scholarly journals Application of Arbuscular Mycorrhiza from Senaru Forest Rhizosphere for Gyrinops versteegii Germination and Growth

2018 ◽  
Vol 10 (2) ◽  
pp. 433-439 ◽  
Author(s):  
I Gde Adi Suryawan Wangiyana ◽  
Sukardi Malik
Keyword(s):  
Growth Parameters ◽  
Germination Rate ◽  
Germination Percentage ◽  
Medium Composition ◽  
Stem Diameter ◽  
Stem Length ◽  
Growth Media ◽  
Media Composition ◽  
Germination And Growth ◽  
Rhizosphere Sample

The aims of this research are to apply Senaru forest  rhizosphere on Kabupaten Lombok Barat West Nusa Tenggara as Mycorrhiza inoculants for Gyrinops versteegii germination and growth. Rhizosphere sample was taken from ten sampling spot on Senaru forest between Latitude: 08o18.808’ S – 08o19.174’ S and Longitude: 116o24.138’ E – 116o24.181’E. This study employed Factorial Experiment Design with 2 Factor including: Medium Composition (M) and Mychorizza Inoculant (I). There were 5 media composition: M1 (sand), M2 (soil:sand = 1:2), M3 (soil:sand = 1:1), M4 (soil:sand = 2:1), M5 (soil). There were 2 types of Inoculation: I1 (without inoculant) and I2 (innoculant from senaru Rhizosphere). Growth parameters observed in this study were: germination percentage, stem length, stem diameter and root colonization. Germination percentage of G. versteegii seeds in all growth media are below 60 % which could be classified as low germination rate. Also germination from media without rhizosphere is higher than germination from media with rhizosphere. On the other hand, G. versteegii growth on rhizosphere media is slightly higher than growth of G. versteegii on media without rhizosphere based on stem diameter and length measurement. It tends that medium composition with higher sand proportion tended to gives better germination and growth rate of G. verteegii. Myorrhiza colonization on G. versteegii root was higher in media with rhizosphere addition. It could be concluded that Application of Senaru rhizosphere containing Mycorrhiza increases G. versteegii growth but not its germination percentage. This research enrich knowledge in biological science about asociation of mycorrhiza with G. versteegii especially on its growth and germination

scholarly journals Germination and seedling growth response of mango (Mangifera Indica L.) cultivars to different nursery potting media

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Gebresmon Gebregiorgs ◽  
Negasi Tekeste ◽  
Berhan Mengesha
Keyword(s):  
Seedling Growth ◽  
Growth Response ◽  
Growth Parameters ◽  
Germination Percentage ◽  
Stem Diameter ◽  
Root Number ◽  
Top Soil ◽  
Shoot Number ◽  
Potting Media ◽  
Germination And Growth

Abstract Background Mango is an important cash crop greatly contributing for food security of fruit growers in northwestern parts of Tigray region, northern Ethiopia. However, information was critically lacking with respect to germination and growth response of mango rootstock to varying compositions of growing media in the region. Thus, an experiment was conducted during 2018 to evaluate germination and seedling growth response of mango cultivars to different combinations of nursery potting media in a net house at Indasellassie. Seeds of Local, Dodo and Keitt cultivars of mango were sown in full top soil (M1); top soil: sawdust: sand (3:2:1)(M2); top soil: FYM (Farmyard manure): sand (3:2:1)(M3); and top soil: FYM: sawdust: (3:2:1)(M4). Randomized complete block design in a factorial arrangement with three replications were used. Data on germination and growth parameters were collected and analyzed using GenStat software. Results The findings of the experiment revealed that mango cultivar had significantly affected most of the germination and growth parameters except shoot number. Moreover, potting media affected most of the parameters except days to germination, germination percentage, stem diameter, root number, and shoot number. Interaction effects of cultivar and growing media significantly affected all parameters except internode length, shoot number and stem diameter. Accordingly, interactions of local mango cultivar and M3 media combination gave the highest for most of the parameters with the order of M3 = M1 = M4 > M2 for germination percentage, M3 = M1 > M4 = M2 for plant height, M3 > M1 > M2 = M4 for leaf number, M3 > M1 > M4 > M2 for leaf area, M3 = M1 > M2 = M4 for stem diameter, M3 = M1 > M2 > M4 for root number, M3 > M2 > M1 = M4 for root length, M3 > M1 > M2 = M4 for fresh weight, M3 > M1 = M2 = M4 for dry weight and Vigorosity II of mango. Conclusion Considering better mango performance on seed germination, seedling growth and establishment in the study area, mango growers should use Local mango cultivar with soil potting media combinations of top soil: FYM: sand in the ratio of 3:2:1 for improving productivity and food security.

