Effect of sliding discharge on proliferation and death of brain microvessel endothelial cells in vitro

The dose-dependent effects of plasma exposure to a unipolar nanosecond sliding discharge over the surface of the culture medium in a closed plate on the cells of cerebral endothelium in vitro were studied. Using a 24-well plate, the surface plasma energy density of one pulse was 360 μJ/cm2 at a pulse frequency of 100 Hz. It has been shown that in the creeping discharge plasma there is an active excitation of air molecules, the formation of positive nitrogen and oxygen ions, and the formation of carbon monoxide. In the dose density range of 0-32 J/cm2, the dose-dependent effects were assessed in the 4-12 h post-radiation period. Cell death was analyzed with an assessment of the total number of cells, necrotic cells, cells in apoptosis (phosphatidylserine externalization, internucleosomal DNA fragmentation) and their proliferative activity (Ki67-immunopositive cells). A preliminary assessment of subtle dose-dependent effects indicates the peculiarities of the effect of small doses

Возникновение усилий на клине при взаимодействии скользящего разряда со сверхзвуковым потоком

It was investigated generation of forces on the wedge during the interaction of a sliding discharge in the supersonic flow. Sliding discharge was generated in a supersonic atmospheric-vacuum wind tunnel with a flow Mach number M = 2, Reynolds number of about 106, and static pressure in the flow p = 0.15 bar. It was found that the impact of the discharge is not limited by the appearance of a shock wave, and also significantly changes the properties of the flow around the wedge due to pulse heat generation. As the discharge energy increases, the force from its impact on the wedge also increases. Force produced by the sliding discharge in the supersonic flow is several times greater than in the quiescent air regardless of its pressure: 0.15 bar or 1 bar.