Design of Rapid Bacterial Identification System Based on Scattering of Laser Light and Classification of Binned Plots

The rapid detection and classification of bacterial pathogens have great medical utility, and widespread applications in clinical labs, intensive care units, and infectious disease departments. The Rapid Bacterial Identification System (RBIS) is based on the principle of variation in light scattering when the laser beam passes through bacterial microbes. The use of laser light scattering and classification of binned plots algorithm gives a new dimension for real-time identification of different pathogens without any biochemical processing. Bacterium identification device consists of an assembly of photodetectors surrounded by the bacterial sample at different angles. The photodetectors acquire the scattered light in form of peaks when the laser beam passes by the sample. The acquired peak values were used to create 3D histograms to evaluate the frequency of occurrence. However, the identification is based on creating two dimensional binned plots with the help of the frequency of occurrence of peak values across two photodetectors. The algorithm of the system consists of two parts: Library files and the Comparator. Library files contain data of bacterial species in form of binned plots, while comparator compares the data of test sample with library files. The classification of sample depends on the maximum resemblance of the number of binned plots with library files. The classification of Enterococcus faecalis, Staphylococcus aureus, and Escherichia coli gives mean accuracies of 81.8%, 70.9%, and 71.4 %, respectively. The proposed system can be applied to future real-time intelligent theranostic systems for diagnosis and treatment of pathogenic diseases.

A Guiding Design System for Pressure Vessels based on 3D CAD

The design of pressure vessel is an extremely professional job which has heavy workload, repeated work, and rich accumulated knowledge, and it has good characteristics of serialization, generalization, standardization. Generic 3D CAD systems are not suitable for pressure vessel design. On the contrary, customized development based on 3D CAD system is needed. In this paper, a new pressure vessel design system based on Chinese 3D CAD system named SINOVATION was developed to improve design efficiency and accuracy. First, the functions and construction of pressure vessel design system were presented with the analysis of the traditional design process of pressure vessels. Second, related design specifications were managed and used flexibly in pressure vessel design system, and general parts library and standard parts library were established to improve parts reuse and accumulation. Finally, a guiding design mode was accepted for typical vessels and components to design same product series while direct modeling function was used for atypical pressure vessels. The results show that this method is helpful for embedded use of design specifications, standards and knowledge, and also provides an extensive interface for product updates and diversification. Statistical data reports and accurate engineering drawings are generated automatically based on 3D models. The quality, efficiency, and consistence are greatly improved. With the implementation and application, the system has been highly commended by customers in Chinese pressure vessel industry, especially in petrochemical industry.