Morphological differences in the commissural connections of the forebrain in white outbred mice and BALB/C mice

BACKGROUND: The study of the mechanisms of interaction of paired structures of the mammalian brain is a fundamental problem of modern neuroscience, which is of great applied importance. Even mild underdevelopment of the corpus callosum in humans can lead to autism. It is known that the intensity of intraspecific interactions in BALB/c mice is lower than in white outbred ones, while some BALB/c substrains are characterized by underdevelopment of the corpus callosum. AIM: To compare the morphological parameters of the large brain commissures in white outbred mice and BALB/c mice grown in the Rappolovo nursery (Leningrad region). MATERIALS AND METHODS: The morphology of the corpus callosum was studied in 13 male white outbred mice and 7 male BALB/c mice at the age of 8 months. RESULTS: In mice of both subpopulations, the area of the anterior commissure of the left hemisphere was smaller than that of the right hemisphere (p 0.05). There were no differences between subpopulations in this parameter. The area of the left section of the corpus callosum trunkus in outbred mice was larger than the right one (p 0.001), while in BALB/c mice the areas of the left and right slices did not differ. Despite the absence of significant differences in the area of the anterior part (rostrum et genu) of the corpus callosum the density of the location of oligodendrocytes in this brain structure in the mice of the two subpopulations was different. The number of oligodendrocytes in 0.01 mm2 on the left section of the anterior part of the corpus callosum in BALB/c mice was greater than in white outbred mice (p 0.05). A similar trend was revealed when comparing slices of the right hemisphere (p = 0.065). CONCLUSIONS: The large area of the right parasagittal slice of the anterior commissure suggests that some of its constituent fibers do not cross the midline, but end within the same hemisphere, which may be the morphological basis for the functional dominance of the temporal cortex of the left hemisphere in mice of both subpopulations. The corpus callosum in BALB/c mice is developed symmetrically, and in white outbred ones asymmetrically. This feature may be the morphological basis for the functional dominance of the parietal cortex of the right hemisphere in outbred animals.

Thoracic esophageal injury due to a neck stab wound: a case report

Background Traumatic esophageal injury leads to severe complications such as mediastinitis, pyothorax, and tracheoesophageal fistula. Although prompt diagnosis and treatment are required, there are no established protocols to guide diagnosis or treatment. In particular, thoracic esophageal injury tends to be diagnosed later than cervical esophageal injury because it has few specific symptoms. We report a case of thoracic esophageal injury caused by a cervical stab wound; the patient was stabbed with a sharp blade. Case presentation A 74-year-old woman was attacked with a knife while sleeping at home. The patient was taken to the emergency room with an injury localized to the left section of her neck. She was suspected of a left jugular vein and recurrent laryngeal nerve injury from cervical hematoma and hoarseness. On the day following the injury, computed tomography revealed a thoracic esophageal injury. Emergency surgery was performed for an esophageal perforation and mediastinal abscesses. Although delayed diagnosis resulted in suture failure, the patient was able to resume oral intake of food a month later following enteral feeding with a gastrostomy. Esophageal injuries due to sharp trauma are rare, and most are cervical esophageal injuries. There are very few reports on thoracic esophageal injuries. Conclusions The possibility of thoracic esophageal injury should always be considered when dealing with neck stab wounds, particularly those caused by an attack.

Penilaian Kondisi Jalan Poros Sabbang Selatan Menggunakan Metode Surface Distress Index

