Cytopathic Effect of Vero Cells Adapted Bangladeshi Strain of Peste Des Petits Ruminants (PPR) Virus in Cell Culture

The present study described the cytopathic effect of PPR virus presently being used in serial passages at level of 60th in Vero cells and infected tissue culture fluid was used in this study as viral inoculum. Vero cells were grown on cover slip & were infected with tissue culture fluid at a fixed multiplicity of infection (MOI) 0.01. The infected cover slip along with control were stained with H&E stain at periodic intervals and cytopathic effect was studied with microscope. The cytopathic effect (CPE) was visible at first from 24 hpi and the Vero cells showed initial cell rounding, aggregation and syncytial development. Development of inclusion bodies and cell degradation was noticed by 72 hpi. Complete detachment of the cell monolayer was observed by 84 hpi. It is concluded that, development of numerous inclusion bodies is the indication of well adaptation & extensive multiplication of PPRV in Vero cells.

A new approach assessing the quality of whole slide images by discovering specific issues

Background : Since whole slide images (WSIs) are widely used in clinical diagnosis, assessing the quality of these images has generated considerable interest. We divided the evaluation process into different sections focusing on specific issues and developed an approach based on computer technology to assess the quality of WSIs and provide details corresponding to specific issues.Methods : We focused on three issues in this work: position deviation, cover slip misplacement and wrong focus. Our approach revolves around these issues. We collected two datasets from Guangdong Provincial People’s Hospital to develop the approach, discovered three issues and provided details corresponding to these issues.Results : We designed experiments based on two datasets to validate the effectiveness of the approach. The experiments show that using our approach, existing issues can be discovered and corresponding details are provided at a high accuracy. In the training set, the accuracy of the discovered three issues reaches 0.998, 0.987 and 0.988. In the validation set, the accuracy of discovering all issues reaches 0.914.Conclusions : We proposed an approach focusing on the following specific image issues: position deviation, cover slip misplacement and wrong focus. Using this approach, issues were discovered with high accuracy, and reports were produced that provide details regarding existing issues discovered by our approach. However, a dataset of WSIs with more issues, including stain concentration and poor microtomy, should be established, and methods discovering these issues must be developed in the future.

ID: 1060 Organic-inorganic Biomaterials promote mesenchymal stem cell proliferation and differentiation for long-term cultivation

Background: A nifty propagating of mesenchymal stem cell (MSCs) diligence has germinated all over the world by innovative investigators. However, the clinical and basic research applications of MSC requires novel finding biomaterials interfacial interaction especially in sustainable the morphology, physiology, multipotent and phenotypically in long-term cultivation. A prominent of biomaterials benefit to MSCs culture has triggered the multitudinous field especially in regenerative medicine. In order to hinder the deprivation of MSCs in purity and potency, the alternative cell-substrate materials of MSCs culture is essentially to be discovered. This has instigated the idea to encountered the method of screening libraries organic and inorganics biomaterials in bio-adhesively, free ethically, and sustainability to support the morphologically, physiologically, multi-potent and phenotypically of substrates coating cover slip. Methods: Libraries of inorganic biomaterials substrates have been collected from co-researcher to conduct the initial screening phase of 100 myriad fabrications of substrates whereas enumerated as a Graphene Oxide (GO), Hydroxyapatite (HAp), and Bioactive Glass (BAG) coated cover slip and discs also several organic biomaterials. Wharton’s Jelly derived Mesenchymal Stem Cells (WJMSCs) and Denuded Amnion Mesenchymal Stem Cells (AMMSCs) have been seeded on each substrate in the 48-well plate. Top four leading substrates have been selected for further cultivation until up to 5 passage (>P5) for long term screening known as scaling up phase. Several parameters such as cell attachment, cell viability, kinetic growth, cell-materials osteogenic and adipogenic differentiation and cell phenotype have been analyzed. Top one cell-material culture will go forward to further long-term cultivation up to Passage 10(>P10). Results: Morphologically and phenotypically demonstrated that GOy1WJMSC showed the significance result among others.

Ultra-low Anti-reflection Coating on a Plastic Cover Slip in Liquid for He-Ne Laser Light

An ultra-low anti-reflection optical coating on both surfaces of a plastic cover slip was studied for use in confocal image measurement. The optical reflectance at a wavelength of 632.8 nm was less than 0.1% when the coated sample was placed in a liquid having a refractive index of 1.34 close to the aqueous solution of the biomaterial. The high- and low-index coating films, Substance-2 (PrTiO3) and silicon dioxide (SiO2), were measured by an ellipsometer to determine their optical refraction indices and extinction coefficients. Theoretically, when the two layer thicknesses are designed using the optical admittance diagram of the cover slip to approach the equivalent index of 1.34, a reflectance of 1.6×10-5% in the liquid could be obtained. Experimentally, the reflectance of the sample deposited on the two faces of the cover slip was 4.223±0.145% as measured in the air; and 0.050±0.002% as measured by a He-Ne laser in the liquid.