Phylogenetic Relationships within Uvularia (Colchicaceae) Based on Morphology

The five species of bellworts in the genus Uvularia are common woodland herbs endemic to eastern North America. Two previous studies conducted phylogenetic analyses of Uvularia based on matK and rbcL gene sequences. The objective of this study was to create a phylogeny for Uvularia based on 18 qualitative morphological characters and 14 quantitative characters. Two phylogenetic analyses were performed, one including all 32 characters and one excluding the 14 quantitative characters. Strict consensus trees from the two analyses had the same topology and were congruent with published molecular phylogenies, supporting two sections of the genus.

Migration of vascular plant species to a recent wood adjoining ancient woodland

Woodland communities can be restored by natural succession in sites adjoining ancient woodlands which can act as seed sources for trees, shrubs and woodland herbs. The influence of dominant tree species and the distance from an adjacent ancient oak-hornbeam woodland upon the floristic composition of species in a recent pine wood planted on dry rendzina soil were studied. It was found that, in spite of a 52-year long succession, the border between woods was sharp and the composition of species in the recent wood were significantly different than in the adjacent ancient woodland. Canonical correspondence analysis (CCA) showed that the distance to the ancient woodland had a significant influence on species distributions in the recent wood. The numbers of species from the <em>Querco-Fagetea</em> class, vegetatively reproducing species and myrmecochores decreased with this distance, whereas the numbers of anemochores increased. The migration rate of many woodland species, calculated on occurrence of the farthest individuals was very slow, varying from 0.0 m year^-1 to 0.38 m year^-1. The restoration of the field layer vegetation in the studied pine wood was much slower than in recent deciduous woods on rich and moist soils where the migration rate of some species exceeded 1.50 m year^-1. Recent woods adjacent to ancient woodlands can be more effectively colonised by woodland species only when they are dominated by broad-leaved trees with quickly decomposing litter, and the spatial continuity of these woods persists for a long period.

The shape of things to come: woodland herb niche contraction begins during recruitment in mesic forest microhabitat

Natural abundance is shaped by the abiotic requirements and biotic interactions that shape a species' niche, yet these influences are rarely decoupled. Moreover, most plant mortality occurs during early life stages, making seed recruitment critical in structuring plant populations. We find that natural abundance of two woodland herbs, Hexastylis arifolia and Hepatica nobilis , peaks at intermediate resource levels, a pattern probably formed by concurrent abiotic and biotic interactions. To determine how this abundance patterning reflects intrinsic physiological optima and extrinsic biotic interactions, we translocate adults and seeds to novel locations across experimentally extended abiotic gradients. These experiments indicate that the plant distributions probably reflect biotic interactions as much as physiological requirements, and that adult abundance provides a poor indication of the underlying niche requirements. The positive response exhibited by adult transplants in the wettest conditions is offset by increased fungal attack on buried seeds consistent with peak natural abundance where soil moisture is intermediate. This contraction of niche space is best described by Connell's model—species are limited by physiological tolerances where resources are low and biotic interactions where resources are high.

Temperate forest herbs are adapted to high air humidity — evidence from climate chamber and humidity manipulation experiments in the field

How growth and morphology of wild plants are controlled by the water vapor saturation deficit of the air (vapor pressure deficit, VPD) is not sufficiently understood. We tested the hypothesis that VPD acts on temperate woodland herbs independently from soil moisture by exposing two species ( Mercurialis perennis L. and Stachys sylvatica Torr.) to variable VPD levels in climate chambers and in open-top chambers on the forest floor. A decrease in air humidity from 85% to 40% in the climate chamber experiment, which simulates a microclimate change after canopy gap creation, resulted in a 40% decrease in biomass production of both species when grown in hydroponic culture. This result is supported by the more realistic open-top chamber experiment, which showed a productivity decrease by approximately 25% when plants were continuously exposed to an atmosphere with 15% lower air humidity than ambient. Elevated VPD levels reduced biomass production through either a reduced leaf expansion rate or a lower number of formed leaf buds. We conclude that many woodland herbs require sufficiently high air humidity for optimal growth; permanently decreased air humidity, as may occur in a future drier climate, after gap creation, or after clear-cutting of the forest, may threaten the vitality and survival of woodland herbs.

Effects of Forest Cover on Fruit Set in the Woodland Herb, Maianthemum canadense (Liliaceae)

Animal pollination has been recognized as an essential ecosystem function that is potentially under various environmental stresses. We investigated the landscape effects of forest cover at multiple spatial scales on the sexual reproductive success of a common woodland herb in North America, Maianthemum canadense. This species is a self-incompatible species and pollinated by insects requiring natural landscapes. Nine populations were selected in deciduous forests within agricultural fields of southern Ontario, Canada. We investigated whether fruiting success decreases as forest cover surrounding the plant populations increases at the landscape scale. Forest cover was quantified by the proportion of forest within six different radii from 250 to 1500 m. Analyses showed relationships with the proportion of forest at 750- and 1000-m radii and fruiting success in populations of M. canadense. These findings suggest potential local extirpation of M. canadense and indicate that forest loss can negatively impact on even some common woodland herbs.