Automatic Vehicle Access System using Ultra-High Frequency RFID

Securing and monitoring the territory have become a big challenge for everyone as the number of vehicle users have increased. The premise area must be secure with enforced entrances and exits. It should have an automated and efficient security and monitoring system. For this purpose, an automated vehicle access system with accurate and secure monitoring of vehicle entry and exit was developed with Graphical User Interface (GUI) and vehicle’s user database along with the prototype of Ultra-High Frequency RFID. The suitable configuration for the hardware prototype has also been evaluated to maximize the system’s capabilities. This system operates on frequencies from 400MHz to 950MHz and is performed at distances of 7 meters. The passive tag was attached to the various condition and sizes of the car. The result shows showed that the system was optimally performed with the speed of the vehicle up to 50 km/h, 7 m of reading range and a maximum 20% tinted windshield. The ideal angle for the reader is around 60ᵒ to 70ᵒ with 2 meters of mounting pole. For future improvement, it is suggested that the registered vehicle user data was linked with the official premise’s database and the data is saved in an off-site location (cloud storage). The GUI is also able to support more than 1 reader at a time depending on demand.

Performance Evaluation of the KRYPTOR Compact PLUS Analyzer-Based B.R.A.H.M.S. CgA Ⅱ KRYPTOR Assay for Chromogranin A Measurement

Numerous immunoassays have been developed to measure the levels of chromogranin A (CgA), a useful biomarker for diagnosing and monitoring generally heterogeneous neuroendocrine tumors (NETs). Here, we evaluated the imprecision and linearity of three such assays: KRYPTOR (ThermoFisher Scientific), NEOLISA (EuroDiagnostica), and CgA-RIA (CisBio), using 123 samples for each assay. The correlation coefficients between the assays were 0.932 (CgA-RIA versus NEOLISA), 0.956 (KRYPTOR versus CgA-RIA), and 0.873 (NEOLISA versus KRYPTOR). KRYPTOR showed good precision, with percent coefficients of variation less than 5% for low and high concentration quality controls. Linearity was maintained over a wide concentration range. Comparison of CgA levels from three disease entities (NETs, non-NET pancreatic tumors, and prostate cancer) and healthy controls showed that patients with NETs had significantly higher CgA levels (n = 57, mean: 1.82 ± 0.43 log ng/mL) than healthy individuals (n = 20, mean: 1.51 ± 0.23 log ng/mL; p = 0.018). No other significant differences between groups were observed. All three immunoassays showed strong correlations in measured CgA levels. Because KRYPTOR operation uses a fully automated random-access system and requires shorter incubation times and smaller sample volumes, the KRYPTOR assay may improve laboratory workflow while maintaining satisfactory analytical performance.