Phyllosiphon ari sp. nov. (Watanabea clade, Trebouxiophyceae), a new parasitic species isolated from leaves of Arum italicum (Araceae)

Phytotaxa ◽  
2016 ◽  
Vol 283 (2) ◽  
pp. 143 ◽  
Author(s):  
KATEŘINA PROCHÁZKOVÁ ◽  
YVONNE NĚMCOVÁ ◽  
JIŘÍ NEUSTUPA
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Molecular Data ◽  
Rbcl Gene ◽  
Free Living ◽  
Green Algal ◽  
Unique Green ◽  
Arum Italicum ◽  
A New Species ◽  
Rbcl Gene Sequences

The trebouxiophycean genus Phyllosiphon contains unique green algae that thrive as endophytic parasites in the leaves of various members of the Araceae. The DNA sequences of the parasitic populations were originally acquired from infected leaves of subtropical Arisarum vulgare. However, several previous studies showed that the members of the Phyllosiphon clade also occur as free-living algae on various subaerial substrates across Europe. Phyllosiphon infection was also observed on the leaves of the temperate genus Arum, but no molecular data was available for these parasites. We recently found these parasites at a single sub-Mediterranean locality. These algae were genetically different from those previously obtained from Arisarum leaves on the basis of their 18S rDNA and rbcL gene sequences. In the present study, we describe this organism as a new species, Phyllosiphon ari. Phylogenetic differentiation of Phyllosiphon taxa, correlated with host specificity to different genera of the Araceae, suggests concerted host-pathogen co-evolution driving species diversification within this peculiar green algal lineage.

Taxonomic notes on Trigonidium and related groups with one new species from the Philippines (Orthoptera: Trigonidiidae; Trigonidiinae)

Zootaxa ◽  
2019 ◽  
Vol 4564 (2) ◽  
pp. 573 ◽  
Author(s):  
MING KAI TAN ◽  
JESSICA B. BAROGA-BARBECHO ◽  
SHERYL A. YAP
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Gene Tree ◽  
Molecular Data ◽  
The Philippines ◽  
Current Data ◽  
Considerable Confusion ◽  
Future Work ◽  
One New Species ◽  
A New Species

The taxonomy of Old World Trigonidiinae (sword-tailed crickets) is in need of major revision. This includes its type genus Trigonidium. Various authors have different opinions on what constitutes the subgenera and species of Trigonidium s. l. and this has led to considerable confusion and inconsistencies. Recent use of molecular data has helped make some progress in resolving some of these problems, but there are still limitations. In this paper, we aim to provide a baseline to resolve the confusion in the taxonomy of Trigonidium s. l. Firstly, we provide a literature review of the contentious taxa related to Trigonidium and also provide some recommendations on future work for Trigonidium s. l. Secondly, we reanalysed the gene tree (sensu Lu et al., 2018) using more DNA sequences from GenBank to test whether Trigonidium s. str. and Paratrigonidium should be considered separate genera or subgenera of Trigonidium s. l. However, the gene tree based on current data was not conclusive. Lastly, we describe a new species of Trigonidium s. str. from Siargao Island, Mindanao (Philippines): Trigonidium solis sp. nov. 

A new species of Anurophorus (Collembola: Isotomidae) from South Korea, with notes on its molecular data

Zootaxa ◽  
2021 ◽  
Vol 4985 (3) ◽  
Author(s):  
GYU-DONG CHANG ◽  
MIKHAIL POTAPOV ◽  
KYUNG-HWA PARK
Keyword(s):  
New Species ◽  
South Korea ◽  
Dna Sequences ◽  
Ventral Side ◽  
Molecular Data ◽  
Coi Gene ◽  
Species Discrimination ◽  
Dna Barcodes ◽  
Abies Koreana ◽  
A New Species

A new species, Anurophorus hallaensis sp. nov. was collected from the withered leaves of Korean fir (Abies koreana Wilson), which is distributed at higher than 1,300 m in altitude on Mt. Hallasan (Jeju Island, South Korea). The morphology and two partial gene regions of this species are described herein. Anurophorus hallaensis sp. nov. showed the same formulas of macrochaetae and sensilla on thoracic segment II to abdominal segment IV as those exhibited by A. laricis Nicolet and A. palearcticus Potapov; however, this new species can be distinguished from them by the number of knobbed hairs on ventral side of legs. Partial DNA sequences of the mitochondrial Cytochrome c Oxidase subunit I (COI) gene and ribosomal DNA (28S rDNA) were used as DNA barcodes to distinguish between A. hallaensis sp. nov. and closely related congeners. The results of the present study indicate that the COI and rDNA are useful for species discrimination within the genus Anurophorus. An identification key to the Korean species of Anurophorus is provided.

