Aberrant integration of Hepatitis B virus DNA promotes major restructuring of human hepatocellular carcinoma genome architecture

Most cancers are characterized by the somatic acquisition of genomic rearrangements during tumour evolution that eventually drive the oncogenesis. Here, using multiplatform sequencing technologies, we identify and characterize a remarkable mutational mechanism in human hepatocellular carcinoma caused by Hepatitis B virus, by which DNA molecules from the virus are inserted into the tumour genome causing dramatic changes in its configuration, including non-homologous chromosomal fusions, dicentric chromosomes and megabase-size telomeric deletions. This aberrant mutational mechanism, present in at least 8% of all HCC tumours, can provide the driver rearrangements that a cancer clone requires to survive and grow, including loss of relevant tumour suppressor genes. Most of these events are clonal and occur early during liver cancer evolution. Real-time timing estimation reveals some HBV-mediated rearrangements occur as early as two decades before cancer diagnosis. Overall, these data underscore the importance of characterising liver cancer genomes for patterns of HBV integration.

Enhanced Breathing Pattern Detection during Running Using Wearable Sensors

Breathing pattern (BP) is related to key psychophysiological and performance variables during exercise. Modern wearable sensors and data analysis techniques facilitate BP analysis during running but are lacking crucial validation steps in their deployment. Thus, we sought to evaluate a wearable garment with respiratory inductance plethysmography (RIP) sensors in combination with a custom-built algorithm versus a reference spirometry system to determine its concurrent validity in detecting flow reversals (FR) and BP. Twelve runners completed an incremental running protocol to exhaustion with synchronized spirometry and RIP sensors. An algorithm was developed to filter, segment, and enrich the RIP data for FR and BP estimation. The algorithm successfully identified over 99% of FR with an average time lag of 0.018 s (−0.067,0.104) after the reference system. Breathing rate (BR) estimation had low mean absolute percent error (MAPE = 2.74 [0.00,5.99]), but other BP components had variable accuracy. The proposed system is valid and practically useful for applications of BP assessment in the field, especially when measuring abrupt changes in BR. More studies are needed to improve BP timing estimation and utilize abdominal RIP during running.