Chromosome-Level Genome Assembly Provides New Insights into Genome Evolution and Tuberous Root Formation of Potentilla anserina

Potentilla anserina is a perennial stoloniferous plant with edible tuberous roots in Rosaceae, served as important food and medicine sources for Tibetans in the Qinghai-Tibetan Plateau (QTP), China, over thousands of years. However, a lack of genome information hindered the genetic study. Here, we presented a chromosome-level genome assembly using single-molecule long-read sequencing, and the Hi-C technique. The assembled genome was 454.28 Mb, containing 14 chromosomes, with contig N50 of 2.14 Mb. A total of 46,495 protein-coding genes, 169.74 Mb repeat regions, and 31.76 Kb non-coding RNA were predicted. P. anserina diverged from Potentilla micrantha ∼28.52 million years ago (Mya). Furthermore, P. anserina underwent a recent tetraploidization ∼6.4 Mya. The species-specific genes were enriched in Starch and sucrose metabolism and Galactose metabolism pathways. We identified the sub-genome structures of P. anserina, with A sub-genome was larger than B sub-genome and closer to P. micrantha phylogenetically. Despite lacking significant genome-wide expression dominance, the A sub-genome had higher homoeologous gene expression in shoot apical meristem, flower and tuberous root. The resistance genes was contracted in P. anserina genome. Key genes involved in starch biosynthesis were expanded and highly expressed in tuberous roots, which probably drives the tuber formation. The genomics and transcriptomics data generated in this study advance our understanding of the genomic landscape of P. anserina, and will accelerate genetic studies and breeding programs.

Mitigation of CaCO3 Influences on Ipomoea batatas Plants Using Bacillus megaterium BSM 2894 var.

Under Egyptian soil conditions, when phosphorus fertilizers were applied to the soil, it gets fixed and converts to unavailable form, leading to low solubility for the plant. This study were fulfilled on sweet potato (cv. Beauregard) under undesirable soil properties (CaCO3 10.8 vs 11.3%) using Bacillus megaterium DSM 2894 strain under different five mono calcium phosphate (CSP) levels [(69(CSP20); 138(CSP40); 207 (CSP60); 276 (CSP80) and 345 ((CSP100) kg ha-1 of calcium superphosphate (CSP)] to arise the potential efficiency of some nutrients uptake and decease the applied total amount of CSP in 2019 and 2020 seasons. The results mentioned that highest values were obtained by inoculated plants with DSM2894 strain under 20, 60 and 100% of CSP for all studied nutrients content in both seasons, except Mn content in the 2019. Also, inoculated plants with DSM2894 under previous treatments for all tuberous root nutrients content, except Fe and Zn contents in both seasons, in addition protein and anti-radical power and total yield. Statistically, leaf nutrients uptake and tuberous root content were highly significant affected by DSM and CSP combination. Appling of phosphorus fertilizer with DSM2894 mixture was gave the best values as compared with phosphorus fertilizer or DSM2894, individually.

Growth and yield of beet irrigated with saline water in different types of vegetable mulching

ABSTRACT Vegetal mulching can mitigate the harmful effects of salts present in irrigation water. This study aimed to evaluate the effect of irrigation water salinity and mulching on the growth and yield of beet crops. The experiment was conducted in a greenhouse at Redenção, Ceará State. The experimental design was completely randomized in a 2 × 4 factorial scheme, with five replicates, composed of two levels of electrical conductivity of irrigation water - ECw: tap water - 0.3 dS m-1 and saline solution - 5.8 dS m-1; and four types of mulching (rice hulls, carnauba bagana, crop residues, and a control treatment without soil cover). The use of vegetal mulching, mitigated the effects of irrigation with saline water on the variables plant height, leaf area, stem diameter, and tuberous root. The increase of the salt concentration in irrigation water negatively affected the number of leaves, length of the tuberous root, and yield. But, it increased the soluble solids in the beet crop.

Genome-Wide Identification and Expression Analysis of the MADS-Box Gene Family in Sweet Potato [Ipomoea batatas (L.) Lam]

MADS-box gene, one of the largest transcription factor families in plants, is a class of transcription factors widely present in eukaryotes. It plays an important role in plant growth and development and participates in the growth and development of flowers and fruits. Sweet potato is the seventh most important food crop in the world. Its tuberous roots, stems, and leaves contain a large number of proteins, lipids, carotenoids, anthocyanins, conjugated phenolic acids, and minerals, which have high edible, forage, and medicinal value, and is also an important energy crop. At present, MADS-box genes in sweet potato have rarely been reported, and there has been no study on the genome-wide identification and classification of MADS-box genes in Ipomoea batatas. This study provided the first comprehensive analysis of sweet potato MADS-box genes. We identified 95 MADS-box genes, analyzed the structure and protein of sweet potato MADS-box genes, and categorized them based on phylogenetic analysis with Arabidopsis MADS-box proteins. Chromosomal localization indicated an unequal number of MADS-box genes in all 14 chromosomes except LG3, with more than 10 MADS-box genes located on chromosomes LG7, LG11, and LG15. The MADS domain and core motifs of the sweet potato MADS-box genes were identified by motif analysis. We identified 19 MADS-box genes with collinear relationships and analyzed duplication events. Cis-acting elements, such as light-responsive, auxin-responsive, drought-inducible, and MeJA-responsive elements, were found in the promoter region of the MADS-box genes in sweet potato, which further indicates the basis of MADS-box gene regulation in response to environmental changes and hormones. RNA-seq suggested that sweet potato MADS-box genes exhibit tissue-specific expression patterns, with 34 genes highly expressed in sweet potato flowers and fruits, and 19 genes highly expressed in the tuberous root, pencil root, or fibrous root. qRT-PCR again validated the expression levels of the 10 genes and found that IbMADS1, IbMADS18, IbMADS19, IbMADS79, and IbMADS90 were highly expressed in the tuberous root or fibrous root, and IbMADS18, IbMADS31, and IbMADS83 were highly expressed in the fruit. In this study, the molecular basis of MADS-box genes of sweet potato was analyzed from various angles. The effects of MADS-box genes on the growth and development of sweet potato were investigated, which may provide a certain theoretical basis for molecular breeding of sweet potato.

