scholarly journals Performance Evaluation of the KRYPTOR Compact PLUS Analyzer-Based B.R.A.H.M.S. CgA Ⅱ KRYPTOR Assay for Chromogranin A Measurement

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2400
Author(s):  
Yu Jeong Choi ◽  
Juhye Roh ◽  
Sinyoung Kim ◽  
Kyung-A Lee ◽  
Younhee Park
Keyword(s):  
Chromogranin A ◽  
Random Access ◽  
Correlation Coefficients ◽  
Pancreatic Tumors ◽  
Good Precision ◽  
Strong Correlations ◽  
High Concentration ◽  
Access System ◽  
Coefficients Of Variation ◽  
Disease Entities

Numerous immunoassays have been developed to measure the levels of chromogranin A (CgA), a useful biomarker for diagnosing and monitoring generally heterogeneous neuroendocrine tumors (NETs). Here, we evaluated the imprecision and linearity of three such assays: KRYPTOR (ThermoFisher Scientific), NEOLISA (EuroDiagnostica), and CgA-RIA (CisBio), using 123 samples for each assay. The correlation coefficients between the assays were 0.932 (CgA-RIA versus NEOLISA), 0.956 (KRYPTOR versus CgA-RIA), and 0.873 (NEOLISA versus KRYPTOR). KRYPTOR showed good precision, with percent coefficients of variation less than 5% for low and high concentration quality controls. Linearity was maintained over a wide concentration range. Comparison of CgA levels from three disease entities (NETs, non-NET pancreatic tumors, and prostate cancer) and healthy controls showed that patients with NETs had significantly higher CgA levels (n = 57, mean: 1.82 ± 0.43 log ng/mL) than healthy individuals (n = 20, mean: 1.51 ± 0.23 log ng/mL; p = 0.018). No other significant differences between groups were observed. All three immunoassays showed strong correlations in measured CgA levels. Because KRYPTOR operation uses a fully automated random-access system and requires shorter incubation times and smaller sample volumes, the KRYPTOR assay may improve laboratory workflow while maintaining satisfactory analytical performance.

scholarly journals Performance evaluation of the KRYPTOR Compact PLUS analyzer-based B.R.A.M.S CgA II KRYPTOR assay for chromogranin A measurement

2021 ◽  
Author(s):  
Yu Jeong CHOI ◽  
Juhye Roh ◽  
Sinyoung Kim ◽  
Kyung-A Lee ◽  
Younhee Park
Keyword(s):  
Chromogranin A ◽  
Random Access ◽  
Correlation Coefficients ◽  
Pancreatic Tumors ◽  
Good Precision ◽  
Strong Correlations ◽  
High Concentration ◽  
Access System ◽  
Coefficients Of Variation ◽  
Disease Entities

Abstract Background: Numerous immunoassays have been developed to measure the levels of chromogranin A (CgA), a useful biomarker for diagnosing and monitoring generally heterogeneous neuroendocrine tumors (NETs). Here, we evaluated the imprecision and linearity of three such assays: KRYPTOR (ThermoFisher Scientific), NEOLISA (EuroDiagnostica), and CgA-RIA (CisBio) using 123 samples for each assay.Results: The correlation coefficients between the assays were 0.932 (CgA-RIA versus NEOLISA), 0.956 (KRYPTOR versus CgA-RIA), and 0.873 (NEOLISA versus KRYPTOR). KRYPTOR showed good precision, with percent coefficients of variation less than 5% for low and high concentration quality controls. Linearity was maintained over a wide concentration range. Comparison of CgA levels from three disease entities (NETs, non-NET pancreatic tumors, and prostate cancer) and healthy controls showed that patients with NETs had significantly higher CgA levels (n = 57, mean: 1.82 ± 0.43 log ng/mL) than healthy individuals (n = 20, mean: 1.51 ± 0.23 log ng/mL; P = 0.018). No other significant differences between groups were observed.Conclusion: All three immunoassays showed strong correlations in measured CgA levels. Because KRYPTOR operation uses a fully automated random-access system and requires shorter incubation times and smaller sample volumes, the KRYPTOR assay may improve laboratory workflow while maintaining satisfactory analytical performance.

