extracellular glycoproteins
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2021 ◽  
Author(s):  
Fangxu Sun ◽  
Suttipong Suttapitugsakul ◽  
Ronghu Wu


Author(s):  
Ming-Hua Zeng ◽  
Yan Wang ◽  
Hua-Lin Huang ◽  
Ru-Ping Quan ◽  
Jun-Ting Yang ◽  
...  

Abstract Zona pellucida (ZP), which is composed of at most four extracellular glycoproteins (ZP1, ZP2, ZP3, and ZP4) in mammals, shelters the oocytes and is vital in female fertility. Several studies have identified the indispensable roles of ZP1–3 in maintaining normal female fertility. However, the understanding of ZP4 is still very poor because only one study on ZP4-associated infertility performed in rabbits has been reported up to date. Here we investigated the function of mammalian Zp4 by creating a knockout (KO) rat strain (Zp4−/− rat) using CRISPR-Cas9 mediated DNA-editing method. The influence of Zp4 KO on ZP morphology and some pivotal processes of reproduction, including oogenesis, ovulation, fertilization and pup production, was studied using periodic acid–Schiff’s staining, superovulation, in vitro fertilization, and natural mating. The ZP morphology in Zp4−/− rats was normal and none of these pivotal processes was affected. This study renewed the knowledge of mammalian Zp4 by suggesting that Zp4 was completely dispensable for female fertility.



2003 ◽  
Vol 38 (4) ◽  
pp. 351-360 ◽  
Author(s):  
Anthony Chiovitti ◽  
Antony Bacic ◽  
Jo Burke ◽  
Richard Wetherbee


2002 ◽  
Vol 57 (7-8) ◽  
pp. 634-639 ◽  
Author(s):  
Emília Breierová ◽  
Ingrid Vajcziková ◽  
Vlasta Sasinková ◽  
Eva Stratilová ◽  
Miroslav Fišera ◽  
...  

Toxicity and accumulation of Cd2+ in yeasts were studied in eight different yeast species. The adaptation to toxic concentration of this metal was dependent on the production of extracellular yeast glycoproteins. The highest concentration of Cd2+ ions in the growth medium was tolerated by a Hansenula anomala, strain while the lowest tolerance was found by the strain of species Saccharomyces cerevisiae. Extracellular glycoproteins of Hansenula anomala absorbed nearly 90% of the total content of Cd2+ ions bound by yeast cells, while extracellular glycoproteins of Saccharomyces cerevisiae bound only 6% of the total amount of cadmium. This difference is caused by the variable composition of the saccharide moiety in the extracellular glycoproteins. The composition of extracellular glycoproteins changed during the adaptation of the yeast cells to the presence of Cd2+ ions.



Development ◽  
2001 ◽  
Vol 128 (24) ◽  
pp. 5031-5037
Author(s):  
Kalpana Patel ◽  
Julia A. B. Nash ◽  
Akira Itoh ◽  
Zhe Liu ◽  
Vasi Sundaresan ◽  
...  

Members of the Slit family are large extracellular glycoproteins that may function as chemorepellents in axon guidance and neuronal cell migration. Their actions are mediated through members of the Robo family that act as their receptors. In vertebrates, Slit causes chemorepulsion of embryonic olfactory tract, spinal motor, hippocampal and retinal ganglion cell axons. Since Slits are expressed in the septum and floor plate during the period when these tissues cause chemorepulsion of olfactory tract and spinal motor axons respectively, it has been proposed that Slits function as guidance cues. We have tested this hypothesis in collagen gel co-cultures using soluble Robo/Fc chimeras, as competitive inhibitors, to disrupt Slit interactions. We find that the addition of soluble Robo/Fc has no effect on chemorepulsion of olfactory tract and spinal motor axons when co-cultured with septum or floor plate respectively. Thus, we conclude that although Slits are expressed in the septum and floor plate, their proteins do not contribute to the major chemorepulsive activities emanating from these tissues which cause repulsion of olfactory tract and spinal motor axons.



1992 ◽  
Vol 118 (3) ◽  
pp. 275-283 ◽  
Author(s):  
F.R. Thormodsson ◽  
T.S. Parker ◽  
B. Grafstein


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