Optimization of cultural conditions using response surface methodology and modeling of indole-3-acetic acid production by Saccharothrix texasensis MB15

2022 ◽  
pp. 102271
Author(s):  
Abderrahmane Benadjila ◽  
Miyada Zamoum ◽  
Lamia Aouar ◽  
Abdelghani Zitouni ◽  
Yacine Goudjal
Keyword(s):  
Response Surface Methodology ◽  
Acetic Acid ◽  
Response Surface ◽  
Acid Production ◽  
Acetic Acid Production ◽  
Cultural Conditions ◽  
Indole 3 Acetic Acid

scholarly journals Optimization of fermentation conditions through response surface methodology for Indole-3-acetic acid production by Marine Sponge Callyspongia diffusa associated Pseudomonas fluorescens BCPBMS-1

2020 ◽  
Author(s):  
Vasanthabharathi Venkataraman ◽  
LakshmiNarayanan Ramasamy ◽  
Jayalakshmi Singaram
Keyword(s):  
Response Surface Methodology ◽  
Acetic Acid ◽  
Response Surface ◽  
Marine Sponge ◽  
Soya Bean ◽  
Gulf Of Mannar ◽  
Acetic Acid Production ◽  
Iaa Production ◽  
Callyspongia Diffusa ◽  
Indole 3 Acetic Acid

Abstract In the Present study we focused on the production and statistical optimization of media components for Indole-3 acetic acid (IAA) production from P.fluorescens BCPBMS-1, it was isolated from Marine sponge Callyspongia diffusa . This sponge was collected from Gulf of Mannar, Southeast coast of India. We optimized the low-cost agricultural residue based medium for IAA production. In our study Soya bean husk used as a substrate for Indole-3-acetic acid (IAA) production. Fermentation variables were selected based on the Plackett-Burman design and were optimized by response surface methodology. The maximum IAA concentration 1.7474 µg/ml was predicted in medium containing 3.1064% Soya bean husk, Yeast extract 0.8323%, Salinity 9.0765 ppt, pH 6.3108 temperature 27.5524℃, incubation time 64.7475hrs. These predicted values were also verified through experiments as a result we got 1.74 µg/ml. The excellent correlation observed between predicted and measured values of this statistical model hence, this method may recommend for the industrial purpose.

scholarly journals Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues

2005 ◽  
Vol 71 (3) ◽  
pp. 1169-1177 ◽  
Author(s):  
Olivier Vandeputte ◽  
Sevgi Öden ◽  
Adeline Mol ◽  
Danny Vereecke ◽  
Koen Goethals ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Acid Production ◽  
Infected Plant ◽  
Growth Media ◽  
Acid Biosynthesis ◽  
Gram Positive ◽  
Acetic Acid Production ◽  
Rhodococcus Fascians ◽  
Wide Range ◽  
Indole 3 Acetic Acid

ABSTRACT The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. The phytopathogen Rhodococcus fascians, a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. Phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. We show here for the first time that R. fascians produces and secretes the auxin indole-3-acetic acid. Interestingly, whereas noninfected-tobacco extracts have no effect, indole-3-acetic acid synthesis is highly induced in the presence of infected-tobacco extracts when tryptophan is not limiting. Indole-3-acetic acid production by a plasmid-free strain shows that the biosynthetic genes are located on the bacterial chromosome, although plasmid-encoded genes contribute to the kinetics and regulation of indole-3-acetic acid biosynthesis. The indole-3-acetic acid intermediates present in bacterial cells and secreted into the growth media show that the main biosynthetic route used by R. fascians is the indole-3-pyruvic acid pathway with a possible rate-limiting role for indole-3-ethanol. The relationship between indole-3-acetic acid production and the symptoms induced by R. fascians is discussed.

scholarly journals Screening of Indole-3-Acetic Acid (IAA) Productions by Endophytic Fusarium oxysporum Isolated from Phyllanthus niruri

1970 ◽  
Vol 7 (1) ◽  
pp. 56-59
Author(s):  
Abdul Raffi Junaidi ◽  
Mohamad Hasnul Bolhassan
Keyword(s):  
Acetic Acid ◽  
Fusarium Oxysporum ◽  
Endophytic Fungi ◽  
Acid Production ◽  
Indole Acetic Acid ◽  
Phyllanthus Niruri ◽  
Acetic Acid Production ◽  
Indole Acetic Acid Production ◽  
Indole 3 Acetic Acid

Ten newly isolated endophytic fungi from Phyllathus niruri Linn. were identified as Fusarium oxysporum. These isolates were screened out for their productivity of indole acetic acid (IAA) by the salkowski's method. Out of these isolates, two isolates showed high amount of indole acetic acid production, which were FO9 and FO10 with concentration of 23.52 μg/ml and 5.95 μg/ml, respectively.

Indole-3-acetamide-dependent auxin biosynthesis: A widely distributed way of indole-3-acetic acid production?

2010 ◽  
Vol 89 (12) ◽  
pp. 895-905 ◽  
Author(s):  
Thomas Lehmann ◽  
Maik Hoffmann ◽  
Mathias Hentrich ◽  
Stephan Pollmann
Keyword(s):  
Acetic Acid ◽  
Acid Production ◽  
Auxin Biosynthesis ◽  
Acetic Acid Production ◽  
Indole 3 Acetic Acid
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