis techniques.

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