scholarly journals Tuberous Root Initiation and Shoot Regeneration in Telfairia occidentalis Hook. F.

2017 ◽  
Vol 12 (03) ◽  
pp. 06-13
Author(s):  
Ayobola Moninuola Sakpere ◽  
Idowu Arinola Obisesan
2008 ◽  
Vol 102 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Amy Tsu Ku ◽  
Yi-Shiuan Huang ◽  
Yu-Shu Wang ◽  
Daifu Ma ◽  
Kai-Wun Yeh

2015 ◽  
Vol 7 (2) ◽  
pp. 47-52 ◽  
Author(s):  
S Mojumder ◽  
MD Hossain ◽  
MS Haque ◽  
KM Nasiruddin

The experiment was conducted to develop an efficient protocol for in vitro regeneration of mungbean (Vignaradiata) on the aspect of regeneration potentiality of two mungbean varieties (BINA mung 5 and BINA mung 7) as influenced by different combinations of growth regulators supplemented with MS medium. Cotyledon explant of both varieties was used for the present study. Data were collected for various characters of callus initiation, shoot regeneration and root proliferation. Initiation of callus (%) and required days for its initiation and weight of callus were influenced significantly due to the effect of varieties where BINA mung 5 produced more callus induction (40.36%) at minimum requiring time (18.27 days) including heavier sizes of callus (1.54 g) than BINA mung 7 when BINA mung 5 further recorded the longest root (2.92 cm) compare to BINA mung 7. Effect of treatments of the present study were significantly influenced the whole characters regarding callus culture, shoot regeneration and root proliferation. The highest percentage of callus (88.44%) within minimum time (12.53 days) including larger sizes callus (3.521 g) were produced in 1.0 mg L–1 BAP + 2.5 mg L–1 NAA among the treatments while the highest percentage of regenerated shoot (83.44%) at minimum requiring time (17.59 days) and more shoots (7.69 callus–1) were obtained in 1.0 mg L–1 BAP + 2.0 mg L– 1 NAA. Root induction (82.50%), number of roots plantlet–1 (8.469) with minimum requiring time for initiation (14.13 days) and root length (5.250 cm) were the highest in 0.2 mg L–1 IAA + 1.0 mg L–1 kinetin + 0.2 mg L–1 BAP. Incase of interaction, percentage of callus initiation (89.38 %) was the highest in BINA mung 5 treated by 1.0 mg L–1 BAP + 2.5 mg L–1 NAA at requiring minimum time (12.38 days) while same treatment produced the larger callus (3.581 g) among the interactions. The highest percentage (84.38%) and number (7.813 callus–1) of shoot with minimum requiring time (17.50 days) were found from BINA mung 5 treated by 1.0 mg L–1 BAP + 2.0 mg L–1 NAA. Similarly, the longest shoot (5.58 cm) was produced from the BINA mung 5 treated by 1.0 mg L–1 BAP + 2.0 mg L–1 NAA. However, root induction (%), roots plantlet–1, days required for root initiation and root length were statistically similar among the whole interaction treatments due to non significant variation. This result mentioned that the variety BINA mung 5 was better than BINA mung 7 for callus induction, shoot regeneration and root initiation while 1.0 mg L–1 BAP + 2.5 mg L–1 NAA, 1.0 mg L–1 BAP + 2.0 mg L–1 NAA and 0.2 mg L–1 IAA + 1.0 mg L–1 kinetin + 0.2 mg L–1 BAP supplemented with MS medium were the best combinations for better callusing, higher ability of shoot regeneration and root proliferation.DOI: http://dx.doi.org/10.3329/jesnr.v7i2.22203 J. Environ. Sci. & Natural Resources, 7(2): 47-52 2014


2021 ◽  
Author(s):  
Sneha Hasnu ◽  
Bhaben Tanti

In the present investigation, a micropropagation protocol has been developed for Vanilla borneensis Rolfe – a critically endangered orchid through multiple shoot regeneration. Through in vitro multiple shoot regeneration from both nodal and shoot tip explants, maximum (100%) shoot induction was observed. The minimum time required for shoot bud induction was observed from the shoot tip (5–7 days) on medium supplemented with BAP (4.44 mM) + KIN (2.32 mM) as compared to the nodal explants. Maximum multiple shoot regeneration was observed from nodal explants on the medium supplemented with BAP (4.44 mM) + TDZ (6.82 mM). However, maximum shoot length was observed on the medium supplemented with BAP (4.44 mM) + 15% CW and the number of nodes (5.27±0.33) per shoot after 90 days. Maximum (80-100%) of root initiation was observed in almost all the concentrations of NAA. The shortest time of root initiation was found on the medium supplemented with NAA (5.37 mM). Further, acclimatization period was found to be 15 days with 70% acclimatization while 60% of survivability was observed in the field condition. This efficient micropropagation method of V. borneensis could be successfully used for mass propagation as well as conservation of the critically endangered wild orchid.


1992 ◽  
Vol 118 (1) ◽  
pp. 63-70 ◽  
Author(s):  
M. P. Levett

SUMMARYThe effects of weed competition, harvest date and cultivar on sweet potato (Ipomoea batatas(L.) Lam.) yield and components of yield were investigated in three factorial experiments planted during the wet seasons of 1984 (Expt 1) and 1986 (Expts 2 and 3) in the tropical lowlands In Expts 1 and 2, the commencement of regular hand-weeding (every 14 days) was delayed for varying lengths of time following planting. In Expt 3, the duration of hand-weeding from planting onwards was varied. The main competing weed species following planting were Cyperus rotundus, C. brevifolius, Euphorbia geniculata and Portulaca oleracea. After canopy closure, E. hirta, Imperata cylindrica, Eleusine indica, Sida rhombifolia, Mollugo pentaphylla and Mimosa invisa were the predominant weeds to emerge and persist. Cultivars differed in their sensitivity to weed competition. In L431 both mean marketable root tuber weight and number of tubers/plant were severely reduced. However, in L44, tuber number was more sensitive than tuber weight: marketable tuber weight was significantly reduced only by prolonged weed competition (no weeding until 56 days after planting (DAP)). In Expts 1 and 2, delaying the commencement of weeding beyond 14 DAP significantly decreased vine weight, total yield and mean number of tubers/plant. In Expt 3, prolonging regular weeding until 14 DAP and longer significantly increased total and marketable tuber yield and mean number of tubers/plant.Effects of weed competition in the early part of the season during the first 28–42 DAP substantially reduced crop production in the later part of the growth cycle (after 110 DAP). This was probably due to a partial suppression of vine growth early on, which reduced tuberous root initiation and thus limited the number of root tubers that could develop in the later stages of crop growth. Hand-weeding during the period 28–42 DAP also appeared to have a detrimental effect on tuber initiation as a result of mechanical root disturbance at this sensitive period of root differentiation. There was also a trend towards lower yields when the crop was regularly hand-weeded after 56 DAP, probably due to disturbance of the vine canopy.The critical period for weed competition commenced at or before 14 DAP, possibly as early as 7 DAP, and did not continue beyond 56 DAP. In order to avoid weed competition and the detrimental mechanical effects of hand-weeding, an optimum hand-weeding programme for sweet potato in the tropical lowlands of will probably involve (i) keeping the crop weedfree for the first 14–21 DAP; (ii) no crop disturbance during the period of maximum tuberous root initiation (from c. 21–28 DAP until 42–56 DAP); (iii) clean weeding at c. 56 DAP; and (iv) minimal or no weeding after 56 DAP. Weeding during the period 7–14 DAP is particularly important.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.


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