Ideal conditions for increased trapping success of pygmy rabbits (Brachylagus idahoensis) across the Great Basin

Sagebrush-steppe ecosystems are one of the most imperiled ecosystems in North America and many of the species that rely on these habitats are of great conservation concern. Pygmy rabbits (Brachylagus idahoensis) are one of these species. They rely on sagebrush year-round for food and cover, and are understudied across their range in the intermountain west due in part to their recalcitrance to standard capture techniques. Identifying an efficient and minimally biased trapping method therefore is a critical first step in learning more about this species. We assessed how trap orientation and weather characteristics influenced trap success for Tomahawk traps placed in and around pygmy rabbit burrows by carrying out trapping surveys at 16 occupied pygmy rabbit sites across the Great Basin from 2016 to 2018. We found that pygmy rabbits had a greater probability of being captured in traps with the open end facing away from burrow entrances. Pygmy rabbits also were more likely to be captured on clear days (0–5% cloud cover) and during periods of cooler temperatures during summer months (June–August). We found no evidence that sex or age ratios differed, or that individuals differed meaningfully, in their preference for certain trap orientations. To increase trap success for pygmy rabbits, we suggest maximizing trapping effort during summer months, at dawn, and maximizing the proportion of Tomahawk traps facing away from burrow entrances. We anticipate that our monitoring protocol will enable more effective research into the ecology and conservation of this cryptic and potentially imperiled species.

Evolution of CCL11: genetic characterization in lagomorphs and evidence of positive and purifying selection in mammals

The interactions between chemokines and their receptors are crucial for differentiation and activation of inflammatory cells. CC chemokine ligand 11 (CCL11) binds to CCR3 and to CCR5 that in leporids underwent gene conversion with CCR2. Here, we genetically characterized CCL11 in lagomorphs (leporids and pikas). All lagomorphs have a potentially functional CCL11, and the Pygmy rabbit has a mutation in the stop codon that leads to a longer protein. Other mammals also have mutations at the stop codon that result in proteins with different lengths. By employing maximum likelihood methods, we observed that, in mammals, CCL11 exhibits both signatures of purifying and positive selection. Signatures of purifying selection were detected in sites important for receptor binding and activation. Of the three sites detected as under positive selection, two were located close to the stop codon. Our results suggest that CCL11 is functional in all lagomorphs, and that the signatures of purifying and positive selection in mammalian CCL11 probably reflect the protein’s biological roles.

Mental Hygienic Aspects of Animal Assisted Education

The Institution for Special Education at the Faculty of Pedagogic of the University of Kaposvár has been engaged in animal assisted activities for about three years. Our most recent research program was conducted for over two month in the Spring of 2014 with the involvement of 66 children - all kindergarten and elementary school age -, 11 special educators, physicians, psychologists, special educators, teachers, ethologists. The primary focus of our research was the development (and examination) of memory however observations with ethological and mental hygiene angles were a natural segment of our work. A significant part of the observations pointed to factors that both the children and their educators have experienced: the acceptance of each-other, an increased level of tolerance, an increased attention level towards the partner (human and animal). The teachers gave account of their respective observations in a small conference at the end of the last school year. Researches were offered a glimpse into the unique world of the relationship between a part of “living nature” - the pygmy rabbit in our case - and humans. During the 12 sessions of the training our colleagues have made observations that could serve as basis for a new system of paradigms of animal assisted pedagogics in the future. Our experience can also be re-considered with aspects of remedial pedagogics: we are convinced that animal assistance can become an accentuated part of the care of children and students with impairments. This is also implied by the fact that preparatory works for the continuation of this research at a kindergarten and at a school are already in progress.

Evolutionary Insights into IL17A in Lagomorphs

In leporids, IL17A had been implicated in the host defense against extracellular pathogens, such asFrancisella tularensisthat infects hares and rabbits and causes the zoonotic disease tularemia. Here, we studied IL17A from five lagomorphs, European rabbit, pygmy rabbit, brush rabbit, European brown hare, and American pika. We observed that this protein is highly conserved between these species, with a similarity of 97–99% in leporids and ~88% between leporids and American pika. The exon/intron structure, N-glycosylation sites, and cysteine residues are conserved between lagomorphs. However, at codon 88, one of the interaction sites between IL17A and its receptor IL17RA, there is an Arg>Pro mutation that only occurs in European rabbit and European brown hare. This could induce critical alterations in the IL17A structure and conformation and consequently modify its function. The differences observed between leporids and humans or rodents might also represent important alterations in protein structure and function. In addition, as for other interleukins, IL17A sequences of human and European rabbit are more closely related than the sequences of human and mouse or European rabbit and mouse. This study gives further support to the hypothesis that European rabbit might be a more suitable animal model for studies on human IL17.

Predicting Occupancy for Pygmy Rabbits in Wyoming: An Independent Evaluation of Two Species Distribution Models

Species distribution models are an important component of natural-resource conservation planning efforts. Independent, external evaluation of their accuracy is important before they are used in management contexts. We evaluated the classification accuracy of two species distribution models designed to predict the distribution of pygmy rabbit Brachylagus idahoensis habitat in southwestern Wyoming, USA. The Nature Conservancy model was deductive and based on published information and expert opinion, whereas the Wyoming Natural Diversity Database model was statistically derived using historical observation data. We randomly selected 187 evaluation survey points throughout southwestern Wyoming in areas predicted to be habitat and areas predicted to be nonhabitat for each model. The Nature Conservancy model correctly classified 39 of 77 (50.6%) unoccupied evaluation plots and 65 of 88 (73.9%) occupied plots for an overall classification success of 63.3%. The Wyoming Natural Diversity Database model correctly classified 53 of 95 (55.8%) unoccupied plots and 59 of 88 (67.0%) occupied plots for an overall classification success of 61.2%. Based on 95% asymptotic confidence intervals, classification success of the two models did not differ. The models jointly classified 10.8% of the area as habitat and 47.4% of the area as nonhabitat, but were discordant in classifying the remaining 41.9% of the area. To evaluate how anthropogenic development affected model predictive success, we surveyed 120 additional plots among three density levels of gas-field road networks. Classification success declined sharply for both models as road-density level increased beyond 5 km of roads per km-squared area. Both models were more effective at predicting habitat than nonhabitat in relatively undeveloped areas, and neither was effective at accounting for the effects of gas-energy-development road networks. Resource managers who wish to know the amount of pygmy rabbit habitat present in an area or wanting to direct gas-drilling efforts away from pygmy rabbit habitat may want to consider both models in an ensemble manner, where more confidence is placed in mapped areas (i.e., pixels) for which both models agree than for areas where there is model disagreement.