Microsatellite loci development and population genetics in Neotropical fish Curimata mivartii (Characiformes: Curimatidae)

The Curimatidae family plays an ecological role in the recycling and distribution of nutrients and constitutes a major food source for several commercially important fishes. Curimata mivartii, a member of this family, is considered a short-distance migratory species (≤100 km), categorized by the International Union for Conservation of Nature as a near threatened species, based on its declining population densities and habitat disturbance and fragmentation. Since population genetics and species-specific molecular tools remain unknown for all members of the Curimatidae family, this study developed a set of microsatellite loci and studied the population genetics of C. mivartii in the lower section of the Colombian Magdalena-Cauca basin. The results showed high levels of genetic diversity and evidence of gene flow even between locations separated over 350 km. This information provides a baseline for designing conservation and management programs for C.mivartii and constitutes the first study of population genetics in Curimatidae.

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Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci

Revista de Biología Tropical ◽

10.15517/rbt.v68i3.38405 ◽

2020 ◽

Vol 68 (3) ◽

Author(s):

Ricardo M. Landínez-García ◽

Edna J. Márquez

Keyword(s):

Population Genetics ◽

Microsatellite Loci ◽

Genomic Library ◽

International Union ◽

Genetic Population ◽

Genetic Diversity And Structure ◽

Polymorphic Microsatellite ◽

Species Specific ◽

Low Coverage ◽

Risk Categorization

Introduction: The freshwater fish Brycon henni (Characiformes: Bryconidae) is endemic to Colombia and currently considered as “least concern” according to the International Union for Conservation of Nature (IUCN). Objective: To develop microsatellite markers to examine population genetics in Brycon henni. Methods: Using a low-coverage sequenced genomic library, this study developed the first set of microsatellite loci to study the population genetics of this Neotropical species. These loci were used to evaluate the genetic diversity and structure of B. henni from three sites of the Magdalena-Cauca Basin (Colombia). Results: A set of twenty-one polymorphic microsatellite loci was highly informative and revealed that B. henni is evenly genetically structured between two tributaries of the Cauca River separated by only 30 km, a finding that indicates it conforms to reproductively isolated groups. Conclusions: We reported a set of twenty-one polymorphic microsatellite loci that was highly informative and allowed the detection of genetic structure at local and regional scales. This genetic population structure, concordant with that found in eight congeners, is relevant to estimate the B. henni risk categorization as well as for management, conservation, and restocking programs.

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Development of microsatellite loci and population genetics of the catfish Pimelodus yuma (Siluriformes: Pimelodidae)

Neotropical Ichthyology ◽

10.1590/1982-0224-2020-0114 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Cristhian Danilo Joya ◽

Ricardo Marcel Landínez-García ◽

Edna Judith Márquez

Keyword(s):

Genetic Diversity ◽

Population Genetics ◽

Population Structure ◽

Microsatellite Loci ◽

Anthropogenic Activities ◽

Lower Section ◽

Habitat Disturbances ◽

Genetic Groups ◽

Lack Of Information ◽

Species Specific

ABSTRACT Pimelodus yuma (formerly Pimelodus blochii) is a freshwater fish, endemic to the Colombian Magdalena-Cauca and Caribbean basins that experiences habitat disturbances resulting from anthropogenic activities. Due to the lack of information about the population genetics of this species, this study developed 14 species-specific microsatellite loci to assess the genetic diversity and population structure of samples from the lower section of the Cauca River. The studied species showed genetic diversity levels higher than the average values reported for Neotropical Siluriformes and significant inbreeding levels as was described for some congeners. Furthermore, P. yuma comprises two coexisting genetic groups that exhibit gene flow along the lower section of the Cauca River. This information constitutes a baseline for future monitoring of the genetic diversity and population structure in an anthropic influenced sector of the Magdalena-Cauca basin.

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Population genetics of the endangered catfish Pseudoplatystoma magdaleniatum (Siluriformes: Pimelodidae) based on species-specific microsatellite loci

Neotropical Ichthyology ◽

10.1590/1982-0224-2020-0120 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Kevin León García-Castro ◽

José David Rangel-Medrano ◽

Ricardo Marcel Landínez-García ◽

Edna Judith Márquez

Keyword(s):

Population Genetics ◽

Microsatellite Loci ◽

Anthropogenic Activities ◽

South American ◽

Important Species ◽

Large Size ◽

Genetic Diversity And Structure ◽

Commercially Important ◽

Species Specific ◽

Generation Sequencing

ABSTRACT The Neotropical catfish genus Pseudoplatystoma comprises eight species of large size, widely distributed in South American basins. The endangered species P. magdaleniatum is endemic to Magdalena basin (Colombia), experiences high fishing pressure and its population genetics is relatively unknown. To study the genetic status and structure of P. magdaleniatum, 25 species-specific polymorphic microsatellite loci were developed using next-generation sequencing and then tested in samples collected in the Magdalena-Cauca basin. Based on 15 of these loci, P. magdaleniatum showed a high number of alleles per locus (9-10), high values of observed (0.762-0.798) and expected (0.770-0.791) heterozygosities, recent reduction of population size and gene flow. These findings constitute a baseline to measure potential changes in genetic diversity and structure of this commercially important species in a basin undergoing high anthropogenic activities.

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