scholarly journals A new species of Scapholeberis Schoedler, 1858 (Anomopoda: Daphniidae: Scapholeberinae) from the Colombian Amazon basin highlighted by DNA barcodes and morphology

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9989
Author(s):  
Camilo Andrade-Sossa ◽  
Lorena Buitron-Caicedo ◽  
Manuel Elías-Gutiérrez

Background The Amazon basin is recognized as one of the most complex and species-rich freshwater environments globally. The diversity of zooplankton here remains unknown, with many species undescribed. Methods Here, we describe a new species of Scapholeberis Schoedler, 1858 (Cladocera: Anomopoda: Daphniidae: Scapholeberinae) from the Colombian Amazon Basin, collected with recently designed light traps. The description is based on detailed morphology (based on SEM and light microscopy) of parthenogenetic females, ephippial females, males, and molecular data based on the COI gene. Results Scapholeberis yahuarcaquensis n. sp. has a combination of characters present in Scapholeberis kingi Sars, 1888 and Scapholeberis armata freyi Dumont & Pensaert, 1983. These are a trilobate rostrum, with the middle lobe well developed with sides straight to relatively rounded, the presence of an elongated slit frontal head pore, a dorsal pore in the juncture of the cephalic shield and the valves, and a single denticulate membrane at the posterior rim of the valves, with stronger setae in the last third. The unique characters of the parthenogenetic females are ventral sucker with delicate triangles. Each has a filament-like projection in the lamellae’s inner side and an external section forming convex folds with denticle-like projections in the middle zone of the sucker-plate. There is a peculiar pitted sculpture in the ephippial females and a strong projection in the front of it. The male hook on the limb I with a blunt tip, a quirky lamella-like outgrow in the proximal side, and a paddle with well-developed spines scattered on its surface. The ventral sucker-lamellae in the male is much more developed than the female. The COI gene sequences showed an interspecific mean genetic divergence of 16.4% between S. yahuarcaquensis n. sp. and the closest species S. freyi from Mexico, supporting our results. A coalescence analysis and Barcode Index Number also support the new species based on the DNA sequences. New methods of collecting and integrative biology will give important support to recognize the fauna from the Amazon Basin, one of the most important sources of fresh water in the world that remains unknown in many respects.

Zootaxa ◽  
2021 ◽  
Vol 4985 (3) ◽  
Author(s):  
GYU-DONG CHANG ◽  
MIKHAIL POTAPOV ◽  
KYUNG-HWA PARK

A new species, Anurophorus hallaensis sp. nov. was collected from the withered leaves of Korean fir (Abies koreana Wilson), which is distributed at higher than 1,300 m in altitude on Mt. Hallasan (Jeju Island, South Korea). The morphology and two partial gene regions of this species are described herein. Anurophorus hallaensis sp. nov. showed the same formulas of macrochaetae and sensilla on thoracic segment II to abdominal segment IV as those exhibited by A. laricis Nicolet and A. palearcticus Potapov; however, this new species can be distinguished from them by the number of knobbed hairs on ventral side of legs. Partial DNA sequences of the mitochondrial Cytochrome c Oxidase subunit I (COI) gene and ribosomal DNA (28S rDNA) were used as DNA barcodes to distinguish between A. hallaensis sp. nov. and closely related congeners. The results of the present study indicate that the COI and rDNA are useful for species discrimination within the genus Anurophorus. An identification key to the Korean species of Anurophorus is provided.


Zootaxa ◽  
2020 ◽  
Vol 4896 (1) ◽  
pp. 85-95
Author(s):  
ROMAN V. YAKOVLEV ◽  
NAZAR A. SHAPOVAL ◽  
VADIM V. IVONIN ◽  
SVYATOSLAV A. KNYAZEV ◽  
GALINA N. KUFTINA ◽  
...  

We described a new cossid species, Dyspessa ulgen sp. nov. from the Tarbagatai and Altai Mountains and compared it to other taxa of Dyspessa reported from the region (D. tristis, D. saldaitisi, D. saissanica), as well as to morphologically similar D. ulula. The new species is most closely related to D. ulula but differs from the latter in the characteristics of the male genitalia, wing pattern, and molecular data (a 658 bp fragment of the mitochondrial COI gene). 


ZooKeys ◽  
2020 ◽  
Vol 959 ◽  
pp. 99-111
Author(s):  
Peter Huemer ◽  
Jae-Cheon Sohn

Eidophasia assmannisp. nov., a new species of Plutellidae from the alpine zone of Russian Altai Mountains, is described from diagnostic morphology and DNA barcodes. Male adult and genitalia are illustrated, whereas the female sex remains unknown. The species inhabits alpine scree with patchy herbaceous plants and is considered as possible endemic species of the Altai Mountains. An updated checklist of the 13 global Eidophasia Stephens, 1842 species is provided. The likely polyphyly of the genus is discussed from molecular data of the barcode region of the mt COI gene.


Zootaxa ◽  
2019 ◽  
Vol 4564 (2) ◽  
pp. 573 ◽  
Author(s):  
MING KAI TAN ◽  
JESSICA B. BAROGA-BARBECHO ◽  
SHERYL A. YAP

The taxonomy of Old World Trigonidiinae (sword-tailed crickets) is in need of major revision. This includes its type genus Trigonidium. Various authors have different opinions on what constitutes the subgenera and species of Trigonidium s. l. and this has led to considerable confusion and inconsistencies. Recent use of molecular data has helped make some progress in resolving some of these problems, but there are still limitations. In this paper, we aim to provide a baseline to resolve the confusion in the taxonomy of Trigonidium s. l. Firstly, we provide a literature review of the contentious taxa related to Trigonidium and also provide some recommendations on future work for Trigonidium s. l. Secondly, we reanalysed the gene tree (sensu Lu et al., 2018) using more DNA sequences from GenBank to test whether Trigonidium s. str. and Paratrigonidium should be considered separate genera or subgenera of Trigonidium s. l. However, the gene tree based on current data was not conclusive. Lastly, we describe a new species of Trigonidium s. str. from Siargao Island, Mindanao (Philippines): Trigonidium solis sp. nov. 


Phytotaxa ◽  
2017 ◽  
Vol 328 (2) ◽  
pp. 175
Author(s):  
YUAN YUAN ◽  
LU-LU SHEN

A new polypore, Rhodonia tianshanensis, collected from West Tianshan Nature Reserve in Xinjiang Autonomous Region (northwest China), is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by having resupinate basidiomata with an oblique tube layer, fusoid cystidioles in the hymenium, and cylindrical basidiospores. Based on multiple loci DNA sequences including the internal transcribed spacer (ITS), the large subunit (nLSU), and the second subunit of RNA polymerase II (RPB2) regions, our phylogeny strongly supported R. tianshanensis as a new species belonging to the genus Rhodonia.


Phytotaxa ◽  
2016 ◽  
Vol 283 (2) ◽  
pp. 143 ◽  
Author(s):  
KATEŘINA PROCHÁZKOVÁ ◽  
YVONNE NĚMCOVÁ ◽  
JIŘÍ NEUSTUPA

The trebouxiophycean genus Phyllosiphon contains unique green algae that thrive as endophytic parasites in the leaves of various members of the Araceae. The DNA sequences of the parasitic populations were originally acquired from infected leaves of subtropical Arisarum vulgare. However, several previous studies showed that the members of the Phyllosiphon clade also occur as free-living algae on various subaerial substrates across Europe. Phyllosiphon infection was also observed on the leaves of the temperate genus Arum, but no molecular data was available for these parasites. We recently found these parasites at a single sub-Mediterranean locality. These algae were genetically different from those previously obtained from Arisarum leaves on the basis of their 18S rDNA and rbcL gene sequences. In the present study, we describe this organism as a new species, Phyllosiphon ari. Phylogenetic differentiation of Phyllosiphon taxa, correlated with host specificity to different genera of the Araceae, suggests concerted host-pathogen co-evolution driving species diversification within this peculiar green algal lineage.


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