scholarly journals Sugarcane Leaf Scald Disease in Côte d'Ivoire: Pathogenicity and Biocontrol of Xanthomonas albilineans Isolates

Author(s):  
N’guessan Aya Carine ◽  
Kouamé Konan Didier ◽  
Kassi Koffi Fernand Jean-martial ◽  
Kouamé Koffi Gaston ◽  
Konan Dawa Richard ◽  
...  

Aims: The present investigations describes bactericidal potential of essential oil for management of Xanthomonas albilineans (Ashby. 1929) Dowson 1943, pathogen responsible for sugarcane Leaf scald disease in Côte d'Ivoire. Study Design: the study was conducted at the Ferké 1 sugar mill and the Laboratory of Plant Physiology of Felix Houphouët BOIGNY University. Methodology: Diseased leaves specimens showing signs of X. albilineans presence were collected from sugarcane industrial plantations in Côte d'Ivoire. Pathogenicity was confirmed by observing Koch’s postulates under semi-controlled conditions. Asymptomatic leaves, obtained after 60 days of culture, were inoculated at three points by infiltration with bacterial suspension calibrated at 108 bacteria/ml. Apparition of small tan-brown necrotic lesions on the leaf blade, parallel to the veins and prolonged to the ends by a discolored vascular bundle confirmed that disease is caused by Xanthomonas albilineans. Three essential oils of aromatic plants whose antibacterial properties are recognized were used at 100; 300; 500; 1000 and 2000 ppm in comparison with Callicuivre (56% copper oxychloride) used as reference product. Results: The aqueous solutions based on these essential oils induced an inhibition zone of bacterial growth proportional to the dose applied and the incubation period. Thus, on day 5 of incubation, the essential oil solution of Ocimum gratissimum L. at 100 ppm induced an average inhibition zone diameter not significantly different from the one induced by the Cymbopogon citratus (DC.) Stapf solution at 300 ppm and the one of copper oxychloride at 1000 ppm. At 1000 ppm, Zingiber officinale Roscoe, Cymbopogon citratus (DC.) Stapf and Ocimum gratissimum L essential oils induced equivalent inhibition rates (6 mm) that were higher than that of the control (3.5 mm). Conclusion: sugarcane varieties cultivated at the Ferké 1 sugar mill complex, despite the selection for resistance are threatened by Leaf Scald Disease, which is significantly expressed on variety R585. Cymbopogon citratus (DC.) Stapf and Ocimum gratissimum L oils have the strongest antibacterial activity and may be useful to control Leaf Scald Disease.

Author(s):  
Coulibaly Klotioloma Ouattara Adama ◽  
Soro Sibirina Gogbe Françoise ◽  
N’guessan Walet Pierre Acka Kotaix ◽  
Kouame Norbert Tahi Mathias ◽  
Guiraud Brigitte Assi Maryse ◽  
...  

2018 ◽  
Vol 14 (21) ◽  
pp. 286 ◽  
Author(s):  
Koffi Christophe Kobenan ◽  
Vama Etienne Tia ◽  
Germain Elisabeth Cynthia Ochou ◽  
Malanno Kouakou ◽  
Kouadio Kra Norbert Bini ◽  
...  

The abusive use of synthetic pesticides in the phytosanitary protection of cotton in Côte d'Ivoire threatens the viability of the production system. The search for alternative control methods is therefore necessary. Phytosanitary strategies involving plant-based biopesticides have been explored. The insecticidal activity of essential oils of plant species of the genus Ocimum has been the subject of numerous investigations. The objective of this study is to compare the insecticidal potential of two species of the same genus Ocimum gratissimum and O. canum on a major cotton pest, the pink worm Pectinophora gossypiella. Adults of the insect were exposed in the laboratory to the toxic effect of different concentrations of these oils by topical application using a micro applicator. The lethal concentrations causing respectively 50 % (LC50) and 90% (LC90) death in the populations tested were determined for each of the essential oils. The oil extracted from O. gratissimum was the most toxic with LC50 and LC90 respective values of 1.01 % and 5.05 % as compared to O. canum (LC50 = 11.33 % and LC90= 30.04 %). The high toxicity of O. gratissimum may be explained by the presence of 24.57 % thymol and 37.79 % p-cimene in its chemical composition. The extract of O. gratissimum has shown in laboratory the highest potentiel insecticidal activity, it‘s field use can therefore be suggested for the control of this cotton pest.