Development of an embryo germination protocol for shy-seeded grape (Vitis vinifera L.)

2021 ◽  
pp. 1-9
Author(s):  
Ashwini P. Benke ◽  
Ram Krishna ◽  
Roshni R. Samarth ◽  
Shweta S. Dhumal ◽  
Waquar A. Ansari ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Germination Rate ◽  
Germination Percentage ◽  
Vitis Vinifera L ◽  
Growth Media ◽  
Embryo Germination ◽  
Indole Butyric Acid ◽  
Grape Berries ◽  
Benzyl Amino Purine

Abstract Acquisition and germination of seeds are the most desired targets for the improvement of vegetatively propagated crops. In the present study, we developed a potential embryo germination protocol for the Red Globe grape cultivar having a low seed germination rate. Three grape berries at different developmental stages, viz. 50, 60 and 70 days after flowering (DAF), were selected for in-vitro embryo germination. Three growth media, namely Emershad and Ramming (ER), Nitsch and Nitsch (NN) and Murashige and Skoog (MS), and plant growth regulators (benzyl amino purine (BA), 0.5, 0.7 and 0.9 mg/l; indole butyric acid (IBA), 1.0, 1.5 and 2.0 mg/l; and gibberellic acid (GA), 0.1, 0.3 and 0.9 mg/l) were screened individually in different combinations with three amino acids, namely cysteine, glutamine and proline (2.0 μmol/l each). The maximum embryos germination percentage recorded at 70 DAF was 63.33, 47.78 and 45.56% in ER, NN and MS media, respectively, supplemented with 0.9 mg/l BA, 2.0 mg/l IBA, 0.9 mg/l GA and 2.0 μmol glutamine. Glutamine was found to have the most significant impact, and it improved the rescued embryos germination. The present study provides a potential recipe for a medium that can facilitate efficient germination of grape embryos.

scholarly journals ALLELOPATHIC POTENTIAL OF WEED SPEICES AGERATUM CONYZOIDES L. AND CLEOME VISCOSA L. ON GERMINATION AND GROWTH OF SESAMUM INDICUM L

2015 ◽  
Vol 2 (2) ◽  
pp. 114-117
Author(s):  
Anbarasan R ◽  
Prabhakaran J
Keyword(s):  
Growth Parameters ◽  
Dry Weight ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Sesamum Indicum ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Allelopathic Potential ◽  
Germination And Growth ◽  
Cleome Viscosa

Various concentrations (5%, 10%, 15% and 20%) of aqueous extracts prepared from two weed species namely Ageratum conyzoides L. and Cleome viscosa L. and used for the present experiments to determine their allelopathic potential on growth and developmental changes on sesame (Sesamum indicum L.). The weed extracts showed an inhibitory effect on germination percentage, root and shoot growth, and fresh and dry weight of sesame seedlings. The extracts of A.conyzoides had more inhibitory effect at 20%concentration,than that of C.viscosa on growth parameters of sesame.

scholarly journals ASSESSMENT OF COMPOST QUALITY OF SERICULTURE WASTE ON THE GROWTH OF Anogeissus leiocarpus USING TWO COMPOSTING METHODS

2020 ◽  
Vol 1 (1) ◽  
pp. 32-46
Author(s):  
C. Kambai ◽  
R. O. Olatunji ◽  
M. J. Francis
Keyword(s):  
Mineral Composition ◽  
Growth Parameters ◽  
Germination Rate ◽  
Germination Percentage ◽  
Proximate Analysis ◽  
Management Problem ◽  
Higher Plant ◽  
Compost Quality ◽  
Anogeissus Leiocarpus

