The way of value of Correlation of genomic DNA microsatellite loci and live weight of Chukchi reindeer

2020 ◽  
pp. 27-32
Author(s):  
V. Dodokhov ◽  
N. Pavlova ◽  
T. Rumyantseva ◽  
L. Kalashnikova
Keyword(s):  
Microsatellite Markers ◽  
Agricultural Production ◽  
Live Weight ◽  
Biodiversity Loss ◽  
Tribal Community ◽  
High Germination ◽  
Wide Range ◽  
The Mean ◽  
High Level ◽  
Dna Microsatellite

The article presents the genetic characteristic of the Chukchi reindeer breed. The object of the study was of the Chukchi reindeer. In recent years, the number of reindeer of the Chukchi breed has declined sharply. Reduced reindeer numbers could lead to biodiversity loss. The Chukchi breed of deer has good meat qualities, has high germination viability and is adapted in adverse tundra conditions of Yakutia. Herding of the Chukchi breed of deer in Yakutia are engaged only in the Nizhnekolymsky district. There are four generic communities and the largest of which is the agricultural production cooperative of nomadic tribal community «Turvaurgin», which was chosen to assess the genetic processes of breed using microsatellite markers: Rt6, BMS1788, Rt 30, Rt1, Rt9, FCB193, Rt7, BMS745, C 143, Rt24, OheQ, C217, C32, NVHRT16, T40, C276. It was found that microsatellite markers have a wide range of alleles and generally have a high informative value for identifying of genetic differences between animals and groups of animal. The number of identified alleles is one of the indicators of the genetic diversity of the population. The total number of detected alleles was 127. The Chukchi breed of deer is characterized by a high level of heterozygosity, and the random crossing system prevails over inbreeding in the population. On average, there were 7.9 alleles (Na) per locus, and the mean number of effective alleles (Ne) was 4.1. The index of fixation averaged 0.001. The polymorphism index (PIC) ranged from 0.217 to 0.946, with an average of 0.695.

scholarly journals Microsatellites Cross-Species Amplification across Some African Cichlids

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Etienne Bezault ◽  
Xavier Rognon ◽  
Karim Gharbi ◽  
Jean-Francois Baroiller ◽  
Bernard Chevassus
Keyword(s):  
Microsatellite Markers ◽  
Oreochromis Niloticus ◽  
Molecular Genetic ◽  
Point Of View ◽  
Genetic Studies ◽  
African Cichlid ◽  
Wide Range ◽  
The Mean ◽  
High Level ◽  
African Cichlids

The transfer of the genomic resources developed in the Nile tilapia, Oreochromis niloticus, to other Tilapiines sensu lato and African cichlid would provide new possibilities to study this amazing group from genetics, ecology, evolution, aquaculture, and conservation point of view. We tested the cross-species amplification of 32 O. niloticus microsatellite markers in a panel of 15 species from 5 different African cichlid tribes: Oreochromines (Oreochromis, Sarotherodon), Boreotilapiines (Tilapia), Chromidotilapines, Hemichromines, and Haplochromines. Amplification was successfully observed for 29 markers (91%), with a frequency of polymorphic (P95) loci per species around 70%. The mean number of alleles per locus and species was 3.2 but varied from 3.7 within Oreochromis species to 1.6 within the nontilapia species. The high level of cross-species amplification and polymorphism of the microsatellite markers tested in this study provides powerful tools for a wide range of molecular genetic studies within tilapia species as well as for other African cichlids.

scholarly journals Polymorphic DNA microsatellite markers for forensic individual identification and parentage analyses of seven threatened species of parrots (family Psittacidae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2416 ◽  
Author(s):  
Catherine Jan ◽  
Luca Fumagalli
Keyword(s):  
Microsatellite Markers ◽  
Threatened Species ◽  
Captive Breeding ◽  
Genomic Library ◽  
Habitat Destruction ◽  
Individual Identification ◽  
Illegal Trade ◽  
The Mean ◽  
Dna Microsatellite ◽  
Dna Microsatellite Markers

The parrot family represents one of the bird group with the largest number of endangered species, as a result of habitat destruction and illegal trade. This illicit traffic involves the smuggling of eggs and animals, and the laundering through captive breeding facilities of wild-caught animals. Despite the huge potential of wildlife DNA forensics to determine with conclusive evidence illegal trade, current usage of DNA profiling approaches in parrots has been limited by the lack of suitable molecular markers specifically developed for the focal species and by low cross-species polymorphism. In this study, we isolated DNA microsatellite markers in seven parrot species threatened with extinction (Amazona brasiliensis,A. oratrix,A. pretrei,A. rhodocorytha,Anodorhynchus leari,Ara rubrogenysandPrimolius couloni). From an enriched genomic library followed by 454 pyrosequencing, we characterized a total of 106 polymorphic microsatellite markers (mostly tetranucleotides) in the seven species and tested them across an average number of 19 individuals per species. The mean number of alleles per species and across loci varied from 6.4 to 8.3, with the mean observed heterozygosities ranging from 0.65 to 0.84. Identity and parentage exclusion probabilities were highly discriminatory. The high variability displayed by these microsatellite loci demonstrates their potential utility to perform individual genotyping and parentage analyses, in order to develop a DNA testing framework to determine illegal traffic in these threatened species.

