Debottlenecking Thermophilic Cyanobacteria Cultivation and Harvesting through the Application of Inner-Light Photobioreactor and Chitosan

Thermophilic cyanobacteria are a low-carbon environmental resource with high potential thanks to their innate temperature tolerance and thermostable pigment, phycocyanin, which enhances light utilisation efficiency and generates a high-value product. However, large-scale cultivation and harvesting have always been bottlenecks in unicellular cyanobacteria cultivation due to their micrometric size. In this study, a 40-litre inner-light photobioreactor (PBR) was designed for scaled-up cultivation of Thermosynechococcus elongatus E542. By analysing light transmission and attenuation in the PBR and describing it via mathematical models, the supply of light energy to the reactor was optimised. It was found that the hyperbolic model describes the light attenuation characteristics of the cyanobacterial culture more accurately than the Lambert–Beer model. The internal illumination mode was applied for strain cultivation and showed a two-fold better growth rate and four-fold higher biomass concentration than the same strain grown in an externally illuminated photobioreactor. Finally, the downstream harvesting process was explored. A mixture of chitosan solutions was used as a flocculant to facilitate biomass collection. The effect of the following parameters on biomass harvesting was analysed: solution concentration, flocculation time and flocculant concentration. The analysis revealed that a 4 mg L−1 chitosan solution is optimal for harvesting the strain. The proposed solutions can improve large-scale cyanobacterial biomass cultivation and processing.

BioZero—Designing Nature-Inspired Net-Zero Building

This article introduces BioZero, a nature-inspired near-zero building proposed for Quay St, Brooklyn, New York. The building is designed for the maximum use of daylight and natural ventilation. This is the result of its shallow plan depth and the inner light wells/ventilation stacks, which also serve the inner circulation space. The light wells/ventilation stacks are created as a result of the organic shape of the internal partitions. The building is constructed from a steel frame and hemp-lime bio-composite material (hempcrete), which smooths out the fluctuations of internal air temperature and relative humidity. The south facing façade is fitted with the Cadmium Telluride (CdTe) photovoltaic array that covers 90% of the opaque surface area of the façade. The design was based on nature-inspired computation, with sustainability principles as guiding constraints. The main findings are that the building achieves −227 tonnes of negative embodied carbon due to sequestration of CO2 in the hemp plant from which the material was harvested, and a net-zero operation. The main conclusions are that in the context of climate emergency, nature inspired design leads to energy efficient buildings with a high level of thermal comfort, which are buildable and sustainable.

6. Early Musical Education as a Premise for the Development of the Child’s Upright Personality

A person upright development is essentially connected to communication, and music is the language with the highest degree of communication, is the purest and deepest language, able to express the hidden, the essential, the spiritual. Musical education, achieved in all forms, contexts, periods, etc. under the mark of the upright development of human personality, means “wisdom, balance, love, inner light, harmony, “feeling”, etc.”. [I. Gagim]. Childhood, for this purpose, remains the fundamental stage for the subsequent development and initiation of the child in the “real” world, constituting at the same time, the foundation for his/her human “ becoming”.