Unravelling the mechanistic details of metal binding to mammalian metallothioneins from stoichiometric, kinetic, and binding affinity data

Equilibrium binding constants are now readily accessible for metalation of metallothioneins.

Non-coordinative metal selectivity bias in human metallothioneins metal–thiolate clusters

Mammalian metallothioneins MT-2 and MT-3 contain two metal–thiolate clusters through cysteine coordination of d10 metals, Cu(i) and Zn(ii), and isoform-specific non-coordinating residues control their respective zinc– and copper–thionein character.

Chemistry of mammalian metallothioneins and their interaction with amyloidogenic peptides and proteins

Tutorial focusing on the chemistry of mammalian metallothioneins, important to understand its biological functions in zinc and copper metabolism, detoxification and oxidative stress.