The complete mRNA sequence of one tobacco (nicotiana tabacum) gene—2-hydroxyisoflavanone dehydratasedase, was amplified using the rapid amplification of cDNA ends methods based on one tobacco EST. The full-length tobacco 2-hydroxyisoflavanone dehydratasedase gene mRNA was 1,278bp containing a 966 bp open reading frame, which encodes a protein of 321 amino acids. Sequence analysis revealed that the 2-hydroxyisoflavanone dehydratasedase of tobacco shares high homology with the 2-hydroxyisoflavanone dehydratasedase of nicotiana tomentosiformis(99%), capsicum annuum(78%), potato(75%), lycopersicon pennellii(73%) and lycopersicon esculentum(72%). BLAST analysis within the tobacco high throughout genomic sequences database revealed that this gene has no intron and is a single exon gene. Results also showed that tobacco 2-hydroxyisoflavanone dehydratasedase gene has a closer genetic relationship with the 2-hydroxyisoflavanone dehydratasedase gene of nicotiana tomentosiformis. Tissue expression profile analysis revealed that the tobacco 2-hydroxyisoflavanone dehydratasedase gene was highly expressed in leaf and flower, but moderately expressed in root and stem. Our experiment established the foundation for further research on this tobacco gene.
Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) 2-Hydroxyisoflavanone Dehydratasedase Gene
