Exploratory X-Ray Monitoring of Luminous Radio-quiet Quasars at High Redshift: Extended Time-series Analyses and Stacked Imaging Spectroscopy

We present three new Chandra X-ray epochs along with new ground-based optical–UV observations as the third installment in a time-series analysis of four high-redshift (z ≈ 4.1–4.4) radio-quiet quasars. In total, we present nine epochs for these sources with rest-frame temporal baselines of ∼1300–2000 days. We utilize the X-ray data to determine basic variability properties, as well as produce mean spectra and stacked images based on effective exposure times of ∼40–70 ks per source. We perform time-series analyses in the soft and hard bands, separately, and compare variability properties to those of sources at lower redshifts and luminosities. The magnitude of X-ray variability of our sources remains consistent with or lower than that of similar sources at lower redshifts, in agreement with the variability–luminosity anticorrelation. The mean power-law photon indices in the stacked Chandra spectra of our sources are consistent with the values measured from their archival XMM-Newton spectra separated by about 3 yr in the rest frame. Along with the X-ray observations, we provide near-simultaneous optical monitoring of the sources in the optical–UV regime. The overall variability in the optical-to-X-ray spectral slope is consistent with sources at lower redshifts, and the optical–UV observations display mild variability on monthly timescales.

Optical Variability of a Newly Discovered Blazar Sample from the BZCAT Catalog

In an optical monitoring program to characterize the variability properties of blazars, we observed 10 sources from the Roma-BZCAT catalog for 26 nights in V and R bands during 2014 October to 2015 June with two telescopes located in India. The sample includes mainly newly discovered BL Lacertae objects (BL Lacs) for which the redshift of some sources is not yet known. We present the results of flux and color variations of the sample on intraday and short timescales obtained by using the power-enhanced F-test and the nested-ANOVA tests, along with their spectral behavior. We find significant intraday variability in the single flat-spectrum radio quasar in our sample, having an amplitude of variation ∼12%. Although a few of the BL Lacs showed probable variation in some nights, none of them passed the variability tests at 99.9% significance level. We find that 78% of the sample showed significant negative color–magnitude correlations, i.e., a redder-when-brighter spectral evolution. Those that do not show strong or clear chromatism predominantly exhibit a redder-when-brighter trend. Unlike on hourly timescales, the high-synchrotron-peaked blazars in the sample (BZGJ0656+4237, BZGJ0152+0147, and BZBJ1728+5013) show strong flux variation on timescales of days to months, where again we detect a decreasing trend of the spectral slope with brightness. We observe a global steepening of the optical spectrum with increasing flux on the intranight timescale for the entire blazar sample. The nonvariability in the BL Lacs in our sample might be caused by the distinct contribution from the disk as well as from other components in the studied energy range.

An Optical Probe for Real-Time Monitoring of Self-Replicator Emergence and Distinguishing between Replicators

Self-replicating systems play an important role in research on the synthesis and origin of life. Monitoring of these systems has mostly relied on techniques such as NMR or chromatography, which are limited in throughput and demanding when monitoring replication in real time. To circumvent these problems, we now developed a pattern-generating fluorescent molecular probe (an ID-probe) capable of discriminating replicators of different chemical composition and monitoring the process of replicator formation in real time, giving distinct signatures for starting materials, intermediates and final products. Optical monitoring of replicators dramatically reduces the analysis time and sample quantities compared to most currently used methods and opens the door for future high-throughput experimentation in protocell environments.

