Genetic Risks and Demographic History of Indigenous Goats of Mongolia Revealed by SNP Genotyping

Recent climatic disasters (dzudzs) and uncontrolled massive breeding endangered valuable ge-netic resources of Mongolian goats labored by five thousand years of evolution in extremes of Al-tai mountains and Gobi deserts. Meanwhile, Mongolian goats has never been characterized by genomic data. We used Illumina Goat SNP50 to investigate phylogenic relationships and genetic risks in 5 local Mongolian populations: Erchim (N=37) from geographically remote Darkhat Valley in the northern mountains, Ulgii Red (N=35) and Dorgon (N=28) from the western mountain region, Buural (N=34) from the western steppe and mountain areas and Gobi Gurvan Saikhan (N=33) from semi-arid steppe area in the South Gobi region. ROH analyses, estimated populations sizes and Fst values showed South Gobi and Darkhat Valley goats to be of a high risk of inbreed-ing that however appear to be of distinct origin (artificial selection vs geographical isolation). In-dices of genetic differentiations between Mongolian goats were relatively low compared to Euro-pean breeds. Meanwhile, we suggest direct comparison is not fully appropriate given that Mongo-lian breeds were not subjected to intense selection. Darkhat Valley goats were clearly differentiat-ed from other Mongolian breeds according to various types of analyses. Phylogenetic relationships within XXX breeds of the rest of the world plotted Mongolian goats between some Russian (Altai, Orenburg) and Chinese populations (Nanjiang and Qinggeli). Thus, present study (i) highlights demographic history Mongolian goats and (ii)provides unified SNP-data called for support deci-sions in conservational genetics. Finally, our work (iii) raises a question of how exactly these data should be compared to make objective choices.

Phylogeography of Myrica nana: the Wumeng Mountains as a natural geographical isolation boundary on the Yunnan-Guizhou Plateau

The Yunnan-Guizhou Plateau (YGP) is characterized by the distinctive isolated habitat of limestone Karst Islands and features the Wumeng Mountains (Mts), which divide the YGP into the two Plateaus of Yunnan and Guizhou. This study aims to assess the effects of past geographic and environmental isolation and climate fluctuation on the flora distribution in the YGP. To this effect, we analyzed the phylogeographical pattern and genetic structure for Myrica nana, a vulnerable species endemic to the YGP, based on chloroplast and nuclear ribosomal DNA sequence. The results suggest that the genetic and haplotype network structures are divided into at least two groups—cpDNA haplotype H2 (or nrDNA haplotypes h1, h2, and the native haplotype) mainly distributed to the east of the Wumeng Mts, and cpDNA haplotype H1 and haplotypes H3–H10 (or nrDNA haplotype h3) distributed to the west of the Wumeng Mts. A deep genetic split was noted within the two groups to reach 25 steps, especially for the cpDNA fragment variation. The east–west divergence reveals the existence of a natural geographical isolation boundary in the form of the Wumeng Mts, which divides the YGP into the Yunnan and Guizhou Plateaus. Therefore, there existed at least two glacial refugia during the Quaternary glacial period, along with a genetic diversity center, and at least two large geographic protection units for the vulnerable species of M. nana, distributed throughout the eastern and western sides of the Wumeng Mts. This study not only clarifies that the phylogeographical pattern and genetic structure for M. nana can be attributed to geographic and environmental isolation and climate fluctuation, but it also proposes an effective strategy to protect vulnerable species and the important wild flora of the YGP.

When and How Did Cannabis Reach Europe and the Iberian Peninsula?

Cannabis is among the oldest human domesticates and has been subjected to intensive artificial (human-mediated) selection through history, to create a wide array of varieties and biotypes for a diversity or uses, including fiber, food, biofuel, medicine and drugs, among others. This paper briefly reviews the available literature on the taxonomy, the evolutionary origin and the domestication of this plant, as well as its worldwide dispersal, either in its wild and cultivated forms. Emphasis is placed on Europe and especially on the Iberian Peninsula, which is the main target of this study. Today it is accepted that Cannabis is a monospecific genus with two subspecies, C. sativa subsp. sativa and C. sativa subsp. indica, originated in Europe and Asia, respectively, by allopatric differentiation after geographical isolation fostered by Pleistocene glacial-interglacial cycles. Palynological and phylogeographical evidence situate the Cannabis ancestor in the NE Tibetan Plateau during the Oligocene (ca. 28 Ma). The timing and place of domestication is still a matter of debate between contrasting views that defend single or multiple domestication centers, situated in different parts of the Eurasian supercontinent, notably central/southeastern China and the Caucasus region. Recent meta-analyses suggest that wild Cannabis may have been spread across Europe already in the Pleistocene (ca. 1 Ma), and its domestication could have been occurred during the European Copper/Bronze ages (7-5 kyr BP). According to the available reviews and meta-analyses, pre-anthropic dispersal of Cannabis into the Iberian Peninsula seems to have been occurred only in post-glacial times (18.5-15 kyr BP) and the earlier signs of cultivation date to the Early Medieval Ages (ca. 600 CE). However, the palynological and archaeological evidence used to date is insufficient for a sound assessment and the development of thorough Iberian databases to address further meta-analysis are essential for more robust conclusions. Some clues are provided for these achievements to be fulfilled.

