The Effects of Particle Gradation on Salinized Soil in Arid and Cold Regions

Particle size grading impacts salt-frost heaving and dissolution collapse events of salinized soil on northwestern China’s arid and cold region highways. However, the influencing mechanisms remain unclear and the impact of varying particle size grading needs further investigation. Hence, this study focused on these effects and the number of freeze–thaw cycles on the characteristic changes in highway salinized soil in arid and cold regions. Three soil columns with different gradations were prepared to explore the gradation and the number of freeze–thaw cycle affects on salinized soil’s salt-frost heaving and dissolution collapse characteristics. The multi-functional physical simulation platform conducted multiple freeze–thaw cyclic tests in the laboratory. Test results confirmed significant and conclusive effects of gradation and the number of freeze–thaw cycles on salinized soil’s salt-frost heaving and dissolution collapse behaviors. Poorly graded salinized soil with high coarse particle content caused repeated freeze and thaw engineering hazards, significantly affecting salinized soil’s displacement and deformation behaviors during freezing. Contrarily, an increased range of fine particles more easily involved the characteristics of salinized soil during thawing. Therefore, the fourth freeze–thaw cycle was a crucial time node. After four freeze–thaw cycles, the displacement and deformation of original salinized soil and B-grade salinized soil samples (poorly graded with high fine particle content) tended to be stable. In contrast, the displacement and deformation of A-grade salinized soil samples (poorly graded with high coarse particle content) increased the growth rate. The present research results contribute to in-depth knowledge of the effects of gradation and freeze–thaw cycles on the characteristics of salinized soil in northwestern China, providing excellent referenced data support for the prevention and control of highway salinized soil failures and other engineering projects in arid and cold regions of northwest China.

An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos

Cryoprotection is of interest in many fields of research, necessitating a greater understanding of different cryoprotective agents. Antifreeze proteins have been identified that have the ability to confer cryoprotection in certain organisms. Antifreeze proteins are an evolutionary adaptation that contributes to the freeze resistance of certain fish, insects, bacteria, and plants. These proteins adsorb to an ice crystal's surface and restrict its growth within a certain temperature range. We investigated the ability of an antifreeze protein from the desert beetle Anatolica polita, ApAFP752, to confer cryoprotection in the frog Xenopus laevis. X. laevis eggs and embryos microinjected with ApAFP752 exhibited reduced damage and increased survival after a freeze/thaw cycle in a concentration-dependent manner. We also demonstrate that ApAFP752 localizes to the plasma membrane in eggs and embryonic blastomeres and is not toxic for early development. These studies show the potential of an insect antifreeze protein to confer cryoprotection in amphibian eggs and embryos.

Freeze-Thaw Behavior of Stabilized Clayey Soil with Red Mud and Cement

The clayey soils in areas with seasonal frost are exposed to at least one freeze-thaw cycle every year and worsen their engineering properties. To prevent the engineering properties of clayey soils, it is necessary to improve the freeze-thaw resistance of them. In this study, the clayey soil was stabilized by using red mud and cement additive materials. Prepared samples of clayey soil and stabilized clayey soil were subjected to the unconfined compressive test. To investigate the effects of red mud and cement additive materials on the freeze-thaw resistance of clayey soil, the natural and stabilized expansive soil samples were exposed to the freeze-thaw cycles under laboratory conditions. The obtained results showed that the red mud and cement additive materials increased the freeze-thaw resistance of clayey soil. Consequently, it was concluded that red mud and cement additive materials can be successfully used to improve the freeze-thaw resistance of clayey soils.

Healing of Chronic Wounds with Platelet-Derived Growth Factors from Single Donor Platelet-Rich Plasma following One Freeze-Thaw Cycle. A Cross-Sectional Study

