ALCON

ALCON

ALLERGAN

ALLERGAN

Method to quantify the decentration of the multifocal intraocular lenses in relation to the visual axis

Since position and centration have shown to affect the effectiveness of multifocal intraocular lenses (MFIOLs), some authors have proposed using the "coaxially sighted IOL light reflex" as a landmark to allow consistent centration of MFIOLs. Postoperatively, assessing the location of the intraocular lens with respect to the visual axis is also important. Centration of the MFIOLs can be identified postoperatively by slit lamp biomicroscopy, but it is still a qualitative method. The Nidek OPD Scan III is used. The instrument obtains retro illumination images, which allows the observation of the diffractive rings of the multifocal intraocular lenses implanted following phacoemulsification. The retro illumination image describes the location of the visual axis (white cross with blue borders +), the center of the photopic (pink cross) and mesopic (turquoise cross) pupil, quantifies the distance between the center of the pupil and the visual axis, and places the visual axis in degrees. It is attempted to match the rings of the lens with that of the acetate grid. Once the best possible equidistance between the lens and grid rings has been achieved. This method can be useful to be able to have reference of a real value of decentration of the lenses and thus have a surgical plan and try to center the lenses. We are also creating a technique to properly center the lenses, in addition to performing an analysis of the results in terms of postoperative visual quality in relation to the lens's decentration and thus see how this really affects.

JOHNSON&JOHNSON

JOHNSON&JOHNSON

BILATERAL ABDUCENS NERVE PALSY SECUNDARY TO INTRACRANIAL SINUSES THROMBOSIS IN A CHILD WITH NEPHROTIC SYNDROM – CASE REPORT

We report a case of a child with nephrotic syndrome (NS), who presented an acute convergent strabismus, double vision and papilledema one week after a history of badly treated sinusitis. The image study revealed an extensive thrombosis of the intracranial sinuses that was treated with intravenous heparin and oral corticosteroids. Four months later, the case evolved with spontaneous resolution of the ocular deviation and no diplopia.

Metformin activity in an in vitro model of posterior capsule opacification

Purpose: To determine the activity of metformin in an in vitro model of posterior capsule opacification (PCO).Design: Experimental laboratory research. Methods: The HLE-B3 lens epithelial cell line was treated with PCO induction media (PCOM) supplemented with transforming growth factor-beta (TGF-β) and fibroblast growth factor (FGF). Different metformin concentrations (0-100 mM) were used. The following cellular parameters were assessed: (1) survival, using a viability assay; (2) morphology, via microscopy and image analysis; (3) migration, using the wound assay; (4) and expression of epithelial (Pax6, E-cadherin) and mesenchymal (α-smooth muscle actin or α-SMA, fibronectin) markers via Western blot. Expression of the uptake receptor SLC22A1 was evaluated in HLE-B3 and in human donor eyes with Western blot and immunohistochemistry, respectively. Statistical analysis of variance (ANOVA) with Tukey post-hoc test was done for analysis of cytotoxicity, morphology and migration data. Results: Metformin was lethal to half (LC50) of the cells at 30 mM, and a decrease in viability (P<0.05) was noted at 5 mM. LECs in PCOM treated with 1 mM metformin showed increased Pax6 and E-cadherin and decreased α-SMA and fibronectin expression. LECs in PCOM treated with metformin also maintained epithelial morphology. Migration was inhibited with 0.5 mM metformin (P<0.05). Both HLE-B3 and the lens epithelium in donor eyes were found to express SLC22A1.Conclusion: Metformin decreased survival and migration in LECs, maintaining epithelial phenotype and reducing mesenchymal marker expression. Metformin therefore has potential as an adjunct in PCO prevention.Financial Disclosures: This work was partially funded by Mitacs Canada.

MESSAGE FROM THE CHAIRMAN, PAOF BOARD

MESSAGE FROM THE CHAIRMAN, PAOF BOARD

Table of Contents

Table of Contents