Complete Genome Sequence of the Halophilic Methylotrophic Methanogen Archaeon Methanohalophilus portucalensis Strain FDF-1 T

ABSTRACT We report here the complete genome sequence (2.08 Mb) of Methanohalophilus portucalensis strain FDF-1 T , a halophilic methylotrophic methanogen isolated from the sediment of a saltern in Figeria da Foz, Portugal. The average nucleotide identity and DNA-DNA hybridization analyses show that Methanohalophilus mahii , M. halophilus , and M. portucalensis are three different species within the Methanosarcinaceae family.

Draft Genome Sequence ofMethanohalophilus mahiiStrain DAL1 Reconstructed from a Hydraulic Fracturing-Produced Water Metagenome

We report here the 1,882,100-bp draft genome sequence ofMethanohalophilus mahiistrain DAL1, recovered from Marcellus Shale hydraulic fracturing-produced water using metagenomic contig binning. Genome annotation revealed several key methanogenesis genes and provides valuable information on archaeal activity associated with hydraulic fracturing-produced water environments.

The Genome Sequence ofMethanohalophilus mahiiSLPTReveals Differences in the Energy Metabolism among Members of theMethanosarcinaceaeInhabiting Freshwater and Saline Environments

Methanohalophilus mahiiis the type species of the genusMethanohalophilus, which currently comprises three distinct species with validly published names.Mhp. mahiirepresents moderately halophilic methanogenic archaea with a strictly methylotrophic metabolism. The type strain SLPTwas isolated from hypersaline sediments collected from the southern arm of Great Salt Lake, Utah. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,012,424 bp genome is a single replicon with 2032 protein-coding and 63 RNA genes and part of theGenomic Encyclopedia of Bacteria and Archaeaproject. A comparison of the reconstructed energy metabolism in the halophilic speciesMhp. mahiiwith other representatives of theMethanosarcinaceaereveals some interesting differences to freshwater species.