Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines

Introduction and Aim: In 21stcentury, nanomedicine has turned out to be an emergent modulus operation for the diagnosis and treatment of cancer. The current study includes the optimization and green synthesis of zinc oxide nanoparticles from the leaves of Garcinia cambogia and interpretation of its antioxidant and anticancer activity. Materials and Methods: Synthesized ZnO NPs were investigated by UV–vis, FTIR, XRD, SEM, EDX, HR-TEM, SAED, particle size distribution and DLS analysis. Furthermore, different antioxidant assays were conducted for evaluating the antioxidant activity of (ZnO NPs) and its anticancer activity using (A498) cell lines along with non-cancerous (HEK-293) cell line using MTT assay and XTT assay. Results: The UV–visible spectrum showed an absorption peak at 277 nm that reflects surface plasmon resonance (SPR) ZnONPs. The presence of various functional groups was confirmed by FTIR analysis. XRD patterns indicated that the ZnO NPs had a single-phase nature with the wurtzite structure. SEM, TEM pictures confirmed the size and shape of synthesized Nanoparticles ranges of 11–32 nm and mostly hexagonal forms. The Zeta potential value of 57.9 mV revealed the surface charge of green synthesized ZnONPs. The cytotoxicity of the ZnO NPs with various concentrations (6.25 to 200 µg/mL) was measured in (A498) cell lines along with non-cancerous (HEK-293) cell line. After the exposure, to MTT and XTT assay, cellular morphology studies showed that the cancer cell viability decreased with increasing ZnO NP concentration. Conclusion: The synthesized ZnONPs via a green synthetic route could be developed as anti-cancer agent against cancer cells.

Circular RNA ABCB10 promotes tumor progression and correlates with pejorative prognosis in clear cell renal cell carcinoma

Objective: Our study aimed to evaluate the effect of circular RNA ABCB10 (circ-ABCB10) on proliferation and apoptosis of clear cell renal cell carcinoma (ccRCC) cells, and its prognostic value in patients with ccRCC. Methods: Circ-ABCB10 expression in five ccRCC cell lines and normal kidney epithelial cell line was measured by quantitative polymerase chain reaction (qPCR). Empty overexpression, circ-ABCB10 overexpression, empty shRNA, and circ-ABCB10 shRNA plasmids were transfected into A498 cells as negative control for circ-ABCB10 over expression {NC (+)}, Circ-ABCB10(+), negative control (−){NC(−)}, and Circ-ABCB10(−) groups, then cell proliferation and apoptosis were evaluated by Cell Counting Kit-8 and annexin V/propidium iodide. Meanwhile, apoptotic markers were measured by western blot. Subsequently, circ-ABCB10 expression in tumor tissues and paired adjacent tissues from 120 ccRCC patients was measured by qPCR. Results: Circ-ABCB10 expression was elevated in all the ccRCC cell lines compared with the normal kidney cells line. A498 cell proliferation was enhanced in the Circ-ABCB10(+) group compared with the NC(+) group, while it was inhibited in the Circ-ABCB10(−) group compared with the NC (−) group; and A498 cell apoptosis was repressed in the Circ-ABCB10(+) group than the NC(+) group, but was promoted in the Circ-ABCB10(−) group compared with the NC(−) group. In addition, circ-ABCB10 was up-regulated in tumor tissues compared with paired adjacent tissues, and its high expression correlated with the advanced pathological grade and the tumor node metastasis stage as well as independently predicting worse overall survival in ccRCC patients. Conclusion: Circ-ABCB10 promotes tumor progression and correlates with pejorative prognosis in ccRCC.