Significance of the Sachs-Witebsky cytochol reaction for serodiagnosis of syphilis

Serology of syphilis, which has a relatively recent past (since 1907), in recent years has written into its pages a number of huge successes, which greatly facilitated the diagnosis of syphilis. Serology of syphilis owes such success to the so-called groove. flocculation reactions, the number of which is increasing every year, since despite the great successes in this area, still no other reaction has been proposed, in 100% of cases positive at different stages of syphilis and not possessing a groove. nonspecificity, i.e. the ability to give a positive result in the absence of syphilis.

Serodiagnosis of syphilis in pregnant women, Stühmer and Dreyer (Zeit. F. Gab., Bd. 84)

Serodiagnosis of syphilis in pregnant women, Sthmer and Dreyer (Zeit f. Gab., Bd. 84) note the unreliability of serological tests for syphilis in pregnant women and women in labor: due to metabolic disorders depending on the liver and placenta, approximately 10% of pregnancy is nonspecific. inhibition of reactions to lues.

On the question of the practical value of the Stamm antigen according to Kalinin and Ginsburg

Soon after the Wassermann reaction was introduced for the diagnosis of syphilis, aspirations appeared that had the goal, on the one hand, to improve the method of serodiagnosis of syphilis in the sense of obtaining more accurate results, since the Wassermann reaction, even with the most careful formulation, is still sometimes gave unsatisfactory results, on the other hand, a simplification of this technique, in order thereby to be able to apply serodiagnostic methods in primitive laboratory conditions, without laboratory animals.

To serodiagnosis of syphilis

Stiden (Bp. Delo, 1924, No. 10) of all sedimentary reactions proposed for the diagnosis of syphilis, especially recommends the Kahn reaction, which, on the one hand, is distinguished by the simplicity of technical implementation, and on the other hand, it is not inferior in accuracy and sensitivity to the Wassermann reaction. a The main extract for this reaction is prepared in the following way: a fresh cow heart turns into a cutlet mass, dried in a thermostat at 37 in open Petri cups and passed several times through a coffee mill, after which it is ground in a mortar into a fine powder

A retrospective observational comparative study of VDRL vs. TPHA and their role in serodiagnosis of syphilis in a tertiary care centre

<p class="abstract"><strong>Background:</strong> <span lang="EN-IN">Syphilis is difficult to diagnose challenging the clinicians. Combined use of both non treponemal and treponemal serological tests will give correct diagnosis of syphilis.</span>The aim of the study was <span lang="EN-IN">to evaluate the role of VDRL and TPHA in the serodiagnosis of syphilis.</span></p><p class="abstract"><strong>Methods:</strong> <span lang="EN-IN">Open label retrospective study done in the department of dermatology of a tertiary care centre after getting IEC approval. One hundred and seventy patients’ serological reports done for syphilis during the month January 2017 to May 2017 were taken for the study and were analysed retrospectively after categorizing into three groups. Frequency and percentage of patients who were reactive to VDRL, weakly reactive to VDRL, positive for prozone phenomenon and reactive to TPHA were noted in each group and analysed statistically</span>.<strong></strong></p><p class="abstract"><strong>Results:</strong> <span lang="EN-IN">In our study two cases showed biological false positive results in group 1 and three cases in group 3 were biological false reactors. In our study five patients in group 1 and three patients in group 3 were non-reactive to VDRL but were reactive to TPHA due to the presence of Ig G antibodies and all these patients gave a past history of treatment of syphilis. </span></p><p class="abstract"><strong>Conclusions:</strong> <span lang="EN-IN">Our study was successful in evaluating the role of TPHA and VDRL in the sero-diagnosis of syphilis. The VDRL and TPHA being simple and economical tests can be combined in the assessment of syphilis and ensures that no cases are missed.</span></p>