Morphological characters and molecular data reveal a new species of Rhodonia (Polyporales, Basidiomycota) from China

Phytotaxa ◽  
2017 ◽  
Vol 328 (2) ◽  
pp. 175
Author(s):  
YUAN YUAN ◽  
LU-LU SHEN
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Dna Sequences ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Northwest China ◽  
Morphological Characters ◽  
Molecular Data ◽  
Molecular Evidence ◽  
A New Species

A new polypore, Rhodonia tianshanensis, collected from West Tianshan Nature Reserve in Xinjiang Autonomous Region (northwest China), is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by having resupinate basidiomata with an oblique tube layer, fusoid cystidioles in the hymenium, and cylindrical basidiospores. Based on multiple loci DNA sequences including the internal transcribed spacer (ITS), the large subunit (nLSU), and the second subunit of RNA polymerase II (RPB2) regions, our phylogeny strongly supported R. tianshanensis as a new species belonging to the genus Rhodonia.

Streblocladia (Rhodomelaceae) in New Zealand: recognition of a new species

Phytotaxa ◽  
2018 ◽  
Vol 374 (1) ◽  
pp. 41
Author(s):  
MYUNG SOOK KIM ◽  
BYEONGSEOK KIM ◽  
MI YEON YANG ◽  
ROBERTA D’ARCHINO ◽  
JUDY E. SUTHERLAND ◽  
...  
Keyword(s):  
New Species ◽  
New Zealand ◽  
Strong Support ◽  
Red Alga ◽  
Morphological Evidence ◽  
Rbcl Gene ◽  
Monophyletic Clade ◽  
Molecular Analyses ◽  
A New Species ◽  
Rbcl Gene Sequences

The genus Streblocladia was described by early European collectors based on S. neglecta, a marine red alga from southern New Zealand currently treated as a taxonomic synonym of Streblocladia glomerulata. In New Zealand, Streblocladia includes two species and has always been considered distinct. To analyse the phylogenetic relationships of this genus with other Polysiphonia sensu lato, we conducted a molecular-assisted investigation using plastid rbcL gene sequences of recent collections of Streblocladia from New Zealand, as well as morphological observations. The molecular analyses indicated that Streblocladia species form a monophyletic clade sister to P. rhododactyla with strong support. The Streblocladia clade is clearly separated from other species of Polysiphonia sensu lato. Species of Streblocladia are characterized by sympodially branched, corticated main axes, the absence of vegetative trichoblasts, and spermatangial branches that are unilaterally arranged and replace the whole trichoblast. As a result, the New Zealand endemic Polysiphonia rhododactyla is transferred to the genus Streblocladia on the basis of genetic and morphological evidence and compared with the two other species from New Zealand, S. glomerulata and S. muelleriana.

Review of the genus Cruziohyla (Anura: Phyllomedusidae), with description of a new species

Zootaxa ◽  
2018 ◽  
Vol 4450 (4) ◽  
pp. 401 ◽  
Author(s):  
ANDREW R. GRAY
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Dna Sequences ◽  
Integrated Approach ◽  
Molecular Data ◽  
Rrna Gene ◽  
Phylogenetic Information ◽  
Morphological And Molecular Data ◽  
A New Species ◽  
The 16S Rrna Gene

The presented work summarises new and existing phenotypic and phylogenetic information for the genus Cruziohyla. Data based on morphology and skin peptide profiling supports the identification of a separate new species. Specimens of Cruziohyla calcarifer (Boulenger, 1902) occurring in Ecuador, Colombia, two localities in Panama, and one in the south east Atlantic lowlands of Costa Rica, distinctly differ from those occurring along the Atlantic versant of Central America from Panama northwards through Costa Rica, Nicaragua, to Honduras. A new species—Cruziohyla sylviae sp. n.—(the type locality: Alto Colorado in Costa Rica)—is diagnosed and described using an integrated approach from morphological and molecular data. Phylogenetic analysis of DNA sequences of the 16S rRNA gene confirms the new species having equal minimum 6.2% genetic divergence from both true C. calcarifer and Cruziohyla craspedopus. 

scholarly journals A new species of Scapholeberis Schoedler, 1858 (Anomopoda: Daphniidae: Scapholeberinae) from the Colombian Amazon basin highlighted by DNA barcodes and morphology

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9989
Author(s):  
Camilo Andrade-Sossa ◽  
Lorena Buitron-Caicedo ◽  
Manuel Elías-Gutiérrez
Keyword(s):  
New Species ◽  
Ventral Sucker ◽  
Dna Sequences ◽  
Amazon Basin ◽  
Molecular Data ◽  
Middle Lobe ◽  
Coi Gene ◽  
Index Number ◽  
Colombian Amazon ◽  
A New Species

