Application of Arbuscular Mycorrhiza from Senaru Forest Rhizosphere for Gyrinops versteegii Germination and Growth

The aims of this research are to apply Senaru forest rhizosphere on Kabupaten Lombok Barat West Nusa Tenggara as Mycorrhiza inoculants for Gyrinops versteegii germination and growth. Rhizosphere sample was taken from ten sampling spot on Senaru forest between Latitude: 08o18.808’ S – 08o19.174’ S and Longitude: 116o24.138’ E – 116o24.181’E. This study employed Factorial Experiment Design with 2 Factor including: Medium Composition (M) and Mychorizza Inoculant (I). There were 5 media composition: M1 (sand), M2 (soil:sand = 1:2), M3 (soil:sand = 1:1), M4 (soil:sand = 2:1), M5 (soil). There were 2 types of Inoculation: I1 (without inoculant) and I2 (innoculant from senaru Rhizosphere). Growth parameters observed in this study were: germination percentage, stem length, stem diameter and root colonization. Germination percentage of G. versteegii seeds in all growth media are below 60 % which could be classified as low germination rate. Also germination from media without rhizosphere is higher than germination from media with rhizosphere. On the other hand, G. versteegii growth on rhizosphere media is slightly higher than growth of G. versteegii on media without rhizosphere based on stem diameter and length measurement. It tends that medium composition with higher sand proportion tended to gives better germination and growth rate of G. verteegii. Myorrhiza colonization on G. versteegii root was higher in media with rhizosphere addition. It could be concluded that Application of Senaru rhizosphere containing Mycorrhiza increases G. versteegii growth but not its germination percentage. This research enrich knowledge in biological science about asociation of mycorrhiza with G. versteegii especially on its growth and germination

Chryseobacterium gallinarum sp. nov., isolated from a chicken, and Chryseobacterium contaminans sp. nov., isolated as a contaminant from a rhizosphere sample

Two yellow-pigmented bacterial strains (100T and C26T), showing 98.4 % 16S rRNA gene sequence similarity to each other and isolated from a chicken in Germany and as a contaminant from an agar plate of a rhizosphere sample in Alabama, were studied by using a polyphasic taxonomic approach. Cells of both isolates were rod-shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequences of the two organisms with the sequences of the type strains of the most closely related species of the genus Chryseobacterium showed the highest sequence similarities of strains 100T and C26T to the type strains of Chryseobacterium joostei (respectively 97.5 and 98.2 %), C. viscerum (96.6, 97.8 %), C. gleum (97.1, 97.7 %), C. arthrosphaerae (97.3%, 97.7 %), C. indologenes (97.2, 97.7 %), C. tructae (96.6, 97.6 %), C. jejuense (97.0, 97.6 %) and C. oncorhynchi (96.3, 97.5 %); 16S rRNA gene sequence similarities to members of all other species of the genus Chryseobacterium were below 97.5 %. The fatty acid profiles of both strains consisted of the major fatty acids iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C17 : 1ω9c and iso-C17 : 0 3-OH, but also showed slight differences (absence or presence of C16 : 0 3-OH and iso-C15 : 1 F). DNA–DNA hybridizations between the two strains and between the novel strains and the type strains of C. joostei , C. indologenes , C. jejuense , C. tructae and C. viscerum resulted in relatedness values clearly below 70 %. These DNA–DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed that both strains 100T and C26T represent novel species, for which the names Chryseobacterium gallinarum sp. nov. (type strain 100T = LMG 27808T = CCM 8493T) and Chryseobacterium contaminans sp. nov. (type strain C26T = LMG 27810T = CCM 8492T) are proposed.