Dual Analyte Immunoassay—a New Approach to Neural Tube Defect and Down's Syndrome Screening

1992 ◽  
Vol 29 (4) ◽  
pp. 390-396 ◽  
Author(s):  
J N Macri ◽  
K Spencer ◽  
R Anderson
Keyword(s):  
Neural Tube ◽  
Neural Tube Defect ◽  
Detection Efficiency ◽  
Correlation Coefficients ◽  
Microtiter Plate ◽  
Good Precision ◽  
Coefficients Of Variation ◽  
Quality Of Patient Care ◽  
Β Hcg

A microtiter plate based Dual Analyte enzymeimmunoassay method for the simultaneous measurement of α-fetoprotein (AFP) and Free-β human chorionic gonadotrophin (hCG) was evaluated. This rapid assay, which has application in both Neural Tube Defect screening and Down's screening, shows good precision with between assay coefficients of variation between 5 and 7·5% for AFP and 3·7 to 5·8% for Free-β(hCG). Correlation with single analyte procedures is good, with correlation coefficients being greater than 0·91 in both cases. Clinical discrimination in detecting both types of abnormalities is not compromised by this new simultaneous Dual Analyte assay. We conclude that the Dual Analyte approach, which combines analytes achieving the highest known detection efficiency, will bring about improvements in the efficiency of screening, reduce costs and improve report turnaround, all leading to better quality of patient care.

scholarly journals Portable Force Plates: A Viable and Practical Alternative to Rapidly and Accurately Monitor Elite Sprint Performance

Sports ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 61 ◽  
Author(s):  
Irineu Loturco ◽  
Lucas Pereira ◽  
Ronaldo Kobal ◽  
Cesar Cal Abad ◽  
Victor Fernandes ◽  
...  
Keyword(s):  
Vertical Jump ◽  
Force Plate ◽  
Correlation Coefficients ◽  
Accurate Information ◽  
Strong Correlations ◽  
Regression Analyses ◽  
Actual Performance ◽  
Moment Coefficient ◽  
Competitive Athletes ◽  
Vertical Jumps

This study aimed to examine the associations between a series of mechanical variables automatically generated by a portable force plate (PFP) and the actual performance of professional sprinters over a 150 m course. To test these correlations, 12 top-level sprinters performed vertical jumps (squat and countermovement jumps; SJ and CMJ, respectively), a 60 m sprint test, and a 150 m sprint test. Pearson product-moment coefficient of correlation and multiple regression analyses were used to determine the relationships between the sprinting velocities and vertical jump outputs. The SJ parameters were moderately to near perfectly associated with the different sprint distances, and the SJ height presented the highest correlation scores (r = 0.90 with velocities over 10- and 20-m). The correlation coefficients between the CMJ outcomes and sprint results varied between moderate and very large (from 0.38 to 0.88). Finally, the coefficients of determination (R2) ranged from 0.71 to 0.85 for the different multiple regressions involving PFP automatic measures. The PFP can provide practitioners with quick and accurate information regarding competitive athletes. Due to the strong correlations observed, coaches are encouraged to frequently adjust and tailor the training strategies of their sprinters, using practical and timesaving PFP measurements.

scholarly journals Relationships between Meteorological Parameters and Particulate Matter in Mae Hong Son Province, Thailand

2018 ◽  
Vol 15 (12) ◽  
pp. 2801 ◽  
Author(s):  
Wissanupong Kliengchuay ◽  
Aronrag Cooper Meeyai ◽  
Suwalee Worakhunpiset ◽  
Kraichat Tantrakarnapa
Keyword(s):  
Air Pollution ◽  
Particulate Matter ◽  
Meteorological Parameters ◽  
Warning System ◽  
Correlation Coefficients ◽  
Pm10 Concentration ◽  
P Value ◽  
Climate Data ◽  
High Concentration ◽  
Ambient Concentrations

Meteorological parameters play an important role in determining the prevalence of ambient particulate matter (PM) in the upper north of Thailand. Mae Hong Son is a province located in this region and which borders Myanmar. This study aimed to determine the relationships between meteorological parameters and ambient concentrations of particulate matter less than 10 µm in diameter (PM10) in Mae Hong Son. Parameters were measured at an air quality monitoring station, and consisted of PM10, carbon monoxide (CO), ozone (O3), and meteorological factors, including temperature, rainfall, pressure, wind speed, wind direction, and relative humidity (RH). Nine years (2009–2017) of pollution and climate data obtained from the Thai Pollution Control Department (PCD) were used for analysis. The results of this study indicate that PM10 is influenced by meteorological parameters; high concentration occurred during the dry season and northeastern monsoon seasons. Maximum concentrations were always observed in March. The PM10 concentrations were significantly related to CO and O3 concentrations and to RH, giving correlation coefficients of 0.73, 0.39, and −0.37, respectively (p-value < 0.001). Additionally, the hourly PM10 concentration fluctuated within each day. In general, it was found that the reporting of daily concentrations might be best suited to public announcements and presentations. Hourly concentrations are recommended for public declarations that might be useful for warning citizens and organizations about air pollution. Our findings could be used to improve the understanding of PM10 concentration patterns in Mae Hong Son and provide information to better air pollution measures and establish a warning system for the province.

