scholarly journals Cytopathic Effect of Vero Cells Adapted Bangladeshi Strain of Peste Des Petits Ruminants (PPR) Virus in Cell Culture

2021 ◽  
Author(s):  
MSI Siddiqui ◽  
Anja Globig ◽  
Bernd Hoffmann ◽  
MN Islam ◽  
Islam ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Inclusion Bodies ◽  
Cytopathic Effect ◽  
Cell Monolayer ◽  
Culture Fluid ◽  
Vero Cells ◽  
Cover Slip ◽  
Ppr Virus ◽  
Complete Detachment ◽  
Bangladeshi Strain

Abstract The present study described the cytopathic effect of PPR virus presently being used in serial passages at level of 60th in Vero cells and infected tissue culture fluid was used in this study as viral inoculum. Vero cells were grown on cover slip & were infected with tissue culture fluid at a fixed multiplicity of infection (MOI) 0.01. The infected cover slip along with control were stained with H&E stain at periodic intervals and cytopathic effect was studied with microscope. The cytopathic effect (CPE) was visible at first from 24 hpi and the Vero cells showed initial cell rounding, aggregation and syncytial development. Development of inclusion bodies and cell degradation was noticed by 72 hpi. Complete detachment of the cell monolayer was observed by 84 hpi. It is concluded that, development of numerous inclusion bodies is the indication of well adaptation & extensive multiplication of PPRV in Vero cells.

Bilayer stabilizing peptides and the inhibition of viral infection: antimeasles activity of carbobenzoxy-Ser-Leu-amide

1987 ◽  
Vol 7 (9) ◽  
pp. 745-749 ◽  
Author(s):  
Richard M. Epand ◽  
Thomas J. Lobl ◽  
H. E. Renis
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Phase Transition Temperature ◽  
Measles Virus ◽  
Cytopathic Effect ◽  
Hexagonal Phase ◽  
Vero Cells ◽  
Time Parameter ◽  
Scanning Calorimetry ◽  
Hplc Retention Time

A number of carbobenzoxy-dipeptide-amides raise the bilayer to hexagonal phase transition temperature of dielaidoylphosphatidylethanolamine (stabilizes the bilayer). The potency of the peptides in stabilizing the bilayer phase is Z-Tyr-Leu-NH2>Z-Gly-Phe-NH2>Z-Ser-Leu-NH2>Z-Gly-Leu-NH2>Z-Gly-Gly-NH2. A linear correlation was found between the respective HPLC retention time parameter k′ for the peptide and the slope of the bilayer stabilization curve determined with model membranes by differential scanning calorimetry. One dipeptide, Z-Ser-Leu-NH2, reduces measles virus cytopathic effect (CPE) in Vero cells. The mechanism by which this peptide reduces the CPE is not known, although some peptides which raise the bilayer to hexagonal phase transition temperature of phospholipids inhibit membrane fusion.

shRNA targeting nonstructural protein inhibits the replication of severe fever with thrombocytopenia syndrome virus

2021 ◽  
Author(s):  
Lili Dou ◽  
Xiaoli Tao ◽  
Wei Zhao ◽  
Guofeng Zheng ◽  
Ying Lu ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Western Blotting ◽  
Virus Replication ◽  
Nonstructural Protein ◽  
Vero Cells ◽  
Antiviral Effects ◽  
Infective Dose ◽  
Shrna Plasmid ◽  
Dose Dependent ◽  
Severe Fever

Aim: To explore whether shRNA targeting nonstructural protein (NSs) of severe fever with thrombocytopenia syndrome virus (SFTSV) could inhibit SFTSV replication in Vero cells. Materials & methods: SFTSV used in this experiment was propagated in Vero cells and stored at -20°C. shRNA plasmid against NSs of SFTSV was transfected to Vero cells and infected with SFTSV, after which western blotting and tissue culture infective dose (TCID50) were used to measure the virus titers. Results: shRNA against NSs protein decreased the expression of NSs and inhibited the replication of SFTSV. Conclusion: The constructed SFTSV NSs-shRNA plasmid could inhibit the replication of SFTSV. It was concluded that SFTSV NSs-shRNA could inhibit virus replication for at least 72 h. shRNA-mediated antiviral effects were dose-dependent.

scholarly journals THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS

1967 ◽  
Vol 32 (1) ◽  
pp. 89-112 ◽  
Author(s):  
George G. Rose
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Mechanical System ◽  
Environmental Conditions ◽  
The Other ◽  
Cover Slip

A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microenvironment between the closely apposed cellophane and cover slip, whereas the other cell products move from chamber to chamber in the circulating nutrient. The resultant environmental conditions directed by the circumfusion systems are highly favorable for maintaining the differentiation of chick embryo tissues over protracted periods; a number of micrographs are shown.

scholarly journals Dengue Virus Type 1 Nonstructural Glycoprotein NS1 Is Secreted from Mammalian Cells as a Soluble Hexamer in a Glycosylation-Dependent Fashion

1999 ◽  
Vol 73 (7) ◽  
pp. 6104-6110 ◽  
Author(s):  
Marie Flamand ◽  
Françoise Megret ◽  
Magali Mathieu ◽  
Jean Lepault ◽  
Félix A. Rey ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Culture Fluid ◽  
Vero Cells ◽  
Soluble Form ◽  
Complex Type ◽  
Infected Insect ◽  
Infected Cells