Morphological and molecular characterization of Castniidae (Lepidoptera) associated to sugarcane in Colombia

The giant sugarcane borer, Telchin licus, has been reported as an economically important sugarcane pest in Colombia; however, its taxonomic status has been scarcely investigated and previous reports offer an ambiguous characterization of both the immature and adult stages. The objective of this work is to identify Telchin species affecting sugarcane and alternative hosts in different departments of the country by integrating molecular analysis and conventional morphology. To date, T. licus has been found in the departments of Caquetá, Casanare, and Meta, while T. atymnius has been found in Antioquia, Caldas, Nariño, and Valle del Cauca. Sugarcane, Musaceae, and Heliconiaceae have been found to be hosts to both species. Additionally, the species T. cacica has also been registered in the department of Nariño, affecting heliconias and plantains. Genetic variation within the species allowed differentiation at the molecular level of subspecies of T. licus and T. atymnius, confirming that the subspecies present in Colombia are T. licus magdalena, T. atymnius humboldti, and T. atymnius atymnius. The haplotype diversity of populations is closely related to their geographical distribution, indicating low gene flow between populations and possible speciation inside the country. Analysis of genetic variance showed significant differences among and within T. atymnius populations, which may suggest a high genetic structure along the regions where it is found and the possible presence of additional subspecies to those previously reported. To understand the geographical and environmental conditions that determine the pest's distribution in Colombia, this information needs to be complemented with ecological considerations of possible geographical isolation and association of alternative hosts.

Taxonomic notes on the Australian skyhopper genus Kosciuscola Sjöstedt (Orthoptera: Acrididae: Oxyinae)

The Australian skyhopper genus Kosciuscola Sjöstedt consists of brachypterous species that inhabit the Australian alpine and subalpine region. The genus used to include 5 species and 1 subspecies, but according to a recent phylogenomic study, there could be as many as 14 species in the genus, that are genetically and geographically isolated from each other. This study represents the first step in describing and documenting the diversity of this interesting genus. In this study, we redefine the type species K. tristis, and elevate its subspecies K. tristis restrictus as a valid species on the basis of distinct morphological traits, geographical isolation, and phylogenomic evidence.

Holocaust Consciousness in New Zealand 1980-2010: A Study

<p>This thesis chronicles and examines the major New Zealand specific Holocaust-related issues of the last three decades, in the time period 1980 to 2010. The Holocaust has had a long reaching legacy worldwide since the end of the Second World War. There have been major news items and issues that have brought the Holocaust to the forefront of people's consciousness throughout the decades, the most prominent example being the trial of Adolf Eichmann in 1961. It was major news such as that trial, as well as Hollywood productions such as the TV miniseries Holocaust in the late 1970s, that brought about widespread consciousness of the Holocaust worldwide, in countries such as the United States and Australia. In New Zealand, but major Holocaust-related issues connected specifically to New Zealand did not begin to emerge until the 1980s. This thesis investigates, in three chapters, differing issues over the aforementioned time period that have had an impact on consciousness of the Holocaust in New Zealand. The issues investigated are respectively: the war criminals investigation of the late 1980s and early 1990s, the colonial 'holocaust' argument of the late 1990s and early 2000s, Holocaust denial controversies in New Zealand academia, and the growth and evolution of Holocaust commemoration and education. Although some issues, such as commemoration and education, began earlier, it was not until the 1980s that these issues developed in earnest and a greater number of people began to take notice of the connection of these issues, and in turn New Zealand, to the Holocaust. The main arguments made in this thesis are that New Zealand's consciousness of the Holocaust developed when it did and at the rate it did because of particular aspects of the Jewish community and New Zealand society as a whole, including the geographical isolation of the country, the size and assimilation of the Jewish and survivor communities here, and the overall attitudes and on occasion apathy and ignorance towards the Holocaust. All of these aspects have influenced, to varying degrees, consciousness of the Holocaust within New Zealand throughout the time period of 1980 to 2010.</p>

