Evaluation of the Role of Na+/ K+ ATPase Ion Transporters and Na+/K+/ 2Cl and Na+/H+ Exchanger Cotransporters in Nephrons of Periophthalmus Waltoni Using Immunohistochemistry and Histology

Kidneys play an important role in regulating the balance of water and ions in freshwater and seawater fish. However, complex kidney structures impair a comprehensive understanding of kidney function. In this study, in addition to renal histology, Na+/K+/ATPase ion transporter proteins and Na+/K+/2Cl− and NHE3 cotransporters were located in Priophthalmus waltoni kidney tissue to evaluate the ion regulation abilities of epithelial cells in various parts of nephrons. The renal tubules are composed of proximal tubules and distal tubules, followed by collecting tubes and finally collecting ducts. Light microscope immunohistochemistry was utilized to locate Na+/ K+-ATPase along renal tubules and collecting ducts. However, the distribution of the Na+/K+-ATPase immune response varies in different sections. Na+/K+/CL− cotransporter positioning was reported only in collecting tubes and collecting ducts, and proximal tubes and distal tubes did not respond to Na+/K+/Cl− cotransporter immunolocalization. Immunohistochemical response for NHE3 localization was detected only at the apex of epithelial cells of proximal tubules and collecting tubes. The distal tubes showed negative reaction and the collecting ducts showed a weak response to NHE3 safety immunolocalization.

Otolith measurements to estimate standard length of gobiid fish (Teleostei: Gobiiformes) from the Persian Gulf and Oman Sea

The relationship between morphometric characters (length, weight and height) of otolith and fish standard length was determined for six intertidal gobiid species from the northern Persian Gulf and Oman Sea. A total of 144 sagittal otoliths were extracted and anlysed from Periophthalmus waltoni, Boleophthalmus dussumieri, Scartelaos tenuis, Istigobius ornatus, Bathygobius meggitti and Trypauchen vagina. Regression equations were derived and found that there is no significant difference in regression coefficients between left and right otoliths of the species studied. Results of the study showed that otolith length (OL) and height (OH) could estimate standard length in all the species studied except in T. vagina, where the otolith weight gave the best estimate of standard length. Results clearly indicated that otolith dimensions of the gobiid fishes studied could provide information about the species and size.

Haematological, blood biochemical and immunological responses to gradual acclimation to low-salinity water in Walton’s mudskipper Periophthalmus waltoni Koumans, 1941 (Perciformes: Gobiidae)

The present study investigates and reports the effects of gradual acclimation to low salinity water on some haematological, biochemical and immunological responses in Walton’s mudskipper, Periophthalmus waltoni. For this purpose, mudskippers caught from Persian Gulf coastal area (Bandar Khamir, Hormozgan Province, Iran) were maintained in laboratory aquaria with half seawater (50% SW, 17 ppt) and fed daily with frozen blood worms (Chironomus spp.) for one month prior to the start of experiments. After acclimation, groups of 18 individuals were either directly transferred to 50% SW (control), or acclimated to low salinity water during two sub-periods. In the first sub-period, fish were exposed to low salinity water namely to a gradual water salinity decrease of 1 ppt per day (during 17 days) until the final salinity of 0.4 ppt was reached. Afterwards, fish continued to maintain in this point of salinity (0.4 ppt), for further 15 days until day 32 (second sub-period). Fish were sampled on day 0, 17 and 32. Statistical analysis showed a significant influence of reduced salinity on erythrocytes, haemoglobin, haematocrit, leukocytes, lymphocytes, neutrophils, monocytes and on all biochemical and immunological parameters tested on day 17. However, these indices returned to the control level on day 32. Based on results, the extremely euryhaline p. waltoni can be acclimated to freshwater medium without showing any health disturbance if a gradual decrease in salinity is carried out for a long period of time.