A space–time Bayesian hierarchical modeling framework for projection of seasonal maximum streamflow

Timely projections of seasonal streamflow extremes can be useful for the early implementation of annual flood risk adaptation strategies. However, predicting seasonal extremes is challenging, particularly under nonstationary conditions and if extremes are correlated in space. The goal of this study is to implement a space–time model for the projection of seasonal streamflow extremes that considers the nonstationarity (interannual variability) and spatiotemporal dependence of high flows. We develop a space–time model to project seasonal streamflow extremes for several lead times up to 2 months, using a Bayesian hierarchical modeling (BHM) framework. This model is based on the assumption that streamflow extremes (3 d maxima) at a set of gauge locations are realizations of a Gaussian elliptical copula and generalized extreme value (GEV) margins with nonstationary parameters. These parameters are modeled as a linear function of suitable covariates describing the previous season selected using the deviance information criterion (DIC). Finally, the copula is used to generate streamflow ensembles, which capture spatiotemporal variability and uncertainty. We apply this modeling framework to predict 3 d maximum streamflow in spring (May–June) at seven gauges in the Upper Colorado River basin (UCRB) with 0- to 2-month lead time. In this basin, almost all extremes that cause severe flooding occur in spring as a result of snowmelt and precipitation. Therefore, we use regional mean snow water equivalent and temperature from the preceding winter season as well as indices of large-scale climate teleconnections – El Niño–Southern Oscillation, Atlantic Multidecadal Oscillation, and Pacific Decadal Oscillation – as potential covariates for 3 d spring maximum streamflow. Our model evaluation, which is based on the comparison of different model versions and the energy skill score, indicates that the model can capture the space–time variability in extreme streamflow well and that model skill increases with decreasing lead time. We also find that the use of climate variables slightly enhances skill relative to using only snow information. Median projections and their uncertainties are consistent with observations, thanks to the representation of spatial dependencies through covariates in the margins and a Gaussian copula. This spatiotemporal modeling framework helps in the planning of seasonal adaptation and preparedness measures as predictions of extreme spring streamflows become available 2 months before actual flood occurrence.

OCRbayes: A Bayesian hierarchical modeling framework for Seahorse extracellular flux oxygen consumption rate data analysis

Background Mitochondrial dysfunction is involved in many complex diseases. Efficient and accurate evaluation of mitochondrial functionality is crucial for understanding pathology as well as facilitating novel therapeutic developments. As a popular platform, Seahorse extracellular flux (XF) analyzer is widely used for measuring mitochondrial oxygen consumption rate (OCR) in living cells. A hidden feature of Seahorse XF OCR data is that it has a complex data structure, caused by nesting and crossing between measurement cycles, wells and plates. Surprisingly, statistical analysis of Seahorse XF data has not received sufficient attention, and current methods completely ignore the complex data structure, impairing the robustness of statistical inference. Results To rigorously incorporate the complex structure into data analysis, here we developed a Bayesian hierarchical modeling framework, OCRbayes, and demonstrated its applicability based on analysis of published data sets. Conclusions We showed that OCRbayes can analyze Seahorse XF OCR experimental data derived from either single or multiple plates. Moreover, OCRbayes has potential to be used for diagnosing patients with mitochondrial diseases.