scholarly journals Genetic diversity in Brazilian tall coconut populations by microsatellite markers

2013 ◽  
Vol 13 (4) ◽  
pp. 356-362 ◽  
Author(s):  
Francisco Elias Ribeiro ◽  
Luc Baudouin ◽  
Patricia Lebrun ◽  
Lázaro José Chaves ◽  
Claudio Brondani ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Cape Verde ◽  
Coconut Palm ◽  
Different Populations ◽  
The Mean ◽  
High Level ◽  
Brazilian Populations

The tall coconut palm was introduced in Brazil in 1553, originating from the island of Cape Verde. The aim of the present study was to evaluate the genetic diversity of ten populations of Brazilian tall coconut by 13 microsatellite markers. Samples were collected from 195 individuals of 10 different populations. A total of 68 alleles were detected, with an average of 5.23 alleles per locus. The mean expected and observed heterozygosity value was 0.459 and 0.443, respectively. The number of alleles per population ranged from 36 to 48, with a mean of 40.9 alleles. We observed the formation of two groups, the first formed by the populations of Baía Formosa, Georgino Avelino and São José do Mipibu, and the second by the populations of Japoatã, Pacatuba and Praia do Forte. These results reveal a high level of genetic diversity in the Brazilian populations.

Growth and lifetime production of scottish blackface hill ewes in relation to the level of feeding during rearing

1972 ◽  
Vol 14 (3) ◽  
pp. 343-349 ◽  
Author(s):  
R. G. Gunn
Keyword(s):  
Live Weight ◽  
Long Term Effect ◽  
Nutritional Environment ◽  
The Mean ◽  
High Level ◽  
Mature Size ◽  
Female Sheep ◽  
Positive Effect ◽  
Different Levels

SUMMARYA study was made of the effects of different levels of nutrition between 3 and 6 months and between 6 and 12 months of age on subsequent growth to mature size, longevity and lifetime production of Scottish Blackface female sheep on a hill farm. Treatment differences between 3 and 6 months were small and resulted in only a 3 kg live-weight advantage for the animals receiving a high level of feeding. Treatment differences between 6 and 12 months were considerable and resulted in a mean 14 kg advantage for animals receiving a high level of feeding. Those animals remained significantly heavier until 42 months of age and significantly larger, as depicted by skeletal measurements, until 54 months of age.A high level of feeding between 3 and 6 months of age had no significant long-term effect on wool growth ewe survival or lifetime lamb production, whereas a high level of feeding between 6 and 12 months of age had a significant positive effect on the mean number of lambs born per ewe over five lamb crops. This effect was not maintained to weaning, due to an apparently greater lamb mortality. Treatment effects on ewe survival and on flock lifetime production, although considerable, were not statistically different.It is concluded that any advantages of improved feeding during rearing were largely lost through the inadequacy of the adult nutritional environment and only when the latter was not limiting would higher standards of rearing be justified.

Genetic differentiation in populations of the cestode Bothriocephalus acheilognathi (Cestoda, Pseudophyllidea) as revealed by eight microsatellite markers

Parasitology ◽  
2003 ◽  
Vol 126 (5) ◽  
pp. 493-501 ◽  
Author(s):  
H. Y. LUO ◽  
P. NIE ◽  
Y. A. ZHANG ◽  
W. J. YAO ◽  
G. T. WANG
Keyword(s):  
Genetic Structure ◽  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Polymorphic Information Content ◽  
Random Mating ◽  
Genetic Structure Of Populations ◽  
Bothriocephalus Acheilognathi ◽  
The Mean ◽  
High Level ◽  
Cross Fertilization

The genetic structure of populations of the fish cestode, Bothriocephalus acheilognathi collected from Bailianhe Reservoir (BLH), Changshou (CSH) and Liangzi (LZH) Lakes was investigated by using 8 microsatellite loci. A total of 108 adult worms were genotyped at each of the 8 loci. For the 3 populations, the mean number of alleles per locus ranged from 2·38 to 5·5, and the mean expected heterozygosity ranged from 0·432 to 0·559. The average polymorphic information content (PIC) was from 0·384 to 0·492. The significant Fis values indicated non-random mating within LZH and BLH populations. On the other hand, when samples were further classified into subpopulations at the level of host fish species, no or little heterozygote deficiency was detected at most loci, showing that cross-fertilization, predominantly, but not exclusively, must have occurred within the subpopulations. Microsatellite markers also revealed an unexpected high level of genetic differentiation, as measured by Rst and Nm values or by δu2 genetic distance among subpopulations from different hosts. Factors influencing the population genetic structure and the parasite host specificity are discussed.

