A Novel Framework for Efficient Multiple Signature on Certificate with Database Security

PKI gives undeniable degree of safety by transferring the key pair framework among the clients. By constructing, a PKI we combine digital identities with the digital signatures, which give an end-to-end trust model. Basically, PKI is an attempt, which can simulate the real-world human analyzation of identity and reliability in a computerized fashion. In any case, the existing applications are centered on a tight trust model which makes them inadequate as an overall device for trust examination. After years of research, development and deployment, PKI still facing strong technical and organizational challenges such as attacks against Certificate Authorities (CA). CAs are the primitive component of PKIs which plays powerful role in the PKI model. CA must be diligent, creditable and legitimate. In any case, a technocrat who picks up control on a CA can use CA's certificate to issue bogus certificate and impersonate any site, such as - DigiNotar, GobalSign, Comodo and DigiCert Malaysia. In this paper we proposed an approach to reduce the damage of compromised CA/CA’s key by imposing Multiple Signatures (MS) after verifying/authenticating user’s information. One single compromised CA is not able to issue a certificate to any domain as multiple signatures are required. Private key and other perceptive information are stored in the form of object/blob. Without knowing the structure of class no one can access the object and object output stream. Proposed MS achieve better performance over existing MS schemes and control fraudulent certificate issuance with more database security. The proposed scheme also avoids MITM attack against CA who is issuing certificate to whom which is using the following parameters such as identity of Sender, Receiver, Timestamp and Aadhar number.

Alterations in the RB Pathway With Inactivation of RB1 Characterize Glioblastomas With a Primitive Neuronal Component

A primitive neuronal component is a feature of some glioblastomas but defining molecular alterations of this histologic variant remains uncertain. We performed next-generation sequencing of 1500 tumor related genes on tissue from 9 patients with glioblastoma with a primitive component (G/PN) and analyzed 27 similar cases from the Cancer Genome Atlas (TCGA) dataset. Alterations in the RB pathway were identified in all of our patients’ tumors and 81% of TCGA tumors with the retinoblastoma tumor suppressor gene (RB1) commonly affected. Although RB1 mutations were observed in some conventional glioblastomas, the allelic fractions of these mutations were significantly higher in tumors with a primitive neuronal component in both our and TCGA cohorts (median, 72% vs 25%, p < 0.001 and 80% vs 40%, p < 0.02, respectively). Further, in 78% of patients in our cohort, RB expression was lost by immunohistochemistry. Our findings indicate that alterations in the RB pathway are common in G/PNs and suggest that inactivation of RB1 may be a driving mechanism for the phenotype.