scholarly journals Genetic variability of rye-xenic-hybrid traits

2014 ◽  
Vol 65 (3-4) ◽  
pp. 329-333 ◽  
Author(s):  
Lechosław Grochowski ◽  
Jan Kaczmarek ◽  
Władysław Kadłubiec ◽  
Henryk Bujak
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Plant Height ◽  
Combining Ability ◽  
Field Experiments ◽  
Correlation Coefficients ◽  
Winter Rye ◽  
Arithmetic Means ◽  
Coefficients Of Variation ◽  
Standard Cultivar

In field experiments performed in two localities (Smolice, Wrocław) 18 xenic hybrids of winter rye, two testers and standard cultivar Dańkowskie Złote, were analysed. The objects of detailed evaluations were 11 traits. For six of them arithmetic means (x), standard deviations (S), coefficients of variation (cv), coefficients of genetic diversity (h<sup>2</sup>), correlation coefficients were calculated. Moreover, analyses of variance were carried out and the effects of general (GCA) and specific (SCA) combining ability were estimated. The existence of quantitative xenia in hybrids was confirmed. It was shown that xenic hybrids, in respect to most of the analysed traits, were insignificantly inferior to the testers and the standard cultivar. However, the decrease of plant height has shown to be significant and a tendency to higher yield was observed.

scholarly journals Reproducibility of Fixed-luminance and Multi-luminance Flicker Electroretinography in Patients With Diabetic Retinopathy Using an Office-based Testing Paradigm

2019 ◽  
Vol 14 (6) ◽  
pp. 1095-1103 ◽  
Author(s):  
John J. Wroblewski ◽  
Christa McChancy ◽  
Kassandra Pickel ◽  
Hunter Buterbaugh ◽  
Tyler Wieland ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Correlation Coefficients ◽  
Significant Negative Correlation ◽  
Fundus Photography ◽  
University Of Wisconsin ◽  
Coefficients Of Variation ◽  
Department Of Ophthalmology ◽  
Patients With Diabetes ◽  
Pearson Correlations ◽  
Reading Center

Background: We evaluated the reproducibility of office-based flicker electroretinography (ERG) in patients with nonproliferative diabetic retinopathy (NPDR). Methods: An observational study was conducted in which ultra-widefield fluorescein angiography (UWF-FA) was performed on 20 patients with mild-to-moderate NPDR; images were graded by the Fundus Photography Reading Center (Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, WI, USA). Fixed- and multi-luminance flicker ERG was repeated four times (greater than or equal to seven days apart). Recording consistency was assessed using intra-class correlation coefficients (ICCs), coefficients of variation, and Pearson correlations. Results: 82.5% and 17.5% of eyes had mild and moderate NPDR using UWF-FA; 90% of the angiograms were given a high confidence grade. Fixed-luminance phase values were highly reproducible (ICC: 0.949; P < .001). There was a significant negative correlation between fixed-luminance phase and log-corrected ischemic index values (−0.426; P = .015). Conclusions: Office-based, fixed-luminance phase values are highly reproducible and negatively correlate with retinal ischemia in NPDR, suggesting that global retinal dysfunction may be reliably quantified early in patients with diabetes.

scholarly journals Action Boundary Proximity Effects on Perceptual-Motor Judgments

2019 ◽  
Vol 90 (12) ◽  
pp. 1000-1008
Author(s):  
Caleb D. Johnson ◽  
Alice D. LaGoy ◽  
Gert-Jan Pepping ◽  
Shawn R. Eagle ◽  
Anne Z. Beethe ◽  
...  
Keyword(s):  
Intraclass Correlation ◽  
Reaction Times ◽  
Correlation Coefficients ◽  
Proximity Effects ◽  
Testing Time ◽  
Good Reliability ◽  
Intraclass Correlation Coefficients ◽  
Coefficients Of Variation ◽  
Time Required ◽  
Action Coupling