The effect of suckling, management and season on sheep milk production as estimated by lamb growth

1962 ◽  
Vol 4 (2) ◽  
pp. 215-220 ◽  
Author(s):  
J. M. Doney ◽  
Joan Munro
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Live Weight ◽  
Seasonal Differences ◽  
Sheep Milk ◽  
Weight Gains ◽  
The Mean ◽  
High Level ◽  
The Hill ◽  
Lamb Growth

1. The mean daily live-weight gains of lambs from the 1956–59 flock records were used to estimate the milk yield of ewes during the first six weeks of lactation.2. The yield was affected by the number of lambs suckled but not by the number born. Ewes rearing twins on the hill gave a higher yield than those rearing singles (145%–155%). Good grazing raised the yield to twice that of ewes rearing singles on the hill.3. In 3 out of the 4 years the utilised yield was remarkably constant in the case of ewes rearing single lambs, but ewes rearing twins on the hill were more variable. Ewes rearing twins on low ground did not show any significant seasonal differences.4. From an experiment on 36 ewes it was confirmed that twin-rearing ewes produce more milk than single-rearing ewes in all environments. There was no difference between ewes rearing one lamb whether born as a single or as a twin. Leaving two lambs on the ewe for a few days to establish a high level of milk yield did not benefit the remaining lamb after its twin was removed.

scholarly journals A study on genetic diversity of bagrid catfish (Hemibagrus guttatus Lacepede, 1803) using microsatellite markers

2018 ◽  
Vol 16 (1) ◽  
pp. 59-65
Author(s):  
Bùi Hà My ◽  
Nguyễn Thị Hương ◽  
Nguyễn Hữu Đức ◽  
Trần Thị Thúy Hà
Keyword(s):  
Microsatellite Markers ◽  
Economic Value ◽  
Low Frequency ◽  
Wild Populations ◽  
Genetic Characteristics ◽  
Allele Number ◽  
Expected Heterozygosity ◽  
Artificial Breeding ◽  
The Mean ◽  
High Level

Bagrid catfish (Hemibagrus guttatus Lacepede, 1803) is a wild species of high economic value in Northern Vietnam. Artificial reproduction of bagrid catfish requires sources of quality fingerlings in terms of genetics. In fact, bagrid catfish is endangered due to overhunting. Until now, studying on bagrid catfish was mainly focused on biology characterictics and artificial breeding. In this study, three microsatellite markers were used to assess the genetic characteristics of four bagrid catfish populations (three wild populations collected in Tuyên Quang, Phu Tho, Ha Giang and a cultured population in Hai Duong). These markers were registed Genbwith the code of KJ873116, KJ873117 and NC 023976 for HM7, HM8 and SS1, respectively. All of the loci showed high level of polymorphism with 16 alleles in total which were 5 at locus HM7, 5 at locus HM8, and 6 at locus SS1. Total allele number in population collected in Ha Giang was higher than that of Tuyen Quang, Phu Tho and Hai Duong populations. The mean of number of observed alleles (Na = 3,83 ± 0,24) was higher than the mean number of effective alleles (Ne = 2,14 ± 0,13) and low frequency alleles (< 0,1) were observed in all loci. The value of the expected heterozygosity (He = 0,38- 0,63) was lower than that of the observed heterozygosity (Ho = 0,51-0,71). FIS value was low and the genetic differences between four populations was insignificant as FST < 0,05. The results provide useful information for breeding program and conservation of the bagrid catfish in the future.

scholarly journals Molecular Diversity Analysis of Jattal and Dera Din Panah Goat Breeds of Pakistan using Microsatellite Markers

2018 ◽  
Vol 69 (1) ◽  
pp. 791 ◽  
Author(s):  
T. HUSSAIN ◽  
M. SHAHEEN ◽  
M. E. BABAR ◽  
M. M. MUSTHAFA ◽  
A. NADEEM ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Resource ◽  
Polymorphic Information Content ◽  
Goat Breed ◽  
Expected Heterozygosity ◽  
Animal Genetic Resource ◽  
Wide Range ◽  
The Mean ◽  
Distinct Features