This study investigated the compost quality of sericulture waste on the growth of Anogeissus leiocarpus using two composting methods. The two-method adopted were M1 (pile method) and M2 (bin method). Sericulture waste which includes mulberry leaves and silkworm excreta were air dried for 24hours, measured (1 kg) and set in layers for both methods adopted. Samples of compost from each method were taken before planting to the laboratory for proximate analysis. Descriptive statistics and analysis of variance (ANOVA) were used to analyze the data obtained. Anogeissus leiocarpus seeds were soaked for 10 minutes at 35oC and at 9.5oC for 24 hours to enhance the breaking of its dormancy. Anogeissus leiocarpus seeds was planted using sericulture compost from both adopted methods. Result shows that M1 had the highest mineral composition. M1 recorded the highest (N %) with the value of 0.93%; (P %) with 0.089%; (K %) with 0.58%; (Ca %) with the value of 1.78% and (Mg %) with the value of 0.72% while M2 had a lower mineral composition. The result obtained for the early growth parameters shows that M1 had a higher plant height and stem girth which was significantly different from M2, the mixture of the two compost had the highest leaf count which was also significant while the control recorded the lowest value for all the parameters assessed. This result suggests that pile composting method is the best method for composting sericulture waste and can be used as a good and preferable alternative for inorganic manure in raising seedlings and trees species.  Adopting the use of sericulture waste as compost will help in reducing the waste management problem, enhance economic use of farm waste and also help to ease the low germination rate and germination percentage of Anogeissus leiocarpus. Kambai, C. | Department of Forestry Technology, Federal College of Forestry, Jos, Plateau state, Nigeria

scholarly journals Physiological Assessment of Radiation and PVP/ Zn-Nanoparticles on Sour Orange Seedling

2019 ◽  
pp. 1-18
Author(s):  
Nadia A. Hamed ◽  
M. Salah ◽  
M. F. Ahmed ◽  
Tahsin Shoala
Keyword(s):  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Tap Water ◽  
Protein Pattern ◽  
Polymer Network ◽  
Chemical Properties ◽  
Germination Percentage ◽  
Stem Diameter ◽  
Stem Length ◽  
Sour Orange

Aims: The objective of this study is evaluate the effects of both pre-sowing gamma irradiation at low dose of 0 or 20 Gy and the soaking part of radiated seeds in zinc nanoparticles (Zn NPs) solutions at rate 0, 500, 1000 and 2000 ppm on behavior and physiological changes of sour orange seedling. Study Design: The design of the study is Two-Way Randomized Blocks. Place and Duration of Study: The present study was carried out during two successive seasons of 2016-2017 and 2017-2018 on sour orange rootstock (Citrus aurantium) grown at the experimental farm of the  Horticulture Institute, Giza , Egypt. Methodology: Eight different treatments were used as follow: 1) Control, 2) Gamma radiation at 0 Gy + soaking in Zn NPs at 500 ppm. 3) Gamma radiation at 0 Gy + soaking in Zn NPs at 1000 ppm. 4) Gamma radiation at 0 Gy + soaking in Zn NPs at 2000 ppm. 5) Gamma radiation at 20 Gy + soaking in tap water Zn NPs at 0 ppm. 6) Gamma radiation at 20 Gy + soaking in Zn NPs at 500 ppm. 7) Gamma radiation at 20 Gy + soaking in Zn NPs at 1000 ppm. 8) Gamma radiation at 20 Gy + soaking in Zn NPs at 2000 ppm. Results: Transmission electron microscope (TEM) images showed multiple shapes and uniform distribution of Zn NPs through the polymer network and the mean size of Zn NPs ranging is 40.9 nm. Generally, the results reveal that, 20 Gy combined with 1000ppm Zn NPs treatment increased seed germination percentage and stem length. While, 20 Gy plus 500 ppm Zn NPs treatment increased stem diameter and plant pigments concentration. Protein analysis of sour orange seedling treated with gamma radiation and then Zn NPs showed that, protein groups pattern (10-20 KD) enhanced with gamma rays 20 gray alone or with Zn NPs at 500 or 1000 ppm, while the protein groups pattern above 60 KD disappeared. Conclusion: Using gamma irradiation and then soaking part of irradiated seeds in Zn NPs solutions had significant effects on vegetative growth and root characters as well as some chemical properties of sour orange leaves. In addition, there are variable differences in the protein pattern between treated and untreated sour orange seedlings with gamma radiation and / or Zn NPs. Although, 20 Gy + 500 ppm Zn NPs treatment increased stem diameter, which is the main characteristic for the success of grafting process, the obtained results showed changes in proteins and it possible the gene structure had been changed due to this treatment.

scholarly journals Germination and growth of Albizia niopoides (Bentham) Burkart (Fabaceae)

