Giant Cell Tumor of Lower End of Tibia

Introduction. Giant cell tumor of bones is an unusual neoplasm that accounts for 4% of all primary tumors of bone, and it represents about 10% of malignant primary bone tumors with its different grades from borderline to high grade malignancy.Case Report. A 35-year-old patient presented with complains of pain and swelling in left ankle since 1 year following a twisting injury to his left ankle. On examination, swelling was present over the distal and anterior part of leg and movements of ankle joint were normal. All routine blood investigations were normal. X-ray and CT ankle showed morphology of subarticular well-defined expansile lytic lesion in lower end of left tibia suggestive of giant cell tumor. Histopathology of the tissue shows multinucleated giant cells with uniform vesicular nucleus and mononuclear cells which are spindle shaped with uniform vesicular nucleus suggestive of GCT. The patient was treated by excision, curettage, and bone cement to fill the defect.Conclusion. The patient at 12-month followup is doing well and walking without any pain comfortably and with full range of motion at ankle joint with articular congruity maintained and no signs of recurrences.

Homologs of Leydig and Sertoli Cells in the Testis of the Teleost, Oryzias Latipes

The testis of O. latipes is composed of cysts containing germinal elements Tn various stages of spermatogenesis (Fig. 1). The simple epithelial cells of the cyst wall are the Sertoli cell (SC) homologs. When the contained germinal elements have matured, the cyst epithelium fuses with that of the terminal divisions of the efferent duct system. In the connective tissue stroma surrounding the cysts and ducts, the Leydig cell (LC) homologs are found.The LC (Fig. 2) contains a central vesicular nucleus with a distinct nucleolus. Flattened cisternae of rough ER are distributed throughout the cytoplasm; smooth ER, usual in steroidogenic cells, is not present.

The Ultrastructure of Phytohemagglutinin (PHA) Stimulated Lymphocytes of Chronic Lymphatic Leukemia

Cultured lymphocytes of chronic lymphatic leukemia have been shown to have a delayed and diminished response to phytohemagglutinin (PHA) stimulation. By electron microscopic examination, a morphologically distinct intermediate blastoid cell was identified in the stimulated cultures which showed minimal differences between those derived from normal and leukemic lymphocytes. It was distinguished by a large vesicular nucleus and relatively scant cytoplasm with few organelles. This intermediate blastoid cell frequently predominated in cultures with a low total percentage of blastogenesis. It is suggested that this intermediate blastoid cell represents a premitotic phase in lymphocytic transformation, though the possibility of it’s being a nonmitotic blastoid cell cannot be excluded. No morphologic differences were noted between the large blastoid cells transformed from normal and leukemic lymphocytes.

A new flagellate of the genus Proteromonas Kunstler, 1883, from an Indian lizard

A new flagellate, Proteromonas hemidactyli sp.nov., is described from the large intestine of the lizard, Hemidactylus brooki, in Hyderabad, India. It is characterized by a long, slender and fusiform body, two similar but subequal flagella, a kidney-shaped sub-blepharoplastic body, a ribbon-like paranuclear body and a vesicular nucleus. A parabasal body is absent.I am extremely grateful to Dr S. S. Qadri, Reader in Zoology, Osmania University, Hyderabad for his guidance and advice during the course of this work. I am thankful to Dr S. Mehdi Ali, Professor of Zoology, Marathwada University, and Dr S. N. Singh, Professor of Zoology, Osmania University, for providing me with laboratory and library facilities. Thanks are also due to the Council of Scientific and Industrial Research, New Delhi, for the award of a fellowship.

The Life History of Amoebae of the Limax Type in the Human Intestine

At the time investigators were trying to cultivate the amoebae of the human intestine (Entamoeba coli and E. histolytica), amoebae were often encountered in the cultures which were referred to the species “Amoeba limax,” with many sub-species. Subsequently it was found that the cysts of these cultural amoebae are very common and that it is possible to cultivate amoebae from nearly every source. Consequently it was concluded that these forms do not really live in the human intestine but that the cultural amoebae developed from cysts, occasionally ingested with food, the cysts not having developed in the intestine. This is Walker's (1911) view, but Chatton and Lalung Bonaire (1912) hold that the limax amoebae (hereafter called limax) can live in the intestine, not only in the form of cysts but also as motile amoebae. Cultures made from these stools showed amoebae and uninucleate cysts. The latter were not found in the faeces, which showed only the motile stages, without however any signs of division. The cultures showed this amoeba to be of the common Umax-type with a vesicular nucleus containing a large karyosome. These observations are important because they contradict the hypothesis that Entamoebae when cultivated show the features of limax.