2012 ◽  
Vol 04 (02) ◽  
pp. 106-111 ◽  
Author(s):  
Kanko Coffi ◽  
Kone Soleymane ◽  
Ramiarantsoa Harisolo ◽  
Tue Bi Balo ◽  
Chalchat Jean Claude ◽  
...  

2018 ◽  
Vol 33 (19) ◽  
pp. 2795-2800 ◽  
Author(s):  
Guy Blanchard Boué ◽  
Jean Brice Boti ◽  
Zanahi Félix Tonzibo ◽  
Mathieu Paoli ◽  
Ange Bighelli

2017 ◽  
Vol 12 (4) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Fatimata Nea ◽  
Evelyne Amenan Tanoh ◽  
Thierry Acafou Yapi ◽  
Gabriel Garcia ◽  
Felix Tomi ◽  
...  

The compositions of essential oil obtained from leaves, flowers and fruits of Lantana camara L. growing in wild at Côte d'Ivoire, were investigated by GC and 13C NMR. Thirty-seven compounds accounting for 88.3-94.5% of the oils have been identified. The leaf, flower and fruits of L. camara produced sesquiterpene-rich essential oils predominated by ( E)-β-caryophyllene and α-humulene, in addition to the important content of sabinene, β-phellandrene and thymol as monoterpenes. It was found the new chemical profiles of L. camara.


Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2400 ◽  
Author(s):  
Fatimata Nea ◽  
Didjour Albert Kambiré ◽  
Manon Genva ◽  
Evelyne Amenan Tanoh ◽  
Esse Leon Wognin ◽  
...  

This work aims to study the variations in the composition of Lantana camara leaf, flower, and stem essential oils over two years. L. camara organs were harvested in Bregbo (East Côte d’Ivoire) each month from June 2015 to June 2017. The essential oils were obtained by hydrodistillation and characterized by GC-MS and 13C NMR. Eighty-four compounds accounting for 84.4–99.1% of the essential oils have been identified. The essential oils hydrodistillated from L. camara are dominated by sesquiterpenes such as (E)-β-caryophyllene and α-humulene, which were found in all samples. Some monoterpenes such as thymol, sabinene, and α-pinene were also present. Statistical analysis (principal component analysis and clustering) revealed a high variability in essential oil composition between the different organs and also within the studied periods, as the thymol proportion was higher during flowering and fruiting months. In addition, the stem, flower, and fruit essential oils were more concentrated in thymol than the leaf essential oils. The proportions of (E)-β-caryophyllene and α-humulene were strictly inverted with the thymol proportion throughout the harvest period or vegetative cycle. The antioxidant, anti-inflammatory and insecticidal activities of leaves and flowers essential oils were also studied. Results showed that L. camara leaf and flower essential oils displayed high antioxidant, anti-inflammatory and insecticidal activities.


2018 ◽  
Vol 13 (2) ◽  
pp. 1934578X1801300
Author(s):  
Zana A. Ouattara ◽  
Nouho Sangaré ◽  
A. Janat Mamyrbekova-Bekro ◽  
Yves-Alain Békro ◽  
Pierre Tomi ◽  
...  

The essential oils isolated from aerial parts of Cassytha filiformis L. harvested in five locations of Côte d'Ivoire were analyzed by GC(RI), GC-MS and 13C-NMR. The oils were dominated by sesquiterpene derivatives and the content of the main components varied substantially from sample to sample: (E)-β-caryophyllene (1.5-34.9%), bicyclogermacrene (1.0-25.8%), α-humulene (0.5-22.0%), spathulenol (1.0-18.5%) and germacrene D (6.6-16.6%). Two compositions could be distinguished: (i) bicyclogermacrene, germacrene D, spathulenol (2/5 samples) and (ii), (E)-β-caryophyllene, α-humulene (3/5 samples).


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