Postsynaptic synucleins mediate vesicular exocytosis of endocannabinoids

Two seemingly unrelated questions have long motivated studies in neuroscience: How are endocannabinoids, among the most powerful modulators of synaptic transmission, released from neurons? What are the physiological functions of synucleins, key contributors to Parkinson’s Disease? Here, we report an unexpected convergence of these two questions: Endocannabinoids are released via vesicular exocytosis from postsynaptic neurons by a synuclein-dependent mechanism. Specifically, we find that deletion of all synucleins selectively blocks all endocannabinoid-dependent synaptic plasticity; this block is reversed by postsynaptic expression of wildtype but not of mutant α-synuclein. Loading postsynaptic neurons with endocannabinoids via patch-pipette dialysis suppressed presynaptic neurotransmitter release in wildtype but not in synuclein-deficient neurons, suggesting that the synuclein deletion blocks endocannabinoid release. Direct optical monitoring of endocannabinoid release confirmed the requirement of synucleins. Given the role of synucleins in vesicular exocytosis, the requirement for synucleins in endocannabinoid release indicates that endocannabinoids are secreted via exocytosis. Consistent with this hypothesis, postsynaptic expression of tetanus-toxin light chain, which cleaves synaptobrevin SNAREs, also blocked endocannabinoid-dependent plasticity and release. The unexpected finding that endocannabinoids are released via synuclein-dependent exocytosis assigns a function to synucleins and resolves a longstanding puzzle of how neurons release endocannabinoids to induce synaptic plasticity.

Postsynaptic synucleins mediate vesicular exocytosis of endocannabinoids

Two seemingly unrelated questions have long motivated studies in neuroscience: How are endocannabinoids, among the most powerful modulators of synaptic transmission, released from neurons? What are the physiological functions of synucleins, key contributors to Parkinson's Disease? Here, we report an unexpected convergence of these two questions: Endocannabinoids are released via vesicular exocytosis from postsynaptic neurons by a synuclein-dependent mechanism. Specifically, we find that deletion of all synucleins selectively blocks all endocannabinoid-dependent synaptic plasticity; this block is reversed by postsynaptic expression of wildtype but not of mutant α-synuclein. Loading postsynaptic neurons with endocannabinoids via patch-pipette dialysis suppressed presynaptic neurotransmitter release in wildtype but not in synuclein-deficient neurons, suggesting that the synuclein deletion blocks endocannabinoid release. Direct optical monitoring of endocannabinoid release confirmed the requirement of synucleins. Given the role of synucleins in vesicular exocytosis, the requirement for synucleins in endocannabinoid release indicates that endocannabinoids are secreted via exocytosis. Consistent with this hypothesis, postsynaptic expression of tetanus-toxin light chain, which cleaves synaptobrevin SNAREs, also blocked endocannabinoid-dependent plasticity and release. The unexpected finding that endocannabinoids are released via synuclein-dependent exocytosis assigns a function to synucleins and resolves a longstanding puzzle of how neurons release endocannabinoids to induce synaptic plasticity.

Coherent radio emission from a population of RS Canum Venaticorum systems

Coherent radio emission from stars can be used to constrain fundamental coronal plasma parameters, such as plasma density and magnetic field strength. It is also a probe of chromospheric and magnetospheric acceleration mechanisms. Close stellar binaries, such as RS Canum Venaticorum (RS CVn) systems, are particularly interesting as their heightened level of chromospheric activity and possible direct magnetic interaction make them a unique laboratory to study coronal and magnetospheric acceleration mechanisms. RS CVn binaries are known to be radio-bright but coherent radio emission has only conclusively been detected previously in one system. Here, we present a population of 14 coherent radio emitting RS CVn systems. We identified the population in the ongoing LOFAR Two Metre Sky Survey as circularly polarised sources at 144 MHz that are astrometrically associated with known RS CVn binaries. We show that the observed emission is powered by the electron cyclotron maser instability. We use numerical calculations of the maser’s beaming geometry to argue that the commonly invoked ‘loss-cone’ maser cannot generate the necessary brightness temperature in some of our detections and that a more efficient instability, such as the shell or horseshoe maser, must be invoked. Such maser configurations are known to operate in planetary magnetospheres. We also outline two acceleration mechanisms that could produce coherent radio emission, one where the acceleration occurs in the chromosphere and one where the acceleration is due to an electrodynamic interaction between the stars. We propose radio and optical monitoring observations that can differentiate between these two mechanisms.