2018 ◽  
Vol 40 (1) ◽  
pp. 39073
Author(s):  
Caroline Barbeiro ◽  
Taysi Pereira Firmino ◽  
André Henrique Oler de Novais ◽  
Mariza Barion Romagnolo ◽  
Lindamir Hernandez Pastorini
Keyword(s):  
Deciduous Forest ◽  
Growth Parameters ◽  
Dry Mass ◽  
Germination Percentage ◽  
Stem Height ◽  
Subsequent Growth ◽  
Pioneer Plant ◽  
Significant Difference ◽  
Germination And Growth

 Albizia niopoides (Bentham) Burkart, known as farinha seca, is an arboreal, deciduous and pioneer plant that can be found in the semi-deciduous forest in the Paraná basin. The study on the germination and growth of the plant is fundamental for better knowledge about the establishment and regeneration of the species. Aiming to verify the germination of A. niopoides and subsequent growth, green seeds and brown seeds were germinated in a germination chamber and growth parameters were evaluated at 60, 90, and 180 days after transplanting (DAT) in a greenhouse. There was no significant difference in germination percentage between green and brown seeds. However, lower levels of mean germination times and higher levels of germination speed index were observed for brown seeds. At 180 DAT, plants showed higher shoot dry mass, root dry mass, total dry mass, number leaves and height, with no significant difference between 60 and 90 DAT for total dry mass, root dry mass and root length. The values of stem height/diameter and Dickson quality index, associated to the other growth parameters, indicate quality of the seedlings for field planting at 180 DAT. 

scholarly journals Utilization of Grape Fruit Waste-based Substrates for Seed Germination and Seedling Growth of Lemon Basil

2017 ◽  
Vol 27 (4) ◽  
pp. 523-529 ◽  
Author(s):  
Mohammed El-Sayed El-Mahrouk ◽  
Yaser Hassan Dewir ◽  
Salah El-Hendawy
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Low Cost ◽  
Germination Rate ◽  
Germination Percentage ◽  
Waste Recycling ◽  
Grape Fruit ◽  
Fruit Waste ◽  
One To One

Grape (Vitis vinifera) waste management is a major problem in juice production, but it could be transformed into a major opportunity if the waste was recycled and used as a nursery growing medium. The aim of this study was to evaluate the suitability of four composts based on squeezed grape fruit waste (SGFW), mixed with coir or vermiculite in a one-to-one ratio by volume to form 13 growing media, for seed germination and seedling growth of ‘Mrs. Burns’ lemon basil (Ocimum basilicum var. citriodora). The final germination percentage (FGP), corrected germination rate index (CGRI), survival percentage, and seedling growth of ‘Mrs. Burns’ lemon basil were the variables measured. Pure SGFW reduced seed germination and seedling growth. The medium combining pure SGFW with vermiculite in a one-to-one ratio by volume was optimal for seed germination and seedling growth; in this medium the highest FGP, CGRI, survival rate, and growth parameters were recorded. The negative effects of pure SGFW composts were eliminated by mixing all composts with coir or vermiculite. These waste recycling media are low-cost products that can be beneficially used in nurseries on a commercial scale.

scholarly journals BIO-HERBICIDAL EFFICIENCY OF WITHANIA SOMNIFERA AGAINST IMPORTANT HIMALAYAN WEEDS

2017 ◽  
Vol 9 (3) ◽  
pp. 88
Author(s):  
Mamta Sharma ◽  
Rupinder Kaur ◽  
Sunil Puri
Keyword(s):  
Weed Management ◽  
Root Length ◽  
Withania Somnifera ◽  
Growth Parameters ◽  
Chenopodium Album ◽  
Foliar Spray ◽  
Dry Weight ◽  
Stem Length ◽  
Fresh Weight ◽  
Germination And Growth

