Optimization of Cultural Conditions for Production of Extracellular Polysaccharide by Halomonas xianhensis SUR308 Using Weighted Response Surface Methodology

2016 ◽  
Vol 8 (3) ◽  
pp. 1-11
Author(s):  
J Biswas ◽  
G Dutta ◽  
A Paul
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extracellular Polysaccharide ◽  
Cultural Conditions

Application of Response Surface Methodology for Optimization of Liquid Fermentation Medium for Polysaccharide Production by Tricholoma Mongolicum Imai

2012 ◽  
Vol 550-553 ◽  
pp. 1831-1836
Author(s):  
Xiao Tong Wu ◽  
Hai Xia Chen ◽  
Tian Xiang Han ◽  
Jing Bai ◽  
Ji Zhao ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Response Surface ◽  
Extracellular Polysaccharide ◽  
Fermentation Medium ◽  
Single Factor ◽  
Polysaccharide Production ◽  
Intracellular Polysaccharide ◽  
Optimal Medium

The fermentation medium of Tricholoma Mongolicum Imai for producing intracellular polysaccharide and extracellular polysaccharide was optimized by response surface methodology. The effects of carbon source, nitrogen source and mineral salt on polysaccharide production were examined by single factor experiments, glucose and yeast was found to be the best carbon source and nitrogen source. Based on this, the optimization of the Box-Benhnken design combined with response surface analysis showed that the optimal medium contained 40g/L glucose; 20g/L yeast extract; 0.40% KH2PO4; 0.40% MgSO4.The intracellular polysaccharide production and extracellular polysaccharide in medium reached 2.0512g/L and 2.394 g/L , respectively.

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