PRELIMINARY COMPARATIVE PHARMACOGNOSTIC STUDY OF GENUINE AND ADULTERANT RAW MATERIAL IN ATIVISHA (ACONITUMHETEROPHYLLUM WALL. EX ROYLE)

Ativisha consists of the dried tuberous root of the aconitum heterophyllum wall. ex royle of ranunculaceae family. a perennial herb native and endemic to western himalayas. it is popular drug that is extensively used in ayurvedic pediatric medicine, specially used in respiratory disorders, fever, diarrhoea and vomiting in children. the fact that the suppliers of crude herbal materials are mostly traders having limited knowledge of their true identity. due to unavailability and high cost, they use their substitutes and adulterants in order to get more and more benefits. children's being more vulnerable, special care has to be taken in selecting the drugs. this paper includes preliminary pharmacognostical but very important aspects to ensure the standard and quality assurance of the raw drug of ativisha. preliminary comparative pharmacognostic study of genuine and adulterant raw material in ativisha. here the sample genuine ativisha with both small and big sized starch grains and taste is bitter followed by salivation in the mouth it may official ativisha. where the other sample with big starch grains with sweet taste may be an adulterated market sample sold under the name of ativisha.

Tuber and Tuberous Root Development

Root and tuber crops have been an important part of human nutrition since the early days of humanity, providing us with essential carbohydrates, proteins, and vitamins. Today, they are especially important in tropical and subtropical regions of the world, where they help to feed an ever-growing population. Early induction and storage organ size are important agricultural traits, as they determine yield over time. During potato tuberization, environmental and metabolic status are sensed, ensuring proper timing of tuberization mediated by phloem-mobile signals. Coordinated cellular restructuring and expansion growth, as well as controlled storage metabolism in the tuber, are executed. This review summarizes our current understanding of potato tuber development and highlights similarities and differences to important tuberous root crop species like sweetpotato and cassava. Finally, we point out knowledge gaps that need to be filled before a complete picture of storage organ development can emerge. Expected final online publication date for the Annual Review of Plant Biology, Volume 72 is May 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Correction to: The acrid raphides in tuberous root of Pinellia ternata have lipophilic character and are specifically denatured by ginger extract

A correction to this paper has been published: https://doi.org/10.1007/s11418-021-01501-5

CLOD STRUCTURE AND THE QUALITY OF MORINGA SEEDLINGS (Moringa oleifera LAM.) GROWN IN COMMERCIAL SUBSTRATE AND IN ORGANIC COMPOSTS

ABSTRACT The cultivation of tuberous-root species such as Moringa oleifera Lam. (moringa) requires well-dimensioned containers and the use of appropriate substrates, since seedlings will be removed from the container before their planting. Sugarcane bagasse, urban waste compost (compost), and vermicompost are promising wastes for substrate composition. The present study aims to assess the quality of moringa grown in substrate produced from sugarcane bagasse associated with compost or vermicompost in different-volume tubes. The study followed a randomized blocks design, at 2x7 factorial arrangement, namely: tubes’ volumes (50 and 240 mL) x seven substrates (commercial substrate; sugarcane bagasse associated with urban waste compost at three different ratios (1:3; 1:1 and 3:1) and sugarcane bagasse associated with vermicompost at ratios 1:3; 1:1 and 3:1). In conclusion, the 240 mL container was the most appropriate one for moringa seedlings’ production. Substrates presenting higher organic compost ratios led to greater shoot and tuberous root growth and to greater nitrogen-use accumulation and efficiency, which was equivalent to that of the commercial substrate. Higher sugarcane bagasse rates in substrate composition made it easier to remove the seedlings from the tubes and led to better physical quality of the clod after seedling removal from the tubes.

Highly dynamic, coordinated, and stage-specific profiles are revealed by a multi-omics integrative analysis during tuberous root development in cassava

Cassava (Manihot esculenta) is an important starchy root crop that provides food for millions of people worldwide, but little is known about the regulation of the development of its tuberous root at the multi-omics level. In this study, the transcriptome, proteome, and metabolome were examined in parallel at seven time-points during the development of the tuberous root from the early to late stages of its growth. Overall, highly dynamic and stage-specific changes in the expression of genes/proteins were observed during development. Cell wall and auxin genes, which were regulated exclusively at the transcriptomic level, mainly functioned during the early stages. Starch biosynthesis, which was controlled at both the transcriptomic and proteomic levels, was mainly activated in the early stages and was greatly restricted during the late stages. Two main branches of lignin biosynthesis, coniferyl alcohol and sinapyl alcohol, also functioned during the early stages of development at both the transcriptomic and proteomic levels. Metabolomic analysis further supported the stage-specific roles of particular genes/proteins. Metabolites related to lignin and flavonoid biosynthesis showed high abundance during the early stages, those related to lipids exhibited high abundance at both the early and middle stages, while those related to amino acids were highly accumulated during the late stages. Our findings provide a comprehensive resource for broadening our understanding of tuberous root development and will facilitate future genetic improvement of cassava.