Objective: The aim of the study was to evaluate the bioherbicidal activity of Withania somnifera against germination and growth of noxious weeds of Himalaya i.e. Ageratum coenyzoides, Chenopodium album and Achyranthus aspera in a laboratory and foliar spray bioassay which will act as a tool of weed management in the Himalayan region.Methods: Root, stem and leaf aqueous extract of Withania somnifera at 100% concentration were applied to determine their effect on the seed germination and seedling growth of tested weeds under laboratory condition. Foliar spray bioassay was carried out by spraying extracts of Withania somnifera on three-month-old seedlings of weeds for two months. Germination and growth of weeds were assessed using growth parameters into account i.e. germination percentage, stem length, root length, fresh weight, dry weight, no of shoot branches and no of root branches.Results: The results revealed that all the aqueous extracts markedly suppressed germination and seedling growth of A. coenyzoides, C. album and A. aspera. Withania extracts remarkably effected germination, the growth of Ageratum coenyzoides in comparison to other studied weeds. The leaf extract of Withania was more pronounced than stem and root extracts in germination assay. Infoliarspray bioassay, aqueous leaf extracts of 100% w/v concentrations were sprayed on three month old seedlings of weeds at an interval of 5 d. All the aqueous extracts significantly suppressed shoot length, root length, fresh weight and dry weight of Ageratum coenyzoides, Chenopodium album and Achyranthus aspera seedling. In foliar spray bioassay, leaf extract suppressed the stem length, root length, fresh weight, dry weight, no. of root branches and no of shoot branches of studied weeds more commendably than stem and root extract. The suppression of growth parameters could be attributed to the presence of phytochemicals present in the extracts of Withaniasomnifera.Conclusion: The present study concludes that Withania somnifera contain bio-herbicidal compounds in leaf, stem and root as plant extracts adversely affect weed growth of studied weeds. Henceforth the strategy bio-control of weed is a safe and effective method which could prove a milestone in weed management in Mid-Himalaya.

scholarly journals Pengaruh Posisi Skarifikasi Benih dan Perendaman Air Kelapa terhadap Perkecambahan Biji dan Pertumbuhan Bibit Sirsak (Annona muricata L.)

1970 ◽  
Vol 5 (1) ◽  
pp. 104-112
Author(s):  
Arya Agung Pranata ◽  
Asil Barus ◽  
Meiriani
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Coconut Water ◽  
Hard Seed ◽  
Randomized Block Design ◽  
Long Time ◽  
Germination And Growth

The problem of generative propagation of soursop is its seeds take a long time to germinate which are caused by the hard seed coat. The solution to solve that problem is scarification and coconut water soaking to simply the process of imbibition and seed germination and also stimulate the growth of shoot. This objective of the research was to determine the effect of the scarification position and the soaking with various concentrations of coconut water on seed germination and growth of soursop seedling. This research was held at the Faculty of Agriculture field, the University of Sumatera Utara in March - July 2017. The research used a factorial randomized block design with two factors and three replication. The first factor is the scarification position (without scarification, stomach scarification, top scarification) and the second factor is coconut water soaking (0%; 25%; 50%; 75 %). The result of the research showed that the germination rate parameter, germination percentage, crop height, number of leaves, root volume and dry weight of crop significantly higher on without scarification treatment than with scarification treatment. The interaction between the scarification position and soaking with various coconut water concentrations was not significantly affecting the seed germination and growth of soursop seedling.

scholarly journals PENGARUH MACAM SETEK DAN MEDIA TUMBUH TERHADAP VIGOR BIBIT KEMUKUS (Piper cubeba LINN)

2020 ◽  
Vol 12 (2) ◽  
pp. 67
Author(s):  
ENDJO DJAUHARIYA ◽  
MONO RAHARDJO ◽  
AGUS SUDIMAN ◽  
SUKARMAN SUKARMAN
Keyword(s):  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Growth Media ◽  
Pepper Plant ◽  
Media Composition ◽  
Rooted Cuttings ◽  
Two Factors ◽  
Medicinal Crops ◽  
Number Of Leaves

