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2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Michael Tessler ◽  
Jean P. Gaffney ◽  
Anderson G. Oliveira ◽  
Andrew Guarnaccia ◽  
Krista C. Dobi ◽  
...  

Abstract Pyrosomes are tunicates in the phylum Chordata, which also contains vertebrates. Their gigantic blooms play important ecological and biogeochemical roles in oceans. Pyrosoma, meaning “fire-body”, derives from their brilliant bioluminescence. The biochemistry of this light production is unknown, but has been hypothesized to be bacterial in origin. We found that mixing coelenterazine—a eukaryote-specific luciferin—with Pyrosoma atlanticum homogenate produced light. To identify the bioluminescent machinery, we sequenced P. atlanticum transcriptomes and found a sequence match to a cnidarian luciferase (RLuc). We expressed this novel luciferase (PyroLuc) and, combined with coelenterazine, it produced light. A similar gene was recently predicted from a bioluminescent brittle star, indicating that RLuc-like luciferases may have evolved convergently from homologous dehalogenases across phyla (Cnidaria, Echinodermata, and Chordata). This report indicates that a widespread gene may be able to functionally converge, resulting in bioluminescence across animal phyla, and describes and characterizes the first putative chordate luciferase.



2019 ◽  
Vol 112 (4) ◽  
pp. 1902-1911 ◽  
Author(s):  
Jong-Seok Park ◽  
Claudia Husseneder ◽  
Robert L Schlub

Abstract Ironwood trees (Casuarina equisetifolia subsp. equisetifolia L.) are ecologically and economically important trees in tropical and subtropical regions of the Indo-Pacific. Ironwood is one of the dominant tree species in Guam, but since 2002, this tree has been declining dramatically. A previous study showed that numerous sick or dead trees were under termite attack. However, the species of termites were not identified. As a first step to investigate causal relationships between termites and ironwood tree death, we assigned termites collected from ironwood trees to species using a combination of morphological characters and DNA barcoding of the 12S, 16S, COI, COII, and ITS2 regions. Based on morphology and comparisons to reference sequences in NCBI GenBank, the most likely species assignments were Nasutitermes takasagoensis (Nawa) (Blattodea: Termitidae) found to infest 45 trees, followed by Coptotermes gestroi (Wasmann) (Blattodea: Rhinotermitidae) (2 trees), Microcerotermes crassus Snyder (Blattodea: Termitidae) (2 trees), and an additional unidentified Microcerotermes species (1 tree) with no close sequence match to identified species in NCBI GenBank. However, taxonomic revisions and broader representation of DNA markers of well-curated specimen in public databases are clearly needed, especially for the N. takasagoensis species complex.



2014 ◽  
Vol 32 (2) ◽  
pp. 524-535 ◽  
Author(s):  
Florian Massip ◽  
Michael Sheinman ◽  
Sophie Schbath ◽  
Peter F. Arndt


Author(s):  
Tao Gao ◽  
Zhenjing Yao

The spectrum matching and correlation characteristic are both important in the multiple-user ultrasonic ranging system. As people know, an ultrasonic ranging system, which has a bell-shaped magnitude spectrum, acts like a band-pass filter. If the spectrum of the excitation signal does not match that of the ultrasonic ranging system, some of energy cannot be transmitted by the ultrasonic system. In other words, it does not make full use of the bandwidth of the ultrasonic ranging system. The good correlation characteristics can eliminate cross-talk among multichannel ultrasonic sensors firing simultaneously. To the authors’ knowledge, not many researchers considered how to make the spectrum of the excitation sequence match to that of the ultrasonic ranging system as well as improve the correlation characteristics. In this paper, the chaotic frequency modulation (CFM) excitation sequences are proposed for multiple-user ultrasonic ranging system. To obtain the excitation sequences which are spectrally matched to the ultrasonic ranging system as well as have the best correlation characteristic, the NSGA-II is applied to optimize the CFM excitation sequences.



