porcine erythrocyte
Recently Published Documents


TOTAL DOCUMENTS

28
(FIVE YEARS 0)

H-INDEX

10
(FIVE YEARS 0)

2018 ◽  
Author(s):  
Jingjing Zhang ◽  
Wei Yin ◽  
Chun Wang ◽  
Ruipu Jia ◽  
Kuohai Fan ◽  
...  

Background. Porcine erythrocytes express complement receptor 1-like (CR1-like), which is involved in immune adherence. Methods. In this study, porcine erythrocyte samples were collected from fifty-five individual Landrace swine to characterize differences in porcine CR1-like. Flow cytometry analysis was performed to examine the porcine differences in CR1-like expression density and immunoprecipitation, SDS-PAGE and Western blot were performed to detect variations in porcine CR1-like molecular weights. Results. Different mean fluorescence intensities (MFI) of porcine erythrocytes were identified in three groups as 33.016±2.889 (40.0%), 59.974±9.299 (45.5%) and 131.241±8.375 (14.5%). Under reduced condition, three porcine CR1-like molecular weight variants were identified as 85.280±0.935 kDa (9.09%), 123.939±2.752 kDa (14.55%) and 136.696±2.028 kDa (76.36%). Discussion. CR1-like was dispersed on the surface of porcine erythrocytes and promoted immune adherence. There have been no reports on whether differences in the expression levels and/or molecular weights of CR1-like in erythrocytes represent diversity in different individuals, and if so, whether this diversity influences the immune adherence of erythrocytes and/or whether the diversity is associated with CR1-like polymorphisms. At present, five candidate genes that are related to the differences above were found. Research examining erythrocyte immune adherence and CR1-like genes is under way. These results will provide theoretical data for future studies of the immunological mechanism of CR1-like in porcine erythrocytes.



2018 ◽  
Author(s):  
Jingjing Zhang ◽  
Wei Yin ◽  
Chun Wang ◽  
Ruipu Jia ◽  
Kuohai Fan ◽  
...  

Background. Porcine erythrocytes express complement receptor 1-like (CR1-like), which is involved in immune adherence. Methods. In this study, porcine erythrocyte samples were collected from fifty-five individual Landrace swine to characterize differences in porcine CR1-like. Flow cytometry analysis was performed to examine the porcine differences in CR1-like expression density and immunoprecipitation, SDS-PAGE and Western blot were performed to detect variations in porcine CR1-like molecular weights. Results. Different mean fluorescence intensities (MFI) of porcine erythrocytes were identified in three groups as 33.016±2.889 (40.0%), 59.974±9.299 (45.5%) and 131.241±8.375 (14.5%). Under reduced condition, three porcine CR1-like molecular weight variants were identified as 85.280±0.935 kDa (9.09%), 123.939±2.752 kDa (14.55%) and 136.696±2.028 kDa (76.36%). Discussion. CR1-like was dispersed on the surface of porcine erythrocytes and promoted immune adherence. There have been no reports on whether differences in the expression levels and/or molecular weights of CR1-like in erythrocytes represent diversity in different individuals, and if so, whether this diversity influences the immune adherence of erythrocytes and/or whether the diversity is associated with CR1-like polymorphisms. At present, five candidate genes that are related to the differences above were found. Research examining erythrocyte immune adherence and CR1-like genes is under way. These results will provide theoretical data for future studies of the immunological mechanism of CR1-like in porcine erythrocytes.



2012 ◽  
Vol 19 (5-6) ◽  
pp. 79-84 ◽  
Author(s):  
Yao-Gui Sun ◽  
Wei Yin ◽  
Xin-Feng Fan ◽  
Kuo-Hai Fan ◽  
Jun-Bing Jiang ◽  
...  


2002 ◽  
Vol 28 (8) ◽  
pp. 1081-1088 ◽  
Author(s):  
Subha Maruvada ◽  
K.Kirk Shung ◽  
Shyh-Hau Wang


1999 ◽  
Vol 67 (9) ◽  
pp. S556 ◽  
Author(s):  
Jay Doucet ◽  
Zu-hua Gao ◽  
Leslie MacLaren ◽  
Jamie Johnstone ◽  
Vivian McAlister
Keyword(s):  


1987 ◽  
Vol 19 (10) ◽  
pp. 923-929 ◽  
Author(s):  
Fumiyo Sako ◽  
Shinsei Gasa ◽  
Akira Makita


Lipids ◽  
1985 ◽  
Vol 20 (9) ◽  
pp. 588-593 ◽  
Author(s):  
Yasuo Suzuki ◽  
Naoto Suzuki ◽  
Hiroyasu Michi ◽  
Makoto Matsumoto


1985 ◽  
Vol 33 (8) ◽  
pp. 3510-3513
Author(s):  
TOSHIKATSU NAKABAYASHI ◽  
HIROH IKEZAWA ◽  
HARUKI OGAWA ◽  
MINORU SHINDO ◽  
ISAO MATSUNAGA


Sign in / Sign up

Export Citation Format

Share Document