Holocaust Consciousness in New Zealand 1980-2010: A Study

<p>This thesis chronicles and examines the major New Zealand specific Holocaust-related issues of the last three decades, in the time period 1980 to 2010. The Holocaust has had a long reaching legacy worldwide since the end of the Second World War. There have been major news items and issues that have brought the Holocaust to the forefront of people's consciousness throughout the decades, the most prominent example being the trial of Adolf Eichmann in 1961. It was major news such as that trial, as well as Hollywood productions such as the TV miniseries Holocaust in the late 1970s, that brought about widespread consciousness of the Holocaust worldwide, in countries such as the United States and Australia. In New Zealand, but major Holocaust-related issues connected specifically to New Zealand did not begin to emerge until the 1980s. This thesis investigates, in three chapters, differing issues over the aforementioned time period that have had an impact on consciousness of the Holocaust in New Zealand. The issues investigated are respectively: the war criminals investigation of the late 1980s and early 1990s, the colonial 'holocaust' argument of the late 1990s and early 2000s, Holocaust denial controversies in New Zealand academia, and the growth and evolution of Holocaust commemoration and education. Although some issues, such as commemoration and education, began earlier, it was not until the 1980s that these issues developed in earnest and a greater number of people began to take notice of the connection of these issues, and in turn New Zealand, to the Holocaust. The main arguments made in this thesis are that New Zealand's consciousness of the Holocaust developed when it did and at the rate it did because of particular aspects of the Jewish community and New Zealand society as a whole, including the geographical isolation of the country, the size and assimilation of the Jewish and survivor communities here, and the overall attitudes and on occasion apathy and ignorance towards the Holocaust. All of these aspects have influenced, to varying degrees, consciousness of the Holocaust within New Zealand throughout the time period of 1980 to 2010.</p>

A Cross-Sectional Study on Patient Satisfaction Regarding Teleconsultation Practice During the Covid-19 Pandemic in a Neuro-Psychiatric Hospital in South India

BACKGROUND Telemedicine involving teleconsultation has been a boon to people during this pandemic situation. Safeguarding one against the infection should not put people who are in dire need of medical help feel helpless and desperate. Teleconsultation, which was previously used for people who did not have access to medical help due to geographical isolation, has come to the rescue of people during this pandemic, and sometimes it is the only timely help that is available to people in need. We wanted to measure the patient satisfaction level regarding the use of telemedicine for psychiatric and psychological consultations during the Covid-19 pandemic. METHODS The level of patient satisfaction was measured using the patient experience and satisfaction survey’ proposed (The Research and Development Survey-RANDS) (measuring patient experience and satisfaction to telemedicine, 2020) to measure the level of satisfaction to telemedicine during this pandemic situation, regarding the use of telemedicine for patients who consulted doctors and psychologists in a psychiatric hospital in South India. People who participated filled the questionnaire through google form links sent to them. RESULTS The results indicate that 28 % of people who consulted psychiatrists, recorded scores indicating that they were satisfied with the teleconsultation service, while 71 % of people who consulted psychologists reported being highly satisfied with the consultation. This study indicated that some still preferred direct interaction with psychiatrists as evidenced by 72 % of people feeling dissatisfied with teleconsultation. CONCLUSIONS This study shows that with people struggling with mental illness direct interaction is crucial in the treatment process, however, teleconsultation could be used for emergencies to ensure compliance with medication and for monitoring symptoms in patients. KEY WORDS COVID 19, Teleconsultation, Neuro-Psychiatric Hospital, Patient Satisfaction

Spatial Genetic Structure of Prunus mongolica in Arid Northwestern China Based on RAD Sequencing Data

The extensive range of sand deserts, gravel deserts, and recent human activities have shaped habitat fragmentation of relict and endangered plants in arid northwestern China. Prunus mongolica is a relict and endangered shrub that is mainly distributed in the study area. In the present study, population genomics was integrated with a species distribution model (SDM) to investigate the spatial genetic diversity and structure of P. mongolica populations in response to habitat fragmentation and create a proposal for the conservation of this endangered species. The results showed that the northern marginal populations were the first isolated from other populations. The SDM suggested that these marginal populations had low levels of habitat suitability during the glacial period. They could not obtain migration corridors, and thus possessed low levels of gene flow connection with other populations. Additionally, several populations underwent secondarily geographical isolation from other central populations, which preserved particular genetic lineages. Genetic diversity was higher in southern populations than in northern ones. It was concluded that long-term geographical isolation after historical habitat fragmentation promoted the divergence of marginal populations and refugial populations along mountains from other populations. The southern populations could have persisted in their distribution ranges and harbored higher levels of genetic diversity than the northern populations, whose distribution ranges fluctuated in response to paleoclimatic changes. We propose that the marginal populations of P. mongolica should be well considered in conservation management.