2012 ◽  
Vol 75 (9) ◽  
pp. 1715-1720 ◽  
Author(s):  
LORRAINE McINTYRE ◽  
ELENI GALANIS ◽  
KIRSTEN MATTISON ◽  
OKSANA MYKYTCZUK ◽  
ENRICO BUENAVENTURA ◽  
...  

We describe the investigation of a norovirus outbreak associated with raw oyster consumption affecting 36 people in British Columbia, Canada, in 2010. Several genotypes were found in oysters, including an exact sequence match to clinical samples in regions B and C of the norovirus genome (genogroup I genotype 4). Traceback implicated a single remotely located harvest site probably contaminated by ill shellfish workers during harvesting activities. This outbreak resulted in three recalls, one public advisory, and closure of the harvest site.



2008 ◽  
Vol 20 (3) ◽  
pp. 291-300 ◽  
Author(s):  
David A. Pearce

AbstractSeven independent clone libraries were constructed to study the biodiversity of the bacterioplankton in the surface waters around Southern Thule, South Sandwich Islands, in order to identify the species present, to determine the sample effort required to estimate the total diversity, and to determine whether the surface waters around Southern Thule represented a highly specialized local anomaly or a subset of the marine meta-community. In total, 672 clones generated 629 useable sequences. These 629 clones matched 278 different sequences deposited in the 16S rDNA sequence databases. The majority of the clones were related to marine microorganisms, many of which had been previously detected in permanently cold Arctic and Antarctic marine environments. Each clone library generated an average of 35.8 new sequence matches. 346 clones covered two-thirds of the total estimated diversity, while 438 clones covered three-quarters of the total estimated diversity. Above this number, the coverage tended to stabilize and a relatively large number of additional clones were required to improve coverage significantly, increasing at the rate of about one new sequence match per 100 new clones. Comparing the different clone libraries, eight matches occurred in each of the seven libraries, whilst fifty-five occurred in only one, suggesting that there might be a relatively small number of common dominant ubiquitous species, with a much larger underlying diversity or ‘seed bank’ from which this dominant diversity is drawn. This study suggests that the dominant bacterioplankton in the surface waters around Southern Thule represent a subset of the marine meta-community, whilst sub-dominant diversity appears to be a highly specialized local anomaly.



2005 ◽  
Vol 79 (4) ◽  
pp. 2151-2159 ◽  
Author(s):  
Ji Yuan ◽  
Paul K. M. Cheung ◽  
Huifang M. Zhang ◽  
David Chau ◽  
Decheng Yang

ABSTRACT Coxsackievirus B3 (CVB3) is the most common causal agent of viral myocarditis, but existing drug therapies are of limited value. Application of small interfering RNA (siRNA) in knockdown of gene expression is an emerging technology in antiviral gene therapy. To investigate whether RNA interference (RNAi) can protect against CVB3 infection, we evaluated the effects of RNAi on viral replication in HeLa cells and murine cardiomyocytes by using five CVB3-specific siRNAs targeting distinct regions of the viral genome. The most effective one is siRNA-4, targeting the viral protease 2A, achieving a 92% inhibition of CVB3 replication. The specific RNAi effects could last at least 48 h, and cell viability assay revealed that 90% of siRNA-4-pretreated cells were still alive and lacked detectable viral protein expression 48 h postinfection. Moreover, administration of siRNAs after viral infection could also effectively inhibit viral replication, indicating its therapeutic potential. Further evaluation by combination found that no enhanced inhibitory effects were observed when siRNA-4 was cotransfected with each of the other four candidates. In mutational analysis of the mechanisms of siRNA action, we found that siRNA functions by targeting the positive strand of virus and requires a perfect sequence match in the central region of the target, but mismatches were more tolerated near the 3′ end than the 5′ end of the antisense strand. These findings reveal an effective target for CVB3 silencing and provide a new possibility for antiviral intervention.



1988 ◽  
Author(s):  
G. Xuan ◽  
Q. Wang


Nature ◽  
1982 ◽  
Vol 299 (5880) ◽  
pp. 226-231 ◽  
Author(s):  
Andrew D. McLachlan ◽  
Jonathan Karn


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