ABSTRACT Nonstructural glycoprotein NS1, specified by dengue virus type 1 (Den-1), is secreted from infected green monkey kidney (Vero) cells in a major soluble form characterized by biochemical and biophysical means as a unique hexameric species. This noncovalently bound oligomer is formed by three dimeric subunits and has a molecular mass of 310 kDa and a Stokes radius of 64.4 Å. During protein export, one of the two oligosaccharides of NS1 is processed into an endo-β-N-acetylglucosaminidase F-resistant complex-type sugar while the other remains of the polymannose type, protected in the dimeric subunit from the action of maturation enzymes. Complete processing of the complex-type sugar appears to be required for efficient release of soluble NS1 into the culture fluid of infected cells, as suggested by the repressive effects of the N-glycan processing inhibitors swainsonine and deoxymannojyrimicin. These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell.

scholarly journals BIOCHEMICAL STUDY OF CELLULAR ANTIGEN-ANTIBODY REACTION IN TISSUE CULTURE

1960 ◽  
Vol 112 (2) ◽  
pp. 249-255 ◽  
Author(s):  
Akira Tokuda ◽  
Hideo Hayashi ◽  
Kinishiro Matsuba
Keyword(s):  
Tissue Culture ◽  
Protease Activity ◽  
Biochemical Study ◽  
Culture Fluid ◽  
Partial Purification ◽  
Trichloracetic Acid ◽  
Heat Stable ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Cellular Antigen

Decrease in the protease activity of the culture fluid observed at later stages of the antigen-antibody reaction is believed to be due to the release of an inhibitor by the cells. The inhibitor was submitted to partial purification: it is heat-stable, non-precipitated by trichloracetic acid and non-dialyzable. It inhibits certain cellular and tissue proteases and papain but is inactive against trypsin. It is suggested that the balance between protease and anti-protease released may determine the intensity, extent, and duration of certain sensitization phenomena.

scholarly journals Immunosuppressive activity of a subline of the mouse EL-4 lymphoma. Evidence for minute virus of mice causing the inhibition.

1976 ◽  
Vol 143 (1) ◽  
pp. 187-205 ◽  
Author(s):  
G D Bonnard ◽  
E K Manders ◽  
D A Campbell ◽  
R B Herberman ◽  
M J Collins
Keyword(s):  
Tissue Culture ◽  
Culture Fluid ◽  
Inhibitory Factor ◽  
Minute Virus Of Mice ◽  
G Cells ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Lymphocyte Cultures ◽  
Minute Virus ◽  
Kilham Rat Virus

Filtered culture fluids from the early in vitro passages of a subline of the C57BL/6 mouse EL-4 lymphoma, EL-4(G-), were strongly inhibitory for BABL/c vs. C57BL/6 mixed lymphocyte cultures (MLC). The inhibitory activity could be preserved by storage at -75 degrees C or 4 degrees C, thus allowing its further characterization. The inhibitory factor was particulate (nondialyzable, sedimentable at 100,000 g for 1 h), very small (recovered after 0.10 mum filtration), sensitive to UV irradiation, but heat stable (56 degrees C, 1 h) and resistant to chloroform. It was infectious, since later, noninhibitory passages of EL-4(G-) tissue culture cells became strongly inhibitory upon inoculation with the culture fluid. This data was consistent with the inhibitory factor being an infectious virus. Virus analysis by mouse antibody production tests revealed that viruses were indeed present in EL-4(G-) ascites cells and in the culture fluid, and not in a late passage of EL-4(G-) tissue culture cells which were not inhibitory. Neutralization of the inhibitory factor was achieved by pretreatment with ascitic fluid or with the sera raised against those (EL-4(G-)-derived materials which contained viruses. Mouse reference immune sera against minute virus of mice (MVM) completely neutralized the inhibitory factor in the culture fluid or in EL-4(G-) ascites cells. Two prototype MVM strains, and one Kilham rat virus preparation, did not inhibit the mouse MLC. Thus, the possibility exists that a variant of MVM, or an unidentified virus, has been grown and selected for in EL-4(G-) cells and recognized, due to its immunosuppressive characteristics. In any event, immunosuppression by EL-4(G-) cells was not mediated by the tumor cells, their metabolic products, or associated endogenous type C viruses, but by an exogenous virus, most likely a variant MVM with immunosuppressive characteristics. This adds weight to a parallel observation from our laboratory on the immunosuppressive effects of Kilham rat virus in rat lymphocyte cultures.

scholarly journals Effect of interferon on the development of Trypanosoma cruzi in tissue culture "Vero" cells

1980 ◽  
Vol 75 (1-2) ◽  
pp. 157-160 ◽  
Author(s):  
R. R. Golgher ◽  
M. S. M. Bertelli ◽  
M. L. Petrillo-Peixoto ◽  
Z. Brener
Keyword(s):  
Tissue Culture ◽  
Trypanosoma Cruzi ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vero Cells ◽  
Cell Infection ◽  
Amniotic Cells

Results are presented on the effects of interferon on the intracellular stages of T. cruzi in tissue culture "Vero" cells. Interferon was obtained by infecting monolayers of human amniotic cells with inactivated Newcastle disease virus. Interferon has not affected the cell infection by T. cruzi culture infective stages and neither has it prevented the transformation of amastigote into trypomastigote stages.

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