Hybride necrosis genes in bread wheat varieties (Triticum aestivum L.) of Ukraine

Hybrid necrosis is the gradual premature death of leaves or plants in certain F1-2hybrids of wheat (Triticum aestivum L.), and it is caused by the interaction of two dominant complementary genes Ne1 and Ne2 located on chromosome arms 5BL and 2BS, respectively. To date, necrotic genotypes in most varieties of Ukrainian breeding have not been identified. Aim. This study was conducted to determine the necrotic genotypes in varieties Ukrainian breeding of different regions. Methods. Microsatellite analysis, PAAgel-electrophoresis. Results. 150 genotypes of bread wheat varieties from Ukrainian breeding were identified by loci Xbarc74-5В and Xbarc55-2В closely linked to hybrid necrosis genes Ne1 and Ne2, respectively. Conclusions. The most common in the South of Ukraine is the genotypes Ne1wNe2w/m and Ne1mNe2w/m were revealed. The most of varieties in the North of Ukraine had the genotypes ne1Ne2ms. These genotypes can have breeding and adaptive value for specific geographical conditions. Keywords: Triticum aestivum L., hybride necrosis genes Ne1 and Ne2, microsatellite analysis.