Prasarana jalan jika terbebani volume lalu lintas yang tinggi dan berulang-ulang akan menyebabkan terjadinya penurunan kualitas jalan sehingga dapat mempengaruhi keamanan, kenyamanan dan kelancaran dalam berlalu lintas. Untuk menjaga agar tidak terjadi penurunan kondisi khususnya pada jalan poros Kecamatan Sabbang Selatan Kabupaten Luwu Utara tepatnya di jalan Padang Sarre, Buntu Terpedo sampai jalan Dandang sepanjang 4 km perlu adanya penanganan. Maka perlu dilakukan penelitian awal terhadap kondisi permukaan jalan dengan melakukan survei secara visual dengan cara menganalisa kerusakan berdasarkan jenis dantingkat kerusakannya. Tujuan penelitian yaitu menilai kondisi perkerasan danpenanganan sesuai kondisi permukaan jalan. Penelitian ini menggunakan system penilaian kondisi perkerasan menurut Bina Marga dengan perhitungan Surface Distress Index (SDI) untuk jalan beraspal. Dari hasil penelitian di dapatkan penilaian untuk jenis kerusakan permukaan jalan pada ruas kanan yaitu retak pinggir 1,183%, lubang 0,031%, amblas 0,054%, retak kulit buaya 3,271%, retak kotak-kotak 3,222%, tambalan 0,033% dan pengelupasan butir 0,013%. Sedangkan untuk ruas kiri yaitu retak pinggir 0,035%, lubang 0,051%, amblas 0,000%, retak kulit buaya 0,130%, retak kotak-kotak 2,351%, tambalan 0,000% dan pengelupasan butir 0,150%. Kondisi perkerasan jalan yang menjadi objek penelitian sepanjang 4 km yaitu 85% baik, 0% sedang, 15% rusak ringan, 0% rusakberat.Road infrastructure if it is burdened by high and repetitive traffic volumes will cause a decrease in road quality so that it can affect safety, comfort and smoothness in traffic. To prevent deterioration in conditions, especially on the axis road of South Sabbang District, North Luwu Regency, precisely on Padang Sarre road, Buntu Terpedo to Dandang road along 4 km, it needs handling. So it is necessary to conduct an initial research on road surface conditions by conducting a visual survey by analyzing the damage based on the type and level of damage. The research objective was to assess pavement conditions and handling according to road surface conditions. This study uses a pavement condition assessment system according to Bina Marga with the calculation of the Surface Distress Index (SDI) for asphalt roads. From the research results obtained an assessment for the type of road surface damage on the right side, namely edge cracks 1.183%, holes 0.031%, collapse 0.054%, crocodile skin cracks 3.271%, checkered cracks 3.222%, 0.033% patches and 0.013% peeling grains. Whereas for the left section, the edges cracked 0.035%, holes 0.051%, collapsed 0.000%, crocodile skin cracks 0.130%, checkered cracks 2.351%, fillings 0.000% and peeling 0.150%. The condition of the pavement which is the object of the research along 4 km is 85% good, 0% moderate, 15% lightly damaged, 0% heavily damaged.

Abstract 571: Short Term Taste Preference for Cholesterol Rich Diet in ABCA1 Null Mice

Background: Plasma HDL particle is one of the essential player for maintaining cholesterol homeostasis in mammal. Even though cholesterol can be ubiquitously biosynthesized upon requirement, cholesterol in diet is efficiently used to maintain cholesterol homeostasis which implicates existence of sensing system for cholesterol in diet. The study of taste preference on cholesterol was not well explored. In this project we examined taste preference in mice using a novel method called dual diet test (DDT) system. Methods: Chow pellets of 1.2% cholesterol or 0.02% Acesulfame potassium (AmK, calorie-free sweet molecule) were prepared based on standard mouse chow, CE-2(0.2% cholesterol, Crea Japan). The mouse chow tray was divided into three sections. Water nozzle was set in the center section. Each chow was weighed then set ether right or left section. One session was composed by 1-3 days and remaining chow was weighed to calculate consumption of each chow diet. The diet position was switched randomly at each session. The operation was performed between ZT9-ZT10 when mice were less active in a day. To avoid social isolation stress, 2-5 littermates were placed per cage. Results: C56B/6 wild type mice and LCAT null mice significantly preferred 1.2% cholesterol containing chow compared to control chow during 6 sessions by 18-28 weeks old littermates (n=16, n=8, respectively). These mice also significantly preferred AmK-chow compared to the control chow. ABCA1 null and ABCA1 heterozygote mice exhibited significance on the preference of 1.2% cholesterol in the first 3 sessions but not for the later sessions. Repeating the experiment after interval period improved their cholesterol preference in ABCA1 null mice. No gender difference was observed. Preferences to AmK-chow were significant in all trials. Conclusions: The preference in higher cholesterol or sweet in diet were detected at this novel DDT system, significantly. In ABCA1 null or heterozygote mice, long-term preferences to high cholesterol diet were abolished perhaps due to their lack of focus on cholesterol-rich chow of which less reward to these mice. In this experiment, mice synchronized in consuming higher cholesterol containing diet indicating mice are sensing cholesterol as a preferred tastant.