INTRODUCTION: Designed as a more ecological measure of reaction times, the Perception-Action Coupling Task (PACT) has shown good reliability and within-subject stability. However, a lengthy testing period was required. Perceptual-motor judgments are known to be affected by proximity of the stimulus to the action boundary. The current study sought to determine the effects of action boundary proximity on PACT performance, and whether redundant levels of stimuli, eliciting similar responses, can be eliminated to shorten the PACT.METHODS: There were 9 men and 7 women who completed 4 testing sessions, consisting of 3 familiarization cycles and 6 testing cycles of the PACT. For the PACT, subjects made judgments on whether a series of balls presented on a tablet afford “posting” (can fit) through a series of apertures. There were 8 ratios of ball to aperture size (B-AR) presented, ranging from 0.2 to 1.8, with each ratio appearing 12 times (12 trials) per cycle. Reaction times and judgment accuracy were calculated, and averaged across all B-ARs. Ratios and individual trials within each B-AR were systematically eliminated. Variables were re-averaged, and intraclass correlation coefficients (ICC) and coefficients of variation (CVTE) were calculated in an iterative manner.RESULTS: With elimination of the 0.2 and 1.8 B-ARs, the PACT showed good reliability (ICC = 0.81–0.99) and consistent within-subject stability (CVTE = 2.2–14.7%). Reliability (ICC = 0.81–0.97) and stability (CVTE = 2.6–15.6%) were unaffected with elimination of up to 8 trials from each B-AR.DISCUSSION: The shortened PACT resulted in an almost 50% reduction in total familiarization/testing time required, significantly increasing usability.Johnson CD, LaGoy AD, Pepping G-J, Eagle SR, Beethe AZ, Bower JL, Alfano CA, Simpson RJ, Connaboy C. Action boundary proximity effects on perceptual-motor judgments. Aerosp Med Hum Perform. 2019; 90(12):1000–1008.

scholarly journals The Yo-Yo Intermittent Endurance Level 2 Test: Reliability of Performance Scores, Physiological Responses and Overload Characteristics in Competitive Soccer, Basketball and Volleyball Players

2019 ◽  
Vol 67 (1) ◽  
pp. 223-233 ◽  
Author(s):  
Konstantinos Papanikolaou ◽  
Athanasios Chatzinikolaou ◽  
Theodoros Pontidis ◽  
Alexandra Avloniti ◽  
Chariklia K. Deli ◽  
...  
Keyword(s):  
Correlation Coefficients ◽  
Total Sample ◽  
Test Reliability ◽  
Maximum Speed ◽  
Endurance Capacity ◽  
Intra Class Correlation ◽  
Coefficients Of Variation ◽  
Volleyball Players ◽  
Body Load ◽  
Level 2

AbstractThe purpose of this study was to evaluate the reliability and reproducibility of the physiological and overload features of the Yo-Yo intermittent endurance test level 2 (Yo-Yo IE2) in competitive male soccer (n = 20), basketball (n = 11), and volleyball players (n = 10). The participants completed Yo-Yo IE2 tests on three separate occasions with assessment of performance, heart rate, running speed, accelerations, decelerations and body load using GPS instrumentation. The intra-class correlation coefficient index, confidence intervals and coefficients of variation were calculated to assess the reliability of the test. Intra-class correlation coefficients for test-retest trials in the total sample ranged from large to nearly perfect (total distance: 0.896; mean speed: 0.535; maximum speed: 0.715; mean HR: 0.876; maximum HR: 0.866; body load: 0.865). The coefficients of variation for distance, mean speed, HR response, as well as acceleration and deceleration scores for test-retest trials ranged from 1.2 to 12.5% with no differences observed among particular sport disciplines. The CV for shuttles performed ranged from 4.4 to 5.5% in all sports. Similar results were obtained for the three different categories of players tested. These results suggest that the Yo-Yo IE2 test appears to be a reliable alternative for evaluating the ability to perform intermittent high-intensity running in different outdoor and indoor team sports. Players may need one or two familiarization tests to ensure valid assessment of intermittent endurance capacity. It appears that the Yo-Yo IE2 test incorporates accelerations and decelerations in a consistent and reproducible fashion.

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