Acclimation of Teucrium polium Plants to Seasonal Variations by Alterations in the Activities of Antioxidant Enzymes and Protein Accumulation

Ceylon leadwort (Plumbago zeylanica) is ornamental plant known for its pharmacological properties arising from the abundant production of various secondary metabolites. It often grows in lead polluted areas. The aim of presented study was to evaluate the survival strategy of P. zeylanica to lead toxicity via photosynthetic apparatus acclimatization. Shoots of P. zeylanica were cultivated on media with different Pb concentrations (0.0, 0.05, and 0.1 g Pb∙l−1). After a four-week culture, the efficiency of the photosynthetic apparatus of plants was evaluated by Chl a fluorescence measurement, photosynthetic pigment, and Lhcb1, PsbA, PsbO, and RuBisCo protein accumulation, antioxidant enzymes activity, and chloroplast ultrastructure observation. Plants from lower Pb concentration revealed no changes in photosynthetic pigments content and light-harvesting complex (LHCII) size, as well as no limitation on the donor side of Photosystem II Reaction Centre (PSII RC). However, the activity and content of antioxidant enzymes indicated a high risk of limitation on the acceptor side of Photosystem I. In turn, plants from 0.1 g Pb∙l−1 showed a significant decrease in pigments content, LHCII size, the amount of active PSII RC, oxygen-evolving complex activity, and significant remodeling of chloroplast ultrastructure indicated limitation of PSII RC donor side. Obtained results indicate that P. zeylanica plants acclimate to lead toxicity by Pb accumulation in roots and, depending on Pb concentration, by adjusting their photosynthetic apparatus via the activation of alternative (cyclic and pseudocyclic) electron transport pathways.

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Role of hydrogen peroxide and the redox state of ascorbate in the induction of antioxidant enzymes in pea leaves under excess light stress

Functional Plant Biology ◽

10.1071/fp03246 ◽

2004 ◽

Vol 31 (4) ◽

pp. 359 ◽

Cited By ~ 39

Author(s):

Jose A. Hernández ◽

Carolina Escobar ◽

Gary Creissen ◽

Phil M. Mullineaux

Keyword(s):

Antioxidant Enzymes ◽

Redox State ◽

Light Stress ◽

Redox Status ◽

Protein Accumulation ◽

Photoinhibition Of Photosynthesis ◽

Transcript Accumulation ◽

Land Race ◽

Excess Light

In this work we used two different pea cultivars, JI281 is a semidomesticated land race of pea from Ethiopia whereas JI399 is a typical domesticated pea variety. Exposure of pea leaves to excess light (EL) for 1 h caused a reversible photoinhibition of photosynthesis as showed by changes in Fv / Fm. Although little difference existed between the two pea genotypes with respect to photoinhibition, after 60 min of EL the decline in Fv / Fm was higher in JI281 than in JI399 leaves. As a consequence of EL, H2O2 increased in both pea cultivars, whereas lipid peroxidation and protein oxidation slightly increased, although differences between cultivars were minimal. The redox state of ascorbate shifted towards its oxidized form under EL stress in both cultivars. Transcript levels of genes coding antioxidant enzymes varied with EL in both cultivars, but the response was more pronounced in JI399. The induction observed during EL was maintained or increased after the stress period, as occurred for cytGR and chlMDHAR. GR protein accumulation and activity correlated with the transcript accumulation in JI399, but not in JI288. In this work, a possible role for H2O2 and redox status of ascorbate in the photoxidative stress signalling is discussed.

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Seasonal variations in the activities of selected hepatic biotransformation and antioxidant enzymes in eelpout (Zoarces viviparus)

Comparative Biochemistry and Physiology Part C Pharmacology Toxicology and Endocrinology ◽

10.1016/s0742-8413(99)00074-2 ◽

1999 ◽

Vol 124 (3) ◽

pp. 271-279 ◽

Cited By ~ 6

Author(s):

Dan Ronisz ◽

D.G.Joakim Larsson ◽

Lars Förlin

Keyword(s):

Antioxidant Enzymes ◽

Seasonal Variations ◽

Zoarces Viviparus ◽

Hepatic Biotransformation

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Seasonal variation of flavonoids in Teucrium polium L. (Lamiaceae)

Macedonian Pharmaceutical Bulletin ◽

10.33320/maced.pharm.bull.2009.55.003 ◽

2009 ◽

Vol 55 ◽

pp. 33-40 ◽

Cited By ~ 1

Author(s):

Gjoshe Stefkov ◽

Marija Karapandzova ◽

Marina Stefova ◽

Svetlana Kulevanova

Keyword(s):

Seasonal Variation ◽

Seasonal Variations ◽

Plant Material ◽

Hplc Method ◽

Total Flavonoids ◽

Republic Of Macedonia ◽

Teucrium Polium ◽

Teucrium Polium L ◽

Ground Part

Тhe aim of the present study was identification of flavone aglycones and determination of the content of each and the content of total flavonoids as well as investigation of the eventual seasonal variations of flavonoids in Teucrium polium L. (Lamiaceae). The plant samples were collected at six different locations in Republic of Macedonia, during summer in 1999, 2000 and 2003. For determination of seasonal variations, the samples were collected in v. Koleshino, in 2004, each month during the whole season. Six flavone aglycones (luteolin, apigenin, diosmetin, cirsiliol, cirsimaritin and cirsilineol) were identified in the hydrolyzed extracts of the over ground part of Teucrium polium by HPLC method. The most abundant flavone was luteolin, followed by apigenin and cirsimaritin. Great seasonal variations were found in the content of each and in the content of total amount of flavonoids. The most abundant flavone during the whole season was luteolin with the highest content in May. The content of total flavonids was the highest in the period from May to July, which could be recommended as the most convenience period in the season for collecting of the plant material from Teucrium polium.

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