Broad phylogenetic and functional diversity among mixotrophic consumers of Prochlorococcus

Small eukaryotic phytoplankton are major contributors to global primary production and marine biogeochemical cycles. Many taxa are thought to be mixotrophic, but quantitative studies of phagotrophy exist for very few. In addition, little is known about consumers of Prochlorococcus, the abundant cyanobacterium at the base of oligotrophic ocean food webs. Here we describe thirty–nine new phytoplankton isolates from the North Pacific Subtropical Gyre (Station ALOHA), all flagellates ~2–5 μm diameter, and we quantify their ability to graze Prochlorococcus. The mixotrophs are from diverse classes (dictyochophytes, haptophytes, chrysophytes, bolidophytes, a dinoflagellate, and a chlorarachniophyte), many from previously uncultured clades. Grazing ability varied substantially, with specific clearance rate (volume cleared per body volume) varying over ten–fold across isolates and six–fold across genera. Slower grazers tend to create more biovolume per prey biovolume consumed. Using qPCR we found that the haptophyte Chrysochromulina was most abundant among the isolated mixotrophs at Station ALOHA, with 76–250 cells mL-1 across depths in the upper euphotic zone. Our results show that within a single ecosystem the phototrophs that ingest bacteria come from many branches of the eukaryotic tree, and are functionally diverse, indicating a broad range of strategies along the spectrum from phototrophy to phagotrophy.

Climate-driven phytoplankton community shifts in the North Pacific Subtropical Gyre

<p><span>Natural climate variability influences phytoplankton community both directly and indirectly by altering ocean stratification and availabilities of nutrient and light, or grazing pressure. The world’s largest ecosystem, North Pacific Subtropical Gyre (NPSG), is largely controlled by basin-scale decadal climate variability, such as the North Pacific Gyre Oscillation and the Pacific Decadal Oscillation. These indices have two phases, known as warm phase and cool phase, respectively. Previous studies reported that warm phase was related to the dominance of pico-phytoplankton induced by warm temperature anomaly (i.e., strong stratification) while cool phase was related to the dominance of nano-phytoplankton induced by cold temperature anomaly (i.e., weak stratification). Besides the impact of natural climate variability, anthropogenic global warming has accelerated in recent years and it might have abnormal impact on marine ecosystems. However, there is little information about the responses of phytoplankton community to recent climate change in the NPSG. Here, we present the temporal variations of deseasonalized and normalized NPSG phytoplankton community using phytoplankton pigment concentrations and cell densities, obtained on monthly intervals over the period 1988−2018 at Station ALOHA (22°45’N, 158°W). These variations were compared with the variations of climate indices, physical, and biogeochemical parameters from Station ALOHA. The NPSG climate indices showed five phase transitions; warm (~1997) – cool (1998−2002) – warm (2003−2006) – cool (2007−2013) – warm (2014~). Before 2006 year, the phase transitions of phytoplankton community (pico→n</span><span>ano→</span><span>pico) were coincident with physical factors (e.g., stratification; strong→</span><span>weak→</span><span>strong) and biogeochemical factors (e.g., particle export; low→</span><span>high→</span><span>low), coupling with phases of climate indices. However, interestingly, following the recent rapid rise in greenhouse gas emission (since 2007), phytoplankton community, even under continued coupling of climate indices and physical factors, showed only dominance of pico-phytoplankton, decoupling with the phases of climate indices. These findings suggest that the contribution of pico-sized plankton to NPSG phytoplankton community will increase gradually in response to the acceleration of the global warming.</span></p>