Isolation and Study of Biodegradation Capability of Hydrocarbonoclastic Bacteria from Industrial Waste Lubrication Oil Contaminated Sites

A research was carried out on Isolation and study of biodegradation capability of hydrocarbonoclastic bacteria from industrial waste lubricant oil contaminated sites. 12 different bacterial cultures are isolated from the soil samples collected from liquid effluent dump site of black galaxy granite, chimakurthy, prakasam Dist, and auto motive lubrication oil replacement garages, Auto Nagar, Vijayawada. Among the 12isolates 10 isolates were found to have capability of degrading used industrial lubrication oil VG 320 collected from NSL textiles LTD, Guntur. Among the isolates tested the isolate no BG4 obtained from liquid effluent dump site gave the maximum biodegradation potential of 18.78mm2followed by BG7 of 15.36, BG1 of 14.07and BG8 of 10.56 mm2 and the isolate no AL1 from automotive lubrication oil replacement garage soil sample found to be 9.03mm2 degradation potential. These isolates were identified based on physical and biochemical characters as Pseudomonas, Bacillus, Proteus, Flavobacterium and Enterococcussps respectively. For the future, our study will be focused on several data like the species of isolates, the optimal activity of isolates to degrade several Industrial lubricant oils.

Enhancing lead adsorption in waste lubricant oil with activated clay as bleaching earth

Waste lubricant oil is categorized as hazardous waste that contains several contaminants such as polycyclic aromatic hydrocarbon, chlorinated hydrocarbon, and heavy metals. Among these contaminants, lead has been known as one of the contributive contaminants in used lubricant oil in varied concentrations. To remove this contaminant, adsorption process was carried out by using clay as natural adsorbent (bleaching earth). In order to enhance the adsorption efficiency, the clay was activated by acidification with 1 M, 1.5M, 2 M H2SO4 or calcination in the temperature 300°C, 450°C, 600°C. The activated clay indicated the increase of montmorillonite percentage in clay up to 68% and clay’s surface area up to 67 m2/g from its origin characteristics. The adsorption process showed notable improvement of clay adsorption capacity in removing lead in shorter contact time. The lead removal curve showed that the optimum adsorption condition can be obtained by the use of activated clay with 1.5 M H2SO4 and calcined in temperature 450°C. The optimum adsorption contact time with acid and calcination activated clay was 30 minutes and 180 minutes respectively.