Observations of Polarimetric Signatures in Supercells by an X-Band Mobile Doppler Radar

Polarimetric weather radars significantly enhance the capability to infer the properties of scatterers within a resolution volume. Previous studies have identified several consistently seen polarimetric signatures in supercells observed in the central United States. Nearly all of these studies used data collected by fixed-site S- and C-band radars. Because there are few polarimetric mobile radars, relatively little has been documented in high-resolution polarimetric data from mobile radars. Compared to S and C bands, there has been very limited examination of polarimetric signatures at X band. The primary focus of this paper is on one signature that has not been documented previously and one that has had little documentation at X band. The first signature, seen in at least seven supercell datasets collected by a mobile, X-band, polarimetric radar, consists of a narrow band of locally reduced reflectivity factor ZH and differential reflectivity, typically near the location where the hook echo “attaches” to the main body of the storm echo. No consistent pattern is seen in radial velocity VR or copolar cross correlation ρHV. The small size of this feature suggests a significant heterogeneity in precipitation microphysics, the cause and impact of which are unknown. The greater resolution and the scattering differences at X band compared to other frequencies may make this feature more apparent. The second signature consists of anomalously low ρHV in areas of high ZH along the left section (relative to storm motion) of the bounded weak-echo region. Examples of other polarimetric signatures at X band are provided.

Effects of unilateral or bilateral superior ovarian nerve section in prepubertal rats on the ovulatory response to gonadotrophin administration

The modulating effects of ovarian innervation reaching the ovary through the suspensory ovarian nerve on the reactivity of the ovaries to gonadotrophins were analysed. Juvenile rats (32 days old), with or without unilateral or bilateral section of the superior ovarian nerve, were injected with 8 iu of pregnant mare serum gonadotrophin (PMSG), 10 iu of human chorionic gonadotrophin (hCG) or with 8 iu of PMSG followed 56 h later with 10 iu of hCG. Treatments were given immediately after surgery or 4 days later, and the rats were killed on the day of first vaginal oestrus. In rats with unilateral section, treatment with PMSG did not induce full ovulatory response by the denervated ovary whether the treatment was applied immediately or 4 days after surgery (0/11 rats treated immediately ovulated vs 5/5 (sham) and 11/12 (control, P < 0.05 Fisher's exact probability test), and 4/19 did when treatment was done 4 days after surgery vs 8/10 (sham) and 11/12 (control, P < 0.05). The rats with bilateral section receiving the same hormonal treatment, PMSG administration, ovulated. The number of ova shed by the left ovary was similar to those of the control, while the right ovary released fewer ova. Stimulation with hCG immediately after unilateral section did not induce ovulation in normal or denervated ovary. When the treatment was applied 4 days after surgery, ovulation was observed only in the innervated ovary. In the rats with bilateral section, hCG injection induced ovulation in both ovaries. In those rats with unilateral section of the superior ovarian nerve, the treatment with PMSG + hCG given immediately after surgery resulted in a compensatory ovulation by the innervated ovary (the number of ova shed/ovulating animal was significantly higher than those released by control or sham-operated animals: left section, number of ova shed by the right ovary 7.6 +/- 0.3 vs 5.5 +/- 0.8 and 4.9 +/- 1 respectively, P < 0.05; right section, number of ova shed by the left ovary 10.2 +/- 0.6 vs 4.4 +/- 1.1 and 7.0 +/- 0.9, P < 0.05), while the denervated one showed a lower ovulation rate as well as a smaller number of ova shed than those by the control animals. When the hormonal replacement was given 4 days after surgery, such compensatory ovulation was observed in the left ovary of those rats with a section of the right nerve (14.3 +/- 2.6 vs 4.4 +/- 1.1 and 6.5 +/- 1.1, P < 0.05). When the PMSG + hCG treatment was applied to animals with bilateral section of the superior ovarian nerve, the ovulation rate by the right ovary was significantly lower than in control and sham-operated treated animals (2/10 vs 11/11 and 6/7, P < 0.05). Because the ovaries receive innervation through the superior ovarian nerve, the ovarian plexus and the vagus nerve, the results obtained in unilateral denervated animals suggest that the innervation of the ovary via the superior ovarian nerve regulates in a stimulatory way the effects of gonadotrophin resulting in ovulation. The ovulation induced by hormonal treatment of rats with bilateral section of the superior ovarian nerve suggests that the effects of bilateral section on ovulation are not the addition of the effects of left and right denervation, implying the existence of a modulatory effect in gonadotrophin action on ovulation via other neural pathways.