ABSTRAK<br />Tanaman kemukus (Piper cubeba LINN.) sudah dikenal sejak<br />jaman dahulu sebagai tanaman obat, rempah, pengharum dan penyedap<br />masakan. Di Jawa Tengah perbanyakan tanaman kemukus pada umumnya<br />dilakukan melalui setek panjang yang terdiri dari 8 - 14 ruas. Perbanyakan<br />dengan cara demikian dianggap tidak ekonomis, oleh karena itu perlu<br />dicari cara perbanyakan yang efisien dan efektif. Percobaan pengaruh<br />macam setek dan komposisi media tumbuh terhadap daya tumbuh dan<br />vigor bibit dilakukan dengan tujuan untuk mendapatkan teknologi<br />perbanyakan kemukus. Percobaan dilakukan di Kebun Percobaan<br />Cimanggu, Balai Penelitian Tanaman Rempah dan Obat Bogor dari bulan<br />September sampai dengan Desember 2003. Percobaan menggunakan<br />rancangan acak kelompok yang disusun secara faktorial dengan 2 faktor<br />dan 3 ulangan. Sebagai faktor pertama adalah 3 macam setek pendek 3<br />ruas yaitu : (1) setek bertapak, (2) setek sulur panjat dan (3) setek cabang<br />buah. Faktor kedua adalah tiga perlakuan komposisi media tumbuh terdiri<br />dari (tanah + pupuk kandang + pasir) dengan perbandingan: (a) 1:1:1, (b)<br />2:1:1, dan (c) 3:1:1. Media dimasukkan ke dalam polibag ukuran 10 x 12<br />cm. Variabel yang diamati meliputi persentase daya tumbuh, panjang<br />tunas, jumlah daun, bobot kering tunas, jumlah akar, panjang akar dan<br />bobot kering akar. Hasil percobaan menunjukkan bahwa vigor bibit yang<br />diekspresikan oleh persentase daya tumbuh, pertumbuhan tunas dan akar<br />tidak nyata dipengaruhi oleh interaksi jenis setek dan komposisi media<br />tumbuh. Jenis setek berpangaruh nyata terhadap semua variabel yang<br />diamati, kecuali terhadap jumlah daun. Jenis setek yang berasal dari setek<br />bertapak dan sulur panjat manghasilkan persentase daya tumbuh 68,40%<br />dan 62,00%, panjang tunas 2,87 cm dan 4,70 cm, bobot kering tunas 0,13<br />g dan 0,14 g, jumlah akar 5,95 dan 5,76 dan bobot kering akar 0,05 g dan<br />0.05 g, lebih baik dibandingkan setek cabang buah. Jenis media tumbuh<br />hanya berpengaruh nyata terhadap bobot kering tunas tapi tidak<br />berpengruh nyata terhadap variabel lainnya. Bobot kering tunas yang<br />terbaik didapat pada komposisi media tumbuh tanah + pupuk kandang +<br />pasir (1 : 1 : 1) (0,14 g) dan terendah pada komposisi media tumbuh tanah<br />+ pupuk kandang + pasir (3 : 1 : 1) (0.11 g).<br />Kata kunci : Kemukus, Piper cubeba LINN, bahan tanaman, macam<br />setek, media tumbuh, daya tumbuh, Jawa Barat<br />ABSTRACT<br />Effect of cutting materials and growth media on the<br />growth of cubeba cuttings<br />In Indonesia, cubeba pepper plant (Piper cubeba LINN) has been<br />known for years as a traditional medicine, spice, fragrant, and seasonings.<br />In Central of Java, it is usually propagated by using eight or fourteen node<br />cuttings which is not an economical practice. The research on cutting<br />materials and growth media was conducted in Cimanggu Experimental<br />Garden of the Indonesian Spice and Medicinal Crops Research Institute<br />from September to December 2003. The objective of the research was to<br />find out an appropriate propagation technology of cubeba. The research<br />used two factors and three replications which was arranged in a<br />randomized completely block design. The first factor was three kinds of<br />cutting nodes, i.e. (1) attached-rooted cuttings (2) vegetative branch and<br />(3) generative branch. The second factor was three kinds of media<br />compositions of soil, dung manure and sand (1) 1:1:1, (2) 2:1:1 and (3)<br />3:1:1. Observations were conducted on the percentage of budding, length<br />of bud, number of leaves, number of roots, length of root, dry weight of<br />the roots, and the shoot. The results of the research indicated that the vigor<br />of seedlings which was expressed by germination percentage, growth of<br />seedlings, and growth of root, did not significantly affected by the<br />interaction between kinds of cuttings and media composition. However,<br />the kinds of cuttings significantly affected all variables, except the number<br />of leaves. Cubeba seedlings originated from attached-rooted cuttings and<br />vegetative branch had higher germination percentage i.e. 68.40% and<br />62.00%, length of shoot 2.87 cm and 4.70 cm, dry weight of shoot 0.13 g<br />and 0.14 g, number of roots 5.95 and 5.76, length of root 7.32 cm and 7.27<br />cm, and dry weight of root 0.05 g and 0.05 g, compared to the cubeba<br />seedlings originated from generative branch. Media composition was<br />significantly effected only on dry weight of shoots. The highest dry weight<br />of shoot was resulted from composition of soil, dung manure and sand<br />1:1;1 (0.14 g), while the lowest was found on ratio media composition of<br />soil, dung manure and sand 3:1:1 (0.11g).<br />Key words : Cubeba, Piper cubeba LINN, plant material, cutting<br />materials, growth media, growth, West Java

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