Mast Cell Stabilizing, Antianaphylactic and Bronchodilatory activity of Methanolic extract of Averrhoa carambola Fruit

This work was mainly aimed to study the mast cell stabilizing, anti-anaphylactic and bronchodilatory activities of methanolic extract of Averrhoa carambola (ACME). Mast cell stabilization activity was investigated by Compound 48/80 induced mast cell degranulation in rats and antianaphylactic activity was performed by determining the mortality rate of mice upon exposure to compound 48/80. The bronchodilatory effect of ACME was studied on histamine aerosol-induced bronchospasm using guinea pigs, in which occurrence of preconvulsive dyspnea (PCD) was noted as end point. Treatment with ACME (100, 200 and 400mg/kg) showed significant (p<0.05) protection of rat peritoneal mast cells and significantly (p<0.05) reduced the mortality of mice in a dose dependent manner. ACME significantly (p<0.05) increased the time of preconvulsive dyspnea (PCD) in a dose dependent manner that suggestive of bronchodilating activity. Phytochemical studies observed presence of saponins, tannins, steroids, alkaloids, flavonoids and glycosides. From these finding, we concluded that ACME possesses mast cell stabilizing; anti anaphylactic and bronchodilatory activity which might be used in treatment of asthma.

Therapeutic Activity of Polyherbal Formulation against Passive Anaphylaxis by Using Rat Mesenteric Mast Cells

Anaphylaxis is the result of alteration in mast cell physiology and is responsible for the various physiological changes which are observed during anaphylaxis. The symptoms of anaphylaxis are due to the release of the histamine from the mast cells and it results mainly due to the degranulation of the mast cells. Antianaphylactic activity of polyherbal formulation against passive anaphylaxis was evaluated with the help of sheep serum induced passive anaphylaxis model and passive paw anaphylaxis model. Sheep serum induced passive anaphylaxis model was carried out by wistar rats. The selected rats were administered intraperitoneally with serum of active anaphylactic rats. After 48 h all the rats are administered intraperitoneally with 1ml of sheep serum. The rats which are pre-treated with prednisolone (10 mg), polyherbal formulation (250 mg) were analyzed for the mast cell degranulation. Passive paw anaphylaxis model was carried out by subcutaneous administration of 0.1 ml serum of active anaphylactic rats into the plantar region of left hind paw of rats. After 24 h the left hind paw of rats was administered subcutaneously with 0.1 ml of egg albumin. The rats which are pre-treated with prednisolone (10 mg), polyherbal formulation (250 mg) were analyzed for the increase in paw volume. Antianaphylactic activity of polyherbal formulation on the mesenteric mast cells of the rat may be possibly due to the membrane stabilizing potential.

Antiallergic activity of different kinds of medicinal vegetative raw material

The aim of the study. To study 26 kinds of medicinal vegetative raw material to reveal their antianaphy lactic activity. Materials and methods. Comparative studying of antianaphylactic activity of vegetative objects was carried out with use of ovalbumin models of systemic anaphylactic shock and passive skin anaphylaxis. Results. All kinds of the investigated vegetative raw material had a suppressing action on the development and the degree of suppression the passive skin anaphylaxis. The most active were liquorices, leaves of a birch, cowberry, nettle, mint, walnut, tea green, St.-Gohn's wort, horsetail herb, hop cones, linden flowers, chamomile flowers, styles with stigmata of maydays. The influence of water extractions on the development of system anaphylactic shock has been shown, as well as some vegetative objects extended the time of beginning anaphylactic reactions and reduced the degree of their expressiveness. The most active were liquorices, leaves of birch, cowberry, nettle, mint, walnut, grass of violet, horsetail, hop cones and lindens flowers. The conclusion. Liquorices, leaves of birch, nettle, mint, walnut, tea green, grass of horsetail, violet, styles with stigmata of maydays possess antianaphylactic activity