Chronic non-healing wounds (CNHWs) may be associated with trauma or idiopathic in nature and are difficult to treat. Our objective was to assess the use of platelet-derived growth factor (PDGF) from single-donor platelets (al-PRP), using one freeze-thaw cycle, for treating CNHWs. We conducted a cross-sectional study. A total of 23 CNHWs being treated with al-PRP. The al-PRP treatment can be considered successful in well over half (n = 13, 56.5%) of the wounds. We found that all the wounds treated for up to 7 weeks showed partial or complete healing, while those treated for between 8 and 12 weeks did not show healing, healing again being successful in cases in which treatment was extended to more than 13 weeks (85.7%). Using chi-square tests, this relationship was found to be highly significant (p < 0.001, chi2 = 19.51; p value = 0.00006). Notably, Cramer’s V coefficient was very high (0.921), indicating that the effect size of PRP treatment duration on healing is very large (84.8%). We could suggest that the use of al-PRP in the healing of CNHWs is a promising approach. Further studies with larger sample sizes and long follow-ups are needed to obtain multivariate models to explain which factors favour the healing of ulcers treated with PRP

Study on Road Performance of Cement Fly Ash Stabilized Steel Slag—Concrete Recycled Macadam

In order to promote the application of steel slag in road engineering, improve its utilization rate and solve the environmental problems caused by its large accumulation, unconfined compressive strength (UCS) test, indirect tensile strength (ITS) test, freeze-thaw cycle test, dry shrinkage and temperature shrinkage test tests with different steel slag contents were carried out. And the strength formation mechanism of steel slag in base material was revealed by SEM. The results show that the strength of the mixture initially increased and then decreased with increasing steel slag content. The frost resistance increased with increasing steel slag content, which should be limited to no more than 75%. Increasing the steel slag content improved the drying shrinkage resistance but was not conducive to the temperature shrinkage resistance. Microscopic analysis shows that adding a suitable amount of steel slag generated a gel material that was distributed inside the pores. This increased the density of the hardened slurry structure, which improved the strength. The research can provide scientific basis for the application and promotion of steel slag in road base.

Polyphenol Loaded W1/O/W2 Emulsions Stabilized with Lesser Mealworm (Alphitobius diaperinus) Protein Concentrate Produced by Membrane Emulsification: Stability under Simulated Storage, Process, and Digestion Conditions

Water-in-oil-in-water (W1/O/W2) emulsions are complex delivery systems for polyphenols amongst other bio-actives. To stabilize the oil–water interphase, dairy proteins are commonly employed, which are ideally replaced by other, more sustainable sources, such as insect proteins. In this study, lesser mealworm (Alphitobius diaperinus) protein concentrate (LMPC) is assessed and compared to whey protein (WPI) and pea protein (PPI), to stabilize W1/O/W2 emulsions and encapsulate a commercial polyphenol. The results show that LMPC is able to stabilize W1/O/W2 emulsions comparably to whey protein and pea protein when using a low-energy membrane emulsification system. The final droplet size (d4,3) is 7.4 μm and encapsulation efficiency is between 72 and 74%, regardless of the protein used. Under acidic conditions, the LMPC shows a similar performance to whey protein and outperforms pea protein. Under alkaline conditions, the three proteins perform similarly, while the LMPC-stabilized emulsions are less able to withstand osmotic pressure differences. The LMPC stabilized emulsions are also more prone to droplet coalescence after a freeze–thaw cycle than the WPI-stabilized ones, but they are the most stable when exposed to the highest temperatures tested (90 °C). The results show LMPC’s ability to stabilize multiple emulsions and encapsulate a polyphenol, which opens the door for application in foods.

Experimental study of the freeze thaw characteristics of expansive soil slope models with different initial moisture contents

This paper presents an experimental investigation on the effect of freeze–thaw cycling on expansive soil slopes with different initial moisture contents. Clay soil from Weifang, China, was remolded and selected to build the expansive soil slope for the indoor slope model tests. A total of five freeze–thaw cycles were applied to the three expansive soil slopes with different moisture contents ranging from 20 to 40%. Variations of the crack developments, displacements, soil pressures and moisture contents of the expansive soil slope with different initial moisture contents during the freeze–thaw cycling were reported and discussed. The results indicate that higher moisture contents can slow the development of cracks and that the soil pressure increases with decreasing temperature. The soil pressure of slope decreases after freeze–thaw cycle, and the change amplitude of soil pressure after freeze–thaw is proportional to water content. The slopes with a moisture content of 20% and 30% shrinks during freezing and expands during thawing, which was named ES-FSTE Model, while the slope with a 40% moisture content shows the opposite behavior. During freeze–thaw cycles, moisture migrates to slope surface. As initial moisture contents increase, the soil heat transfer rate and bearing capacity decreases after five freeze–thaw cycling.