Resolving the spatial distributions of Dipturus intermedius and Dipturus batis—the two taxa formerly known as the ‘common skate’

Batoid fishes are among the most endangered marine vertebrates, yet conservation efforts have been confounded by incomplete taxonomy. Evidence suggest that the critically endangered ‘common skate’ actually represents two species: the flapper skate (Dipturus intermedius) and the blue skate (Dipturus batis). However, knowledge of the geographic range of these two nominal species is limited. Here, DNA sequencing is used to distinguish these species, allowing their spatial distributions to be clarified. These records were also used as the basis for species distribution modelling, providing the first broad scale models for each species across the Northeast Atlantic. Samples were obtained from Iceland, the UK (specifically Shetland), the North Sea and the Azores. Results suggest that D. batis was commonly distributed in the Western Approaches and Celtic Sea, extending out to Rockall and Iceland. D. intermedius generally appears to be less abundant, but was most frequent around northern Scotland and Ireland, including the northern North Sea, and was also present in Portugal. Two individuals were also identified from seamounts in remote areas of the Atlantic around the Azores, the furthest south and west the species has been found. This supports reports that the flapper skate historically had a much wider distribution (which was also highlighted in the distribution model), emphasising the large scale over which fisheries may have led to extirpations. Furthermore, these Azorean samples shared a unique control region haplotype, highlighting the importance of seamounts in preserving genetic diversity.

Anthropogenic invaders: historical biogeography, current genetic status and distribution range of the “peludo” Chaetophractus villosus (Xenarthra) in Patagonia and Tierra del Fuego, southern South America

Chaetophractus villosus was once distributed from the south of Bolivia and Paraguay to the northwest of Patagonia, where the species survived in glacial refugia during Pleistocene. After the ice retreated, the species expanded its distribution further south reaching Chubut River. In the last two centuries, the species colonized the rest of Patagonia on both sides of the Andes and was introduced in Tierra del Fuego (TDF) in 1982, where it expanded its distribution range along 484 km2 until 2005. A single mitochondrial Control Region haplotype (HC) was described across Argentine Patagonia based on the low number of samples. This lack of variability was exceptional and impressive, taking into account that the northern neighboring areas of distribution are considered ancestral due to the haplotype richness. Here, we added new field and genetic data and compiled recent bibliography to update the biogeography and phylogeography of the species in southern South America. To explain the unprecedent lack of genetic variability, we discussed three main aspects: a historical biogeographic aspect related to the natural barriers for the species, a strong stochastic component, and the possible adaptive advantages of the only mitochondrial lineage detected, whose colonization and acclimatization to the new environment were mediated by man. We also estimated that the current distribution range in TDF is about 8527 km2.

Genetic analysis of a population crash in brush-tailed rock-wallabies (Petrogale penicillata) from Jenolan Caves, south-eastern Australia

Although the theoretical effects of a severe reduction in effective population size (i.e. a bottleneck) are well known, relatively few empirical studies of bottlenecks have been based on extensive temporally spaced samples of a population both before and after a bottleneck. Here we describe the results of one such study, utilising the Jenolan Caves (JC) population of the brush-tailed rock-wallaby (Petrogale penicillata). When first sampled in 1985 (n = 20) the JC population comprised ~90 individuals. Subsequently the population crashed, and by 1992 only seven individuals remained. In 1996 the entire population (n = 10) was again sampled. Genetic diversity in the pre- and post-crash JC population was compared using 11 polymorphic microsatellite loci and PCR–SSCP analysis of the mitochondrial DNA control region. Only a single unique control region haplotype was detected in the pre- and post-crash JC population, although variant haplotypes were present in other P. penicillata populations. Of the 35 microsatellite alleles present in the pre-crash population, nine (26%) were lost during the bottleneck. The average number of rare alleles declined by 72%, allelic diversity was reduced by 30% and average heterozygosity declined by 10%. These observations are consistent with theoretical predictions. Additional analyses revealed that a P.�penicillata female at Wombeyan Caves was the only survivor of a 1990/91 reintroduction attempt using animals from JC. Of the microsatellite alleles detected in this female, 21% (4/19) were no longer present in the post-crash JC population. Furthermore, the genetic profiles of animals from the recently discovered Taralga population indicate that they are not derived from JC stock, but represent a threatened remnant of a hitherto undetected natural P. penicillata population.