This paper evaluates the genetic diversity of goat breeds in Pakistan, a country blessed with a wide range of goats spread throughout with distinct features contributing to a rich Animal Genetic Resource. The genetic diversity of two goat breeds (Jattal and Dera Din Panah) was assessed with 25 animals representing the two breeds using 16 microsatellite markers. The mean observed and expected heterozygosity of both goat breed populations were observed as 0.83+0.21. The average number of observed alleles was 3.6+1.6 for all loci. The mean polymorphic information content for a goat breed was 0.45, indicating the usefulness of markers panel. Highest Nei’s standard genetic distance (Ds) value of 0.0612 was observed between Jattal and Dera Din Panah goats, and the mean Fst value was 0.013. The measures of genetic variation revealed a good scope for effective improvement, conservation, and designing national breeding policies, in future, for Pakistan goat breeds.

scholarly journals FACTORS INFLUENCING STUDENTS TENDENCY IN DOING ONLINE BUSINESS

2020 ◽  
Vol 2 (3) ◽  
pp. 01-11
Author(s):  
Iklima Husna Abdul Rahim ◽  
Pei Sung Toh
Keyword(s):  
Higher Education ◽  
Mean Value ◽  
The Internet ◽  
Additional Source ◽  
Business Economics ◽  
Factors Influencing ◽  
Wide Range ◽  
Online Business ◽  
The Mean ◽  
High Level

Nowadays, online business has become a phenomenon among traders. The business can grow globally with a wide range of applications that can be accessed through the internet. The advantages that exist today also can facilitate buyers in the purchasing process than purchase directly at the business premises where it can save buyers’ time. Hence, students in higher education institutions also are not lagging on the benefits of running an online business as an additional source of income. This is because an online business does not require high costs to involve and can be done even if the students have no business premises. Therefore, this study aimed to identify factors that influence students in doing online business. The research design is a quantitative study in questionnaires are used as an instrument with a 5-point Likert scale. The questionnaires were distributed online to the respondents through Google Form. Respondents involved 114 students in the entrepreneurship program at the Faculty of Business, Economics and Accounting, University Malaysia Sabah. Data were analyzed using Statistical Packages for Social Science (SPSS) version 21.0. Students’ tendency to do business online is measured by three factors, namely, source of encouragement, technology facilities and personal tendencies. The results show that the mean value of these three factors is at a high level. Hence, it can be concluded that the source of encouragement, technology facilities, and personal tendencies influence the students’ tendency to do business online.

scholarly journals Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite loci

2019 ◽  
Vol 23 (5) ◽  
pp. 569-574 ◽  
Author(s):  
L. A. Khrabrova ◽  
N. V. Blohina ◽  
O. I. Suleymanov ◽  
G. А. Rozhdestvenskaya ◽  
V. F. Pustovoy
Keyword(s):  
Genetic Differentiation ◽  
Microsatellite Loci ◽  
Dna Typing ◽  
Genetic Distances ◽  
Mean Value ◽  
Dna Microsatellites ◽  
Thoroughbred Horses ◽  
Wide Range ◽  
The Mean ◽  
Dna Microsatellite

The Thoroughbred (TB) horse is the best racehorse breed used in the racing industry. This breed has had a closed studbook for about 300 years. In Russia TB horses have been bred since the second half of the XVIII century. The modern Russian Stud Book register of TB horses is partially presented by stallions and broodmares imported from different countries. The genealogical structure of the breed is represented by 17 lines, among which the Northern Dancer line dominates (30.9 %). The study of features of different lines of TB was carried out on 17 loci of DNA microsatellites (VHL20, HTG4, AHT4, HMS7, HTG6, AHT5, HMS6, ASB23, ASB2, HTG10, HTG7, HMS3, HMS2, ASB17, LEX3, HMS1 and CA425) to assess genetic differentiation of the genealogical structure. The results of the DNA typing of 8091 Thoroughbred horses across microsatellite loci show that the gene pool of the domestic population is represented by 100 alleles typical of the breed. A comparative analysis of the genotypes of horses representing different lines indicates that they differ in the number of alleles (85–99), allele frequencies, the level of polymorphism Ae (2.93–3.48) and the degree of the observed heterozygosity Ho (0.653–0.739). The genetic distances between the lines varied in a wide range from 0.014 (Nasrullah – Northern Dancer) to 0.125 (Massine – Teddy). The correspondence to HWE was maintained in most lines, which is confirmed by the negative values of Fis. Cluster analysis demonstrated the correspondence of the obtained dendrogram of Nei’s genetic distances to its genealogical scheme of lines. Genetic differentiation of lines by index Fst varied in a range 0.005–0.073 at the mean value Fst = 0.024. The data indicate genetic differentiation of lines of TB at the STR markers and confirm the effectiveness of the system of linear breeding for the maintenance of interbreed biodiversity.

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