Background The Amazon basin is recognized as one of the most complex and species-rich freshwater environments globally. The diversity of zooplankton here remains unknown, with many species undescribed. Methods Here, we describe a new species of Scapholeberis Schoedler, 1858 (Cladocera: Anomopoda: Daphniidae: Scapholeberinae) from the Colombian Amazon Basin, collected with recently designed light traps. The description is based on detailed morphology (based on SEM and light microscopy) of parthenogenetic females, ephippial females, males, and molecular data based on the COI gene. Results Scapholeberis yahuarcaquensis n. sp. has a combination of characters present in Scapholeberis kingi Sars, 1888 and Scapholeberis armata freyi Dumont & Pensaert, 1983. These are a trilobate rostrum, with the middle lobe well developed with sides straight to relatively rounded, the presence of an elongated slit frontal head pore, a dorsal pore in the juncture of the cephalic shield and the valves, and a single denticulate membrane at the posterior rim of the valves, with stronger setae in the last third. The unique characters of the parthenogenetic females are ventral sucker with delicate triangles. Each has a filament-like projection in the lamellae’s inner side and an external section forming convex folds with denticle-like projections in the middle zone of the sucker-plate. There is a peculiar pitted sculpture in the ephippial females and a strong projection in the front of it. The male hook on the limb I with a blunt tip, a quirky lamella-like outgrow in the proximal side, and a paddle with well-developed spines scattered on its surface. The ventral sucker-lamellae in the male is much more developed than the female. The COI gene sequences showed an interspecific mean genetic divergence of 16.4% between S. yahuarcaquensis n. sp. and the closest species S. freyi from Mexico, supporting our results. A coalescence analysis and Barcode Index Number also support the new species based on the DNA sequences. New methods of collecting and integrative biology will give important support to recognize the fauna from the Amazon Basin, one of the most important sources of fresh water in the world that remains unknown in many respects.

A new pratylenchid nematode, Hoplotylus japonicus n. sp. (Tylenchina: Pratylenchidae) from the rhizosphere of Japanese maple, Acer palmatum (Thumb.)

Nematology ◽  
2020 ◽  
Vol 22 (10) ◽  
pp. 1131-1140
Author(s):  
Jianfeng Gu ◽  
Munawar Maria ◽  
Yiwu Fang ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Anterior Margin ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Dorsal Side ◽  
Lateral View ◽  
Molecular Phylogenetic ◽  
A New Species

Summary During quarantine inspections, a new species of Hoplotylus was detected from the rhizosphere of the Japanese maple, Acer palmatum, imported into China from Japan. Hoplotylus japonicus n. sp. is characterised by a stout 431-602 μm long body, well-developed 20.0-24.5 μm long stylet with tulip-shaped knobs having rounded sides in lateral view and a rounded anterior margin, excretory pore located slightly posterior to the hemizonid, usually empty, spermatheca, conical tail with a spike-like projection, which is distally smooth on the dorsal side, and males not found. It was morphologically compared with four currently known species of the genus, viz., H. femina, H. montanus, H. silvaticus, and H. sjacobi. In molecular phylogenetic analyses using 18S, 28S and internal transcribed spacer (ITS) ribosomal DNA sequences, the new species formed a clade with the only currently sequenced species of the genus, H. femina, in all phylogenies. This is the second species of the genus characterised by molecular data and the second species recovered from Japan.

Halenia coreana (Gentianaceae), a new species from Korea: Evidence from morphological and molecular data

Phytotaxa ◽  
2019 ◽  
Vol 403 (2) ◽  
pp. 86
Author(s):  
SU-MIN HAN ◽  
HYOSIG WON ◽  
CHAE EUN LIM
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Its Region ◽  
Molecular Data ◽  
Rbcl Gene ◽  
Species Status ◽  
Molecular Sequence Data ◽  
Molecular Sequence ◽  
Morphological And Molecular Data ◽  
A New Species

A new species of Halenia (Gentianaceae) from Korea, H. coreana S.M.Han, H.Won & C.E.Lim, is recognized based on morphological and molecular data, and its description and illustration are provided. It is distinct from H. corniculata in having long, narrower and incurved spurs and attenuated leaf apex. Molecular sequence data of nuclear ribosomal ITS region, nuclear XDH gene, and chloroplast rbcL gene also strongly support its species status.

Molecular and morphological evidence for a new species in the genus Sirodotia (Batrachospermales, Rhodophyta) from the State of Assam, India

Phytotaxa ◽  
2020 ◽  
Vol 437 (3) ◽  
pp. 121-134
Author(s):  
NATALIA L. ROSSIGNOLO ◽  
FARISHTA YASMIN ◽  
JOHN A. WEST ◽  
E.K. GANESAN ◽  
ORLANDO NECCHI JUNIOR
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Sequence Data ◽  
The State ◽  
Morphological Data ◽  
Morphological Evidence ◽  
Full Description ◽  
Rbcl Gene ◽  
A New Species

In India the genus Sirodotia has been documented solely on morphological data. A new species of Sirodotia (Sirodotia assamica sp. nov.) was found in two localities in the State of Assam, India. Phylogenetic relationships of the new species were inferred on the basis of DNA sequence data for the plastid rbcL gene and the barcode region of the mitochondrial COI-5P gene. Taxonomic affinities of the new species were determined by morphological analyses and a distinctive character was found for this species: spermatangia arranged in clusters. Although this character is also observed in S. huillensis, both species are genetically highly divergent (4.5–5.0% for rbcL and 9.6–10.1% for COI-5P). DNA sequences from Indian specimens formed a well-supported clade, sister to S. delicatula from Malaysia. DNA sequence divergence between S. assamica and S. delicatula varied from 2.5–2.7% for rbcL and COI-5P. Intraspecific divergence between the two sequences from India were low (0.4–0.5%). A full description and photographs of the new species are provided, as well as a comparison with morphologically similar and phylogenetically allied species reported from India and other Asian regions.

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