We present the results of the hydrochemical analysis in Lake Itkul, which is the natural monument in the Chelyabinsk Region and an important recreational facility for the population of the Chelyabinsk and Sverdlovsk Regions. The water of Lake Itkul is fresh in terms of chemical composition, has low-alkaline medium reaction, and is very soft and saturated with oxygen. The water content in biogenic substances such as ions of nitrite, nitrate, and phosphate is low. Description and analysis of ecological groups of phytoplankton species of Lake Itkul are provided. The phytoplankton of Lake Itkul comprises eurybiontic and neutral species having wide geographical distributions. Analysis of ion contents of the zinc, copper, manganese, and nickel in the water and organs and tissues of the common roach indicated high residual concentrations of nickel in fish skin and bones, above allowable values. The content of iron, manganese, and other secondary elements in water was significantly lower than their maximum allowable concentrations, reflecting the